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RNA-Guided CRISPR-Cas9 System for Removal of Microbial Pathogens

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Microbial Genomics in Sustainable Agroecosystems

Abstract

CRISPR-Cas9 technology has been cherished and well appreciated by the scientific community. The popularity of CRISPR-Cas9 technology is because it provides simple and efficient directions for genome engineering with feasible applications in a broad range of organisms. It stands to reason that the development of CRISPR-Cas9 is probably among the greatest revolution in the field of molecular biology, ever since the discovery of PCR. Genome engineering of microbes and other organisms may open up newer avenues to achieve a dynamic ecosystem. In this chapter, research on the use of CRISPR-Cas9 technology as an anti-phytopathogenic arsenal has been highlighted. Furthermore, the engineered organism developed using CRISPR-Cas9 technology has also been explained. Besides the applicative side, the background and molecular mechanisms of the CRISPR-Cas9 system have been mentioned and explained thoroughly.

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Acknowledgments

This work was supported by Puri Foundation for Education in India.

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Author notes

  1. Vijai Singh

    Present address: Department of Biosciences, School of Sciences, Indrashil University, Kadi, Gujarat, India

Authors and Affiliations

  1. School of Biological Sciences and Biotechnology, Institute of Advanced Research, Gandhinagar, India

    Gargi Bhattacharjee, Khushal Khambhati & Vijai Singh

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  1. Gargi Bhattacharjee
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  2. Khushal Khambhati
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  3. Vijai Singh
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Correspondence to Vijai Singh .

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Editors and Affiliations

  1. Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh, India

    Vijay Tripathi

  2. Department of Forestry, North Eastern Regional Institute of Science and Technology (Deemed To Be University-MHRD), Itanagar, Arunachal Pradesh, India

    Pradeep Kumar

  3. Department of Computational Biology and Bioinformatics, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh, India

    Pooja Tripathi

  4. Department of Botany, Kamla Nehru P.G. College, Raebareli, Uttar Pradesh, India

    Amit Kishore

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Bhattacharjee, G., Khambhati, K., Singh, V. (2019). RNA-Guided CRISPR-Cas9 System for Removal of Microbial Pathogens. In: Tripathi, V., Kumar, P., Tripathi, P., Kishore, A. (eds) Microbial Genomics in Sustainable Agroecosystems. Springer, Singapore. https://doi.org/10.1007/978-981-13-8739-5_12

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