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Combined Biological Effects of N-Bromotaurine Analogs and Ibuprofen. Part II: Influence on a Local Defense System

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Taurine 11

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1155))

Abstract

The stable N-bromotaurine analogs (N-dibromo-dimethyl taurine, N-monobromo-dimethyl taurine), and bromamine T (BAT) show anti-inflammatory and microbicidal properties. These bromamines are good candidates for a treatment of skin infectious/inflammatory diseases as local antiseptics. Ibuprofen, a non-steroidal anti-inflammatory drug (NSAID), is commonly used in various infectious/inflammatory diseases due to its analgesic and antipyretic therapeutic effects. However, systemic administration of ibuprofen may also result in adverse side effects. It has been reported that ibuprofen enhances serum levels of TNF-α and worsens secondary skin infections caused by invasive streptococci (S. pyogenes). Recently we have demonstrated that bromamines inhibit the stimulatory effect of ibuprofen on the production of inflammatory cytokines (TNF-α, IL-6). The aim of this study was to examine the combined antibacterial actions of ibuprofen and bromamines against S. pyogenes and their joint effect on the generation of reactive oxygen species (ROS) by activated neutrophils and macrophages. We have shown that the microbicidal activity of bromamines against S. pyogenes was not altered by ibuprofen. On the other hand, co-administration of ibuprofen and bromamines markedly decreased the generation of ROS by activated neutrophils and macrophages. Finally, we discuss how the antioxidant combined effect of bromamines and ibuprofen may affect a local defense system.

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Abbreviations

Tau:

taurine

HOBr:

hypobromous acid

Tau-NHBr:

N-bromotaurine, taurine bromamine

DM-NBr2T (Br-422):

N-dibromo-dimethyltaurine

BAT:

bromamine T, N-bromo-N-sodio-p-toluenesulfonamide

LCL:

luminol-dependent chemiluminescence

OZ:

opsonized zymosan

ROS:

reactive oxygen species

NO:

nitric oxide

PMN:

polymorphonuclear cells, murine peritoneal neutrophils

Mϕ:

murine peritoneal macrophages

S. pyogenes :

Streptococcus pyogenes

MBC:

minimal bactericidal concentration

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Acknowledgments

This study was supported by grants from the Jagiellonian University Medical College (grant no. K/ZDS/005454).

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Authors and Affiliations

  1. Chair of Immunology, Jagiellonian University Medical College, Krakow, Poland

    Angelika Peruń, Marta Ciszek-Lenda, Maria Walczewska, Aneta Kiecka & Janusz Marcinkiewicz

  2. Center of Microbiological Research and Autovaccines Ltd., Krakow, Poland

    Anna Białecka

  3. Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria

    Markus Nagl & Waldemar Gottardi

Authors
  1. Angelika Peruń
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  2. Marta Ciszek-Lenda
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  3. Maria Walczewska
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  4. Aneta Kiecka
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  5. Anna Białecka
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  6. Markus Nagl
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  7. Waldemar Gottardi
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  8. Janusz Marcinkiewicz
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Corresponding author

Correspondence to Janusz Marcinkiewicz .

Editor information

Editors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, China

    Jianmin Hu

  2. Department of Occupational and Environmental Health, Dalian Medicine University, Dalian, Liaoning, China

    Fengyuan Piao

  3. Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL, USA

    Stephen W. Schaffer

  4. Department of Biology, Center for Developmental Neuroscience, College of Staten Island, Staten Island, NY, USA

    Abdeslem El Idrissi

  5. Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, USA

    Jang-Yen Wu

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Peruń, A. et al. (2019). Combined Biological Effects of N-Bromotaurine Analogs and Ibuprofen. Part II: Influence on a Local Defense System. In: Hu, J., Piao, F., Schaffer, S., El Idrissi, A., Wu, JY. (eds) Taurine 11. Advances in Experimental Medicine and Biology, vol 1155. Springer, Singapore. https://doi.org/10.1007/978-981-13-8023-5_85

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