Abstract
Atrina pectinata (A. pectinata), called pen shell, is an edible shellfish that adheres to the seabed pointed downward and has a triangular shell reaching about 40 cm in length.
In this study, we examined the antioxidant effect of an A. pectinata extract exhibiting various radical scavenging activities. These scavenging activities were evaluated using electron spin resonance. Anti-oxidant activities were also determined using the ferric reducing antioxidant power (FRAP) and the ABTS radical scavenging assays. Lipid peroxidation inhibitory activity was confirmed using ferric thiocyanate and thiobarbituric acid assays. Furthermore, the protective effect of the A. pectinata extract against t-BHP-induced oxidative stress on Chang cells were evaluated using MTT assay and the measurement of reactive oxygen species (ROS). These results showed that the A. pectinata extract have strong radical scavenging activities, and exerts protective effect against oxidative stress through reducing intracellular ROS content of Chang cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “Antioxidant Power”: the FRAP assay. Anal Biochem 239:70–76
Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O (2012) Oxidative stress and antioxidant defense. World Allergy Organ J 5:9–19
Capecka E, Mareczek A, Leja M (2005) Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chem 93(2):223–226
Chen LB (1988) Mitochondrial membrane potential in living cells. Annu Rev Cell Biol 4:155–181
Dávalos A, Gómez-Cordovés C, Bartolome B (2004) Extending applicability of the oxygen radical absorbance capacity (ORAC-fluorescein) assay. J Agric Food Chem 52(1):48–54
Dhalla NS, Temsah RM, Netticadan T (2000) Role of oxidative stress in cardiovascular diseases. J Hypertens 18:655–673
FAO (1998) FAO species identification guide for fishery purpose. The living marine resources of the Western Central Pacific, vol 1. Seaweeds, Corals, Bivalves and Gastropods
Gordon RE, Park E, Laskin D, Schuller-Levis GB (1998) Taurine protects rat bronchioles from acute ozone exposure: a freeze fracture and electron microscopic study. Exp Lung Res 24:659–674
Guo H, Saravanakumar K, Wang MH (2018) Total phenolic, flavonoid contents and free radical scavenging capacity of extracts from tubers of Stachys affinis. Biocatal Agric Biotechnol 15:235–239
Kasparova S, Brezova V, Valko M, Horecky J, Mlynarik V et al (2005) Study of the oxidative stress in a rat model of chronic brain hypoperfusion. Neurochem Int 46:601–611
Kerr S, Brosnan MJ, McIntyre M, Reid JL, Dominiczak AF, Hamilton CA (1999) Superoxide anion production is increased in a model of genetic hypertension: role of the endothelium. Hypertension 33:1353–1358
Loft S, Poulsen HE (1998) Markers of oxidative damage to DNA: antioxidants and molecular damage. Methods Enzymol 300:166–184
Luo X, Zhang H, Duan Y, Chen G (2018) Protective effects of radish (Raphanus sativus L.) leaves extract against hydrogen peroxide-induced oxidative damage in human fetal lung fibroblast (MRC-5) cells. Biomed Pharmacother 103:406–414
Okutani T (1997) Basic data on scarcely wild species in Japan–IV. Jpn Fish Resour Conserv Assoc Tokyo: 43–47
Prior RL, Hoang H, Gu L, Wu X, Bacchiocca M, Howard L, Hampsch-Woodill M, Huang D, Ou B, Jacob R (2003) Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORAC(FL))) of plasma and other biological and food samples. J Agric Food Chem 51:3273–3279
Prior RL, Wu X, Schaich K (2005) Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53:4290–4302
Rahman (2007) Studies on free radicals, antioxidants, and co-factors. Clin Interv Aging 2:219–236
Redza-Dutordoir M, Averill-Bates DA (2016) Activation of apoptosis signalling pathways by reactive oxygen species. Biochim Biophys Acta (BBA) – Mol Cell Res 1863(12):2977–2992
Satoh T, Enokido Y, Aoshima H, Uchiyama Y, Hatanaka H (1997) Changes in mitochondrial membrane potential during oxidative stress-induced apoptosis in PC12 cells. J Neurosci Res 50:413–420
Stapleton PP, O’Flaherty L, Redmond HP, Bouchier-Hayes DJ (1998) Review: host defense-a role for the amino acid taurine? J Parenter Enter Nutr 22:42–48
Tai A, Sawana T, Yazama F (2011) Antioxidant properties of ethyl vanillin in vitro and in vivo. Biosci Biotechnol Biochem 75(12):2346–2350
Timbrell JA, Seabra V, Waterfield CJ (1995) The in vivo and in vitro protective properties of taurine. Gen Pharmacol Vasc Syst 26:453–462
Trujillo J, Chirino YI, Molina-Jijón E, Andérica-Romero AC, Tapia E, Pedraza-Chaverrí J (2013) Renoprotective effect of the antioxidant curcumin: recent findings. Redox Biol 1(1):448–456
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160:1–40
Venkatachalam S, Kuppusamy P, Kuppusamy B, Dhanapal S (2014) The potency of essential nutrient taurine on boosting the antioxidant status and chemopreventive effect against benzo (a) pyrene induced experimental lung cancer. Biomed Prev Nutr 4:251–255
Venugopal V, Gopakumar K (2017) Shellfish: nutritive value, health benefits, and consumer safety. Compr Rev Food Sci Food Saf 16(6):1219–1242
Wang MY, Dhingra K, Hittelman WN, Liehr JG, deAndrade M, Li DH (1996) Lipid peroxidation-induced putative malondialdehyde–DNA adducts in human breast tissues. Cancer Epidemiol Biomark Prev 6(5):705–710
Waris G, Ahsan H (2006) Reactive oxygen species: role in the development of cancer and various chronic conditions. J Carcinog 5:14
Wu X, Gu L, Holden J, Haytowitz DB, Gebhardt SE, Beecher G, Prior RL (2004) Development of a database for total antioxidant capacity in foods: a preliminary study. J Food Compos Anal 17:407–422
Yurimoto Y, Watanabe Y, Nasu H, Tobase N, Matsui S, Yoshioka N (2003) Relationship between environmental food and glycogen contents in pen shells. In: Proceedings of 32nd US Japan Symposium on Aquaculture, pp 16–23. California, USA
Zitka O, Skalickova S, Gumulec J, Masarik M, Adam V, Hubalek J, Trnkova L, Kruseova J, Eckschlager T, Kizek R (2012) Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients. Oncol Lett 4(6):1247–1253
Conflict of Interest
The authors declare that there are no conflicts of interest.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
Kim, YS. et al. (2019). Antioxidant and Protective Effects of Atrina Pectinata Extract. In: Hu, J., Piao, F., Schaffer, S., El Idrissi, A., Wu, JY. (eds) Taurine 11. Advances in Experimental Medicine and Biology, vol 1155. Springer, Singapore. https://doi.org/10.1007/978-981-13-8023-5_56
Download citation
DOI: https://doi.org/10.1007/978-981-13-8023-5_56
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-13-8022-8
Online ISBN: 978-981-13-8023-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)