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Taurine Increases Spermatozoa Quality and Function in Asthenospermia Rats Impaired by Ornidazole

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Taurine 11

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1155))

Abstract

Asthenospermia has been considered as one of the crucial causes of male infertility, which was closely related to epididymal dysfunction. Lots of documents have revealed that taurine palys an important role in male reproduction, including antioxidation, membrane stabilization, stimulation of sexual hormone secretion and elevation of sperm quality. The objective of this study was to expose the effect of taurine on spermatozoa quality and function in ornidazole-induced asthenospermia rats. We found that taurine treatment could obviously recover the decline of cauda epididymal sperm count, viability and motility, and the elevation of sperm abnormality in asthenospermia animals. Spermatozoa acrosin, LDH-X, SDH and CCO activities of model rats also were notably increased by taurine administration. The present data indicated that taurine could raise spermatozoa quality and function by elevating mitochondrial energy metabolism. Notably, taurine supplementation markedly raised serum GnRH, LH and T levels in asthenospermia rays, suggesting taurine rescued asthenosperm by means of stimulating hypothalamic-pituitary-testicular axis secretion. We also found that concentrations of asthenospermia epididymal carnitine, SA, α-Glu and ACP, and mRNA expression levels of MMP7 and IDO2 were significantly rised by taurine administration, indicating taurine may protect epididymal epithelium structure, improve secretion activity, and maintain intraluminal microenvironment homeostasis. Finally, the present results showed taurine effectively increased cauda epididymal SOD, GSH and γ-GT levels in model rats, reduced ROS and MDA production, suggesting epididymal antioxidant ability of asthenospermia rats could be elevated by taurine treatment. To sum up, our results indicated that taurine can promote spermatozoa quality and function in ornidazole-induced asthenospermia rats by facilitating epididymal epithelium secretion and luminal microenvironment homeostasis.

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Abbreviations

ACP:

acid phosphatase

CCO:

cytochrome c oxidase

FSH:

follicle-stimulating hormone

GnRH:

gonadotropin-releasing hormone

GSH:

reduced glutathione hormone

IDO2:

indoleamine 2, 3-dioxygenase

LDH-X:

lactate dehydrogenase X

LH:

luteinizing hormone

MDA:

malondialdehyde

MMP7:

matrix metalloproteinase 7

ROS:

reactive oxygen species

SA:

sialic acid

SDH:

sorbitol dehydrogenase

SOD:

superoxide dismutase

T:

testosterone

α-Glu:

α-glucosidase

γ-GT:

γ-glutamyl-transferase

References

  • Abdulaziz M, Meriano J, Casper RF (1996) Intracytoplasmic sperm injection for treatment of infertility due to acrosomal enzyme deficiency. Fertil Steril 65(5):977–980

    Article  CAS  Google Scholar 

  • Adedara IA, Alake SE, Adeyemo MO, Olajide LO, Ajibade TO, Farombi EO (2017) Taurine enhances spermatogenic function and antioxidant defense mechanisms in testes and epididymis of L-NAME-induced hypertensive rats. Biomed Pharmacother 97:181

    Article  Google Scholar 

  • Agarwal A, Ikemoto I, Loughlin KR (1994) Relationship of sperm parameters with levels of reactive oxygen species in semen specimens. J Urol 152(1):107–110

    Article  CAS  Google Scholar 

  • Agarwal A, Sharma RK, Sharma R, Assidi M, Abuzenadah AM, Alshahrani S, Durairajanayagam D, Sabanegh E (2014a) Characterizing semen parameters and their association with reactive oxygen species in infertile men. Reprod Biol Endocrinol 12:33

    Article  Google Scholar 

  • Agarwal A, Virk G, Ong C, Plessis SSD (2014b) Effect of oxidative stress on male reproduction. World J Mens Health 32(1):1–17

    Article  Google Scholar 

  • Aitken RJ (1995) Free radicals, lipid peroxidation and sperm function. Reprod Fertil Dev 7(4):659–668

    Article  CAS  Google Scholar 

  • Ajina T, Sallem A, Haouas Z, Mehdi M (2017) Total antioxidant status and lipid peroxidation with and without in vitro zinc supplementation in infertile men. Andrologia 49(7):1–5

    Article  Google Scholar 

  • Alam SS, Hafiz NA, El-Rahim AHA (2011) Protective role of taurine against genotoxic damage in mice treated with methotrexate and tamoxfine. Environ Toxicol Pharmacol 31(1):143–152

    Article  CAS  Google Scholar 

  • Alvarez JG, Storey BT (1983) Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biol Reprod 29(3):548–555

    Article  CAS  Google Scholar 

  • Balkan J, Kanbağli O, Hatipoğlu A, Kücük M, Cevikbaş U, Aykaçtoker G, Uysal M (2002) Improving effect of dietary taurine supplementation on the oxidative stress and lipid levels in the plasma, liver and aorta of rabbits fed on a high-cholesterol diet. Biosci Biotechnol Biochem 66(8):1755–1758

    Article  CAS  Google Scholar 

  • Cozzi R, Ricordy R, Bartolini F, Ramadori L, Perticone P, De SR (2010) Taurine and ellagic acid: two differently-acting natural antioxidants. Environ Mol Mutagen 26(3):248–254

    Article  Google Scholar 

  • Curi SM, Ariagno JI, Chenlo PH, Mendeluk GR, Pugliese MN, Sardi Segovia LM, Repetto HE, Blanco AM (2003) Asthenozoospermia: analysis of a large population. Arch Androl 49(5):343–349

    Article  CAS  Google Scholar 

  • Dogru-Abbasoglu S, Kanbagli O, Balkan J, Cevikbas U, Aykac-Toker G, Uysal M (2001) The protective effect of taurine against thioacetamide hepatotoxicity of rats. Hum Exp Toxicol 20(1):23–27

    Article  CAS  Google Scholar 

  • Engström L, Stenberg M, Aronsson H, Lindén B (1985) Inhibition of hamster sperm Na+, K+-ATPase activity by taurine and hypotaurine. Life Sci 36(3):271–275

    Article  Google Scholar 

  • Forti G, Krausz C (1998) Clinical review 100: evaluation and treatment of the infertile couple. J Clin Endocrinol Metab 83(12):4177–4188

    CAS  PubMed  Google Scholar 

  • Gharagozloo P, Aitken RJ (2011) The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy. Hum Reprod 26(7):1628–1640

    Article  Google Scholar 

  • Higuchi M, Celino FT, Tamai A, Miura C, Miura T (2012) The synthesis and role of taurine in the Japanese eel testis. Amino Acids 43(2):773–781

    Article  CAS  Google Scholar 

  • Hinton BT (1990) The testicular and epididymal luminal amino acid microenvironment in the rat. J Androl 11(6):498–505

    CAS  PubMed  Google Scholar 

  • Holmes RP, Goodman HO, Shihabi ZK, Jarow JP (1992) The taurine and hypotaurine content of human semen. J Androl 13(3):289–292

    CAS  PubMed  Google Scholar 

  • Irvine DS (1998) Epidemiology and aetiology of male infertility. Hum Reprod 13(Suppl 1):33–44

    Article  Google Scholar 

  • Iwasaki A, Gagnon C (1992) Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril 57(2):409–416

    Article  CAS  Google Scholar 

  • Jeulin C, Dacheux JL, Soufir JC (1994) Uptake and release of free L-carnitine by boar epididymal spermatozoa in vitro and subsequent acetylation rate. J Reprod Fertil 100(1):263

    Article  CAS  Google Scholar 

  • Kobori Y, Ota S, Sato R, Yagi H, Soh S, Arai G, Okada H (2014) Antioxidant cosupplementation therapy with vitamin C, vitamin E, and coenzyme Q10 in patients with oligoasthenozoospermia. Arch Ital Urol Androl 86(1):1–4

    Article  Google Scholar 

  • Koyama I, Nakamura T, Ogasawara M, Nemoto M, Yoshida T (1992) The protective effect of taurine on the biomembrane against damage produced by the oxygen radical. Adv Exp Med Biol 315:355–359

    Article  CAS  Google Scholar 

  • Krausz C (2011) Male infertility: pathogenesis and clinical diagnosis. Best Pract Res Clin Endocrinol Metab 25(2):271–285

    Article  Google Scholar 

  • Li JH, Ling YQ, Fan JJ, Zhang XP, Cui S (2006) Expression of cysteine sulfinate decarboxylase (CSD) in male reproductive organs of mice. Histochem Cell Biol 125(6):607–613

    Article  CAS  Google Scholar 

  • Linder RE, Klinefelter GR, Strader LF, Narotsky MG, Suarez JD, Roberts NL, Perreault SD (1995) Dibromoacetic acid affects reproductive competence and sperm quality in the male rat. Fundamappltoxicol 28(1):9

    CAS  Google Scholar 

  • Liu H, Lin S, Lv Q, Yang Q, Wu G, Hu J, Yang J (2017a) Taurine recovers testicular steroidogenesis and spermatogenesis in streptozotocin-induced diabetic rats. Adv Exp Med Biol 975:801–811

    Article  CAS  Google Scholar 

  • Liu W, Kang XF, Zhang GW, Cai HC, Li KQ, Wang LL, Shang XJ (2017b) Protective effect of astaxanthin against epididymal oxidative damagein rats with ornidazole-induced oligoasthenozoospermia. Natl J Androl 23(3):206–211

    Google Scholar 

  • Mcclain RM, Downing JC (1988) The effect of ornidazole on fertility and epididymal sperm function in rats. Toxicol Appl Pharmacol 92(3):488–496

    Article  CAS  Google Scholar 

  • Meizel S, Lui CW, Working PK, Mrsny RJ (1980) Taurine and hypotaurine: their effects on motility, capacitation and the acrosome reaction of hamster sperm in vitro and their presence in sperm and reproductive tract fluids of several mammals. Dev Growth Differ 22(3):483–494

    Article  CAS  Google Scholar 

  • Miki K (2007) Energy metabolism and sperm function. Soc Reprod Fertil Suppl 65:309–325

    CAS  PubMed  Google Scholar 

  • Murakami S, Ono A, Kawasaki A, Takenaga T, Ito T (2018) Taurine attenuates the development of hepatic steatosis through the inhibition of oxidative stress in a model of nonalcoholic fatty liver disease in vivo and in vitro. Amino Acids 50(9):1279–1288

    Article  CAS  Google Scholar 

  • Oberländer G, Yeung CH, Cooper TG (1996) Influence of oral administration of ornidazole on capacitation and the activity of some glycolytic enzymes of rat spermatozoa. J Reprod Fertil 106(2):231–239

    Article  Google Scholar 

  • Pang XB, Zhu Y, Li HG, Zhou H, Zhu JW, Liao AH, Chang CF (2005) Effect of ornidazole on sperm in rats and its mechanism of action. Zhonghua Nan Ke Xue 11(1):26–28

    CAS  PubMed  Google Scholar 

  • Purvis K, Brekke I, Tollefsrud A (1991) Epididymal secretory function in men with asthenoteratozoospermia. Hum Reprod 6(6):850–853

    Article  CAS  Google Scholar 

  • Robaire B, Hamzeh M (2011) Androgen action in the epididymis. J Androl 32(6):592–599

    Article  CAS  Google Scholar 

  • Ruiz-Pesini E, Alvarez E, Enríquez JA, López-Pérez MJ (2001) Association between seminal plasma carnitine and sperm mitochondrial enzymatic activities. Int J Androl 24(6):335–340

    Article  CAS  Google Scholar 

  • Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 3(6):1101–1108

    Article  CAS  Google Scholar 

  • Shimada K, Jong CJ, Schaffer SW, Shimada K, Takahashi K, Takahashi K (2015) Role of ROS production and turnover in the antioxidant activity of taurine. Adv Exp Med Biol 803:581–596

    Article  CAS  Google Scholar 

  • Sun M, Gu Y, Zhao Y, Xu C (2011) Protective functions of taurine against experimental stroke through depressing mitochondria-mediated cell death in rats. Amino Acids 40(5):1419–1429

    Article  CAS  Google Scholar 

  • Suzuki T, Nagao A, Suzuki T (2011) Human mitochondrial diseases caused by lack of taurine modification in mitochondrial tRNAs. Wiley Interdiscip Rev RNA 2(3):376–386

    Article  CAS  Google Scholar 

  • Timbrell JA, Seabra V, Waterfield CJ (1995) The in vivo and in vitro protective properties of taurine. Gen Pharmacol 26(3):453–462

    Article  CAS  Google Scholar 

  • Yan F, Chen Y, Zuo Z, Chen Y, Yang Z, Wang C (2009) Effects of tributyltin on epididymal function and sperm maturation in mice. Environ Toxicol Pharmacol 28(1):19–24

    Article  CAS  Google Scholar 

  • Yang J, Wu G, Feng Y, Lv Q, Lin S, Hu J (2010a) Effects of taurine on male reproduction in rats of different ages. J Biomed Sci 17(Suppl 1):S9

    Article  Google Scholar 

  • Yang J, Wu G, Feng Y, Sun C, Lin S, Hu J (2010b) CSD mRNA expression in rat testis and the effect of taurine on testosterone secretion. Amino Acids 39(1):155–160

    Article  CAS  Google Scholar 

  • Yang J, Zong X, Wu G, Lin S, Feng Y, Hu J (2015) Taurine increases testicular function in aged rats by inhibiting oxidative stress and apoptosis. Amino Acids 47(8):1549–1558

    Article  CAS  Google Scholar 

  • Yoshida R, Nukiwa T, Watanabe Y, Fujiwara M, Hirata F, Hayaishi O (1980) Regulation of indoleamine 2,3-dioxygenase activity in the small intestine and the epididymis of mice. Arch Biochem Biophys 203(1):343–351

    Article  CAS  Google Scholar 

  • Zhang W, Liu QZ, Shang XJ, Huang YF, Wang HY (2009) L-carnitine improves the reproductive function of male rats with ornidazole-induced asthenospermia. Natl J Androl 15(7):604

    Google Scholar 

  • Zhang WD, Zhang Z, Jia LT, Zhang LL, Fu T, Li YS, Wang P, Sun L, Shi Y, Zhang HZ (2014) Oxygen free radicals and mitochondrial signaling in oligospermia and asthenospermia. Mol Med Rep 10(4):1875–1880

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No.31272522) and Program for Liaoning Excellent Talents in University (No. LJQ2014073).

Author Contributions

JY and JH conceived the study and participated in its design and coordination and helped to draft the manuscript. TM performed the sexual function tests, participated in the study design and drafted the manuscript. SL and GW performed the hormone study. QY and YZ participated in the detection of NOS/NO and performed the statistical analysis. All authors read and approved the final manuscript.

Conflict of Interest

All authors declare no conflicts of interest, financial or otherwise.

Author information

Author notes

  1. Yanting Du and Hong Liu contributed equally with all other contributors.

Authors and Affiliations

  1. Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, China

    Yanting Du, Meng Zhang, Shu Zhang, Jianmin Hu, Gaofeng Wu & Jiancheng Yang

  2. Experimental Animal Center, Liaoning University of Traditional Chinese Medicine, Shenyang, China

    Hong Liu

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  1. Yanting Du
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Editor information

Editors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, China

    Jianmin Hu

  2. Department of Occupational and Environmental Health, Dalian Medicine University, Dalian, Liaoning, China

    Fengyuan Piao

  3. Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL, USA

    Stephen W. Schaffer

  4. Department of Biology, Center for Developmental Neuroscience, College of Staten Island, Staten Island, NY, USA

    Abdeslem El Idrissi

  5. Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, USA

    Jang-Yen Wu

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Du, Y. et al. (2019). Taurine Increases Spermatozoa Quality and Function in Asthenospermia Rats Impaired by Ornidazole. In: Hu, J., Piao, F., Schaffer, S., El Idrissi, A., Wu, JY. (eds) Taurine 11. Advances in Experimental Medicine and Biology, vol 1155. Springer, Singapore. https://doi.org/10.1007/978-981-13-8023-5_47

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