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Effect of Taurine on Cell Function via TXNIP Induction in Caco-2 Cells

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Taurine 11

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1155))

Abstract

Taurine (2-aminoethanesulfonic acid), a sulfur-containing β-amino acid, is a free amino acid present in high concentrations in mammalian tissues. Taurine has pivotal roles in anti-oxidation, membrane stabilization, osmoregulation, anti-inflammation, and other process. In a DNA microarray analysis, we previously found that taurine markedly increases the mRNA expression of thioredoxin interacting protein (TXNIP) in Caco-2 cells. In this study, we investigated the effect of these taurine-induced changes in TXNIP on the function of Caco-2 cells. We found that taurine decreases glucose uptake in a dose-dependent manner. The taurine-induced decrease in glucose uptake was completely abolished by transfection with siRNA against TXNIP, suggesting that TXNIP is involved in the taurine-induced down-regulation of glucose uptake. We also revealed that taurine induces AMPK activation and further increases the intracellular ATP content in Caco-2 cells. These results suggest that taurine could regulate the function of Caco-2 cells via TXNIP induction, leading to extend our understanding of the functions of taurine.

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Abbreviations

TXNIP :

thioredoxin interacting protein

AMP :

adenosine monophosphate

AMPK :

adenosine monophosphate kinase

ATP :

adenosine triphosphate

GLUT1 :

glucose transporter 1

GLUT3 :

glucose transporter 3

ChoRE :

carbohydrate response element

ChREBP :

carbohydrate response element binding protein

mTOR :

mammalian target of rapamycin

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Author information

Authors and Affiliations

  1. Department of Biotechnology, Maebashi Institute of Technology, Maebashi, Japan

    Hideo Satsu, Kenji Watari & Midori Fukumura

  2. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

    Yusuke Gondo & Hana Shimanaka

  3. Department of Nutritional Science, Tokyo University of Agriculture, Tokyo, Japan

    Makoto Shimizu

Authors
  1. Hideo Satsu
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  2. Yusuke Gondo
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  3. Hana Shimanaka
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  4. Kenji Watari
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  5. Midori Fukumura
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  6. Makoto Shimizu
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Corresponding author

Correspondence to Hideo Satsu .

Editor information

Editors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, China

    Jianmin Hu

  2. Department of Occupational and Environmental Health, Dalian Medicine University, Dalian, Liaoning, China

    Fengyuan Piao

  3. Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL, USA

    Stephen W. Schaffer

  4. Department of Biology, Center for Developmental Neuroscience, College of Staten Island, Staten Island, NY, USA

    Abdeslem El Idrissi

  5. Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, USA

    Jang-Yen Wu

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© 2019 Springer Nature Singapore Pte Ltd.

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Satsu, H., Gondo, Y., Shimanaka, H., Watari, K., Fukumura, M., Shimizu, M. (2019). Effect of Taurine on Cell Function via TXNIP Induction in Caco-2 Cells. In: Hu, J., Piao, F., Schaffer, S., El Idrissi, A., Wu, JY. (eds) Taurine 11. Advances in Experimental Medicine and Biology, vol 1155. Springer, Singapore. https://doi.org/10.1007/978-981-13-8023-5_15

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