Abstract
Tissue culture is the in vitro aseptic (sterile) culture of cells, tissues and organs under controlled nutritional and environmental conditions. Two concepts, plasticity and totipotency (ability of a cell to give rise to new organism or part), are central to understanding plant tissue culture. It involves the use of small pieces of plant tissue (explants) which are cultured in a nutrient medium under sterile conditions. Using the appropriate growing conditions for each explant type, tissues can be induced to rapidly produce new shoots and roots. These plantlets can also be divided, usually at the shoot stage, to produce large numbers of new plantlets. The new plants can then be placed in soil and grown in the normal way.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Further Reading
Anis M, Ahmad N (2016) Plant tissue culture: propagation, conservation and crop improvement. Springer, Singapore
Bhojwani SS, Grover A (1996) Tissue culture a novel source of genetic variations. Botanica 46:1–6
Davey MR, Anthony P (2010) Plant cell culture: essential methods. Wiley-Blackwell, Hoboken
Dodds J (2004) Experiments in plant tissue culture. Cambridge University Press, Cambridge
George EF (1993) Plant propagation by tissue culture. In: Part 1, The Technology. Edington, Exegetics Ltd
Gray DJ, Purohit A, Triglano RN (1991) Somatic embryogenesis and development of synthetic seed technology. Crit Rev Plant Sci 10:33–61
Iliev et al. (2010) Plant micropropagation. In: Davey and Anthony P (eds.) Plant cell culture. Wiley
Kyte et al (2013) Plants from test tubes: an introduction to micropropagation. Timber press, Portland
Murashige T (1974) Plant propagation through tissue culture. Ann Rev Plant Physiol 25:135–166
Onishi N, Sakamoto Y, Hirosawa T (1994) Synthetic seeds as an application of mass-production of somatic embryos. Plant Cell Tissue Organ Cult 39:137–145
Redenbaugh K, Fujii JA, Slade D (1988) Encapsulated plant embryos. In: Mizrahi A (ed) Advances in biotechnological processes. Alan R. Liss Inc., New York
Rihan HZ et al (2017) Artificial seeds (Principle, aspects and applications). Agronomy 7:71. https://doi.org/10.3390/agronomy7040071
Sathyanarayana BN (2007) Plant tissue culture: practices and new experimental protocols. New Delhi, I. K. International
Shahzad A et al (2017) Historical perspective and basic principles of plant tissue culture. In: Plant biotechnology: principles and applications. Springer, pp 1–36
Smith R (2012) Plant tissue culture 3rd Edn. Techniques and experiments. Elsevier, Amsterdam
Trigiano RN, Gray DJ (2010) Plant tissue culture, development, and biotechnology. Taylor and Francis. https://doi.org/10.1201/9781439896143
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Priyadarshan, P.M. (2019). Tissue Culture. In: PLANT BREEDING: Classical to Modern. Springer, Singapore. https://doi.org/10.1007/978-981-13-7095-3_21
Download citation
DOI: https://doi.org/10.1007/978-981-13-7095-3_21
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-13-7094-6
Online ISBN: 978-981-13-7095-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)