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Phytopathogen Biomass as Inducer of Antifungal Compounds by Trichoderma asperellum Under Solid-State Fermentation

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Secondary Metabolites of Plant Growth Promoting Rhizomicroorganisms

Abstract

The main objective of the present study was to evaluate the phytopathogen biomass of Phytophthora capsici and Colletotrichum gloeosporioides as inducers of antifungal metabolites from Trichoderma asperellum. The experiment was carried out through a solid-state fermentation using corncob as support/substrate. Water, ethanol, and toluene were used to recover the antifungal metabolites. The strain of P. capsici was inhibited by the metabolites recovered from all extracts; however C. gloeosporioides resist them and develop a normal growth. The bioactive extracts were fractioned using Amberlite XAD16®, and each fraction was analyzed by LC-ESI-MS. LC-ESI-MS analysis showed two major compounds; an unknown compound (1) was detected as [M + H]− (m/z 478) while dihydroxybergamotene (2) as [M + H]− (m/z 260). In addition, other four compounds were detected: viridepyronone (3), koninginin D (4), acetyltetrahydroxyanthraquinone (5), and virone or gliotoxin (6). The results suggest that the biomass of P. capsici worked as an inducer of antibiotic compounds in T. asperellum.

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Authors and Affiliations

  1. Department of Food Research, School of Chemistry, Universidad Autónoma de Coahuila (UAdeC), Saltillo, Mexico

    Reynaldo De la Cruz-Quiroz, Juan Alberto Ascacio-Valdés, Raúl Rodríguez-Herrera & Cristóbal N. Aguilar

  2. Institut Méditerranéen de Biodiversité et d’Ecologie Marine et Continentale (IMBE), Aix Marseille Université, Marseille Cedex 20, France

    Sevastianos Roussos

  3. Faculté des sciences St Jérôme, University of Avignon, CNRS, IRD, Avignon, France

    Sevastianos Roussos

Authors
  1. Reynaldo De la Cruz-Quiroz
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  2. Juan Alberto Ascacio-Valdés
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  3. Raúl Rodríguez-Herrera
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  4. Sevastianos Roussos
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  5. Cristóbal N. Aguilar
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Corresponding author

Correspondence to Cristóbal N. Aguilar .

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Editors and Affiliations

  1. Department of Mycology and Plant Pathology, Banaras Hindu University, Varanasi, Uttar Pradesh, India

    Harikesh Bahadur Singh

  2. Department of Biochemistry, Banaras Hindu University, Varanasi, Uttar Pradesh, India

    Chetan Keswani

  3. Department of Entomology & Plant Pathology, Auburn University, Auburn, AL, USA

    M. S. Reddy

  4. Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Pue, Puebla, Mexico

    Estibaliz Sansinenea

  5. Instituto de Biotecnología de León, (INBIOTEC), León, León, Spain

    Carlos García-Estrada

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De la Cruz-Quiroz, R., Ascacio-Valdés, J.A., Rodríguez-Herrera, R., Roussos, S., Aguilar, C.N. (2019). Phytopathogen Biomass as Inducer of Antifungal Compounds by Trichoderma asperellum Under Solid-State Fermentation. In: Singh, H., Keswani, C., Reddy, M., Sansinenea, E., García-Estrada, C. (eds) Secondary Metabolites of Plant Growth Promoting Rhizomicroorganisms. Springer, Singapore. https://doi.org/10.1007/978-981-13-5862-3_6

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