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Status of Membrane Asymmetry in Erythrocytes: Role of Spectrin

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Biochemical and Biophysical Roles of Cell Surface Molecules

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1112))

Abstract

Spectrin-based proteinaceous membrane skeletal network has been found to be implicated in membrane disorders like hereditary spherocytosis (HS). HS greatly affects eryptosis via loss of membrane asymmetry which is seen to be the case in haemoglobin disorders like thalassemia and sickle cell disease as well. The biological implications of the status of membrane asymmetry are strongly correlated to spectrin interactions with aminophospholipids, e.g. PE and PS. Fluorescence and X-ray reflectivity (XRR) measurements of spectrin interactions with small unilamellar vesicles (SUVs) and cushioned bilayers of phospholipids, respectively, were studied. Both the XRR and fluorescence measurements led to the characterization of spectrin orientation on the surface of lipid bilayer of phosphatidylcholine (PC) and PC/aminophospholipid mixed membrane systems showing formation of a uniform layer of spectrin on top of the mixed phospholipid bilayer. Fluorescence studies show that spectrin interacts with PC and phosphatidylethanolamine (PE)/phosphatidylserine (PS) membranes with binding dissociation constants (Kd) in the nanomolar range indicating the role of spectrin in the maintenance of the overall membrane asymmetry of erythrocytes.

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Abbreviations

DM:

Dimyristoyl

HE:

Hereditary elliptocytosis

HS:

Hereditary spherocytosis

PC:

Phosphatidylcholine

PE:

Phosphatidylethanolamine

PG:

Phosphoglycerate

PI:

Phosphatidylinositol

PS:

Phosphatidylserine

SUV:

Small unilamellar vesicles

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Author information

Authors and Affiliations

  1. Crystallography & Molecular Biology Division, Saha Institute of Nuclear Physics, HBNI, Kolkata, India

    Sauvik Sarkar, Dipayan Bose & Abhijit Chakrabarti

  2. Surface Physics & Material Science Division, Saha Institute of Nuclear Physics, HBNI, Kolkata, India

    Rajendra P. Giri & Mrinmay K. Mukhopadhyay

Authors
  1. Sauvik Sarkar
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  2. Dipayan Bose
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  3. Rajendra P. Giri
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  4. Mrinmay K. Mukhopadhyay
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  5. Abhijit Chakrabarti
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Corresponding author

Correspondence to Abhijit Chakrabarti .

Editor information

Editors and Affiliations

  1. Centre for Protein Science, Design and Engineering, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Mohali, Punjab, India

    Kausik Chattopadhyay

  2. Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA

    Subhash C. Basu

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Sarkar, S., Bose, D., Giri, R.P., Mukhopadhyay, M.K., Chakrabarti, A. (2018). Status of Membrane Asymmetry in Erythrocytes: Role of Spectrin. In: Chattopadhyay, K., Basu, S. (eds) Biochemical and Biophysical Roles of Cell Surface Molecules. Advances in Experimental Medicine and Biology, vol 1112. Springer, Singapore. https://doi.org/10.1007/978-981-13-3065-0_1

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