Abstract
In the past three decades, differentiated hairy root culture-related researches gained a great attention due to the equal or greater bio-production capacity of low amount, high-value secondary metabolites as compared to their parent plants with several advantages over undifferentiated cell suspension cultures in plants. This was mainly because hairy roots are capable of auxin-independent rapid growth and are genetically and biochemically stable, with high productivity and suitability for adaptation to large-scale systems. Nowadays, hairy root cultures of various plant species offer a novel promising opportunity and great prospects for in vitro mass production of economically important bioactive metabolites. At present, the productivity of desired compounds by hairy root cultures is generally too low to fulfill the demands of pharmaceutical industry owing to various biological and technological limitations. Screening and selection for high-yielding root lines and optimization of the culture media and the culture conditions like type of nutrient medium, salt strength, source of carbon and concentration, source of nitrogen and the ratio of NH4 +/NO3 −, concentration of phosphate, inoculum density, hydrogen ion concentration, temperature, and light intensity and quality have been taken as yield enhancement strategies among others, to produce desired secondary metabolites using hairy root cultures. Feasibility of commercial application of hairy root culture in bioreactors requires several optimization steps. This review highlights some of the recent progress and outlines future prospects for metabolite production and yield enhancement approaches in hairy root cultures for producing bioactive substances.
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References
Abbasi BH, Stiles AR, Saxena PK, Liu CZ (2012) Gibberellic acid increases secondary metabolite production in Echinacea purpurea hairy roots. Appl Biochem Biotechnol 168(7):2057–2066
Almagro L, Belchí-Navarro S, Sabater-Jara AB, Vera-Urbina JC, Sellés-Marchart S, Bru R, Pedreño MA (2013) Bioproduction of trans-resveratrol from grapevine cell cultures. In: Ramawat KG, Merillon JM (eds) Handbook of natural products. Springer, Berlin, pp 1683–1713
Amdoun R, Khelifi L, Khelifi-Slaoui M, Amroune S, Asch M, Assaf-Ducrocq C, Gontier E (2010) Optimization of the culture medium composition to improve the production of hyoscyamine in elicited Datura stramonium L. hairy roots using the response surface methodology (RSM). Int J Mol Sci 11(11):4726–4740
Anderson WC (1978) Tissue culture propagation of rhododendrons. In Vitro 14:334
Anderson WC (1980) Tissue culture propagation of red and black raspberries, Rubus idaeus and R. occidentalis. Acta Hortic 112:13–20
Ashraf MF, Zain CRCM, Zainal Z, Noor NM, Anuar N, Markom M, Ismail I (2015) Establishment of Persicaria minor hairy roots and analysis of secreted β-caryophyllene in medium broth. Plant Cell Tiss Organ Cult 121(1):11–20
Bakkali AT, Jaziri M, Foriers A, Vander Heyden Y, Vanhaelen M, Homes J (1997) Lawsone accumulation in normal and transformed cultures of henna, Lawsonia inermis. Plant Cell Tiss Organ Cult 51(2):83–87
Banerjee S, Singh S, Rahman LU (2012) Biotransformation studies using hairy root cultures- a review. Biotechnol Adv 30(3):461–468
Basu A, Joshi RK, Jha S (2015) Genetic transformation of Plumbago zeylanica with Agrobacterium rhizogenes strain LBA 9402 and characterization of transformed root lines. Plant Tissue Cult Biotech 25(1):21–35
Bathoju G, Rao K, Giri A (2017) Production of sapogenins (stigmasterol and hecogenin) from genetically transformed hairy root cultures of Chlorophytum borivilianum (Safed musli). Plant Cell Tiss Organ Cult 131(3):369–376
Belabbassi O, Khelifi-Slaoui M, Zaoui D, Benyammi R, Khalfallah N, Malik S, Makhzoum A, Khelifi L (2016) Synergistic effects of polyploidization and elicitation on biomass and hyoscyamine content in hairy roots of Datura stramonium. Biotechnol Agron Soc Environ 20(3):408–416
Benyammi R, Paris C, Khelifi-Slaoui M, Zaoui D, Belabbassi O, Bakiri N, Meriem Aci M, Harfi B, Malik S, Makhzoum A, Desobry S (2016) Screening and kinetic studies of catharanthine and ajmalicine accumulation and their correlation with growth biomass in Catharanthus roseus hairy roots. Pharm Biol 54(10):2033–2043
Carlín AP, Tafoya F, Solís AGA, Pérez-Molphe-Balch E (2015) Effects of different culture media and conditions on biomass production of hairy root cultures in six Mexican cactus species. In Vitro Cell Dev Biol Plant 51(3):332–339
Chashmi AN, Sharifi M, Behmanesh M (2016) Lignan enhancement in hairy root cultures of Linum album using coniferaldehyde and methylenedioxycinnamic acid. Prep Biochem Biotechnol 46(5):454–460
Cheruvathur MK, Thomas TD (2014) Effect of plant growth regulators and elicitors on rhinacanthin accumulation in hairy root cultures of Rhinacanthus nasutus (L.) Kurz. Plant Cell Tiss Organ Cult 118(1):169–177
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for another culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18(5):659–668
Chung IM, Rekha K, Rajakumar G, Thiruvengadam M (2016) Production of glucosinolates, phenolic compounds and associated gene expression profiles of hairy root cultures in turnip (Brassica rapa ssp. rapa). Biotech 6(2):175. https://doi.org/10.1007/s13205-016-0492-9
Coke JE (1996) Basal nutrient medium for in vitro cultures of loblolly pines. US Patent 5,534,434A, July 9, 1996
Condori J, Sivakumar G, Hubstenberger J, Dolan MC, Sobolev VS, Medina-Bolivar F (2010) Induced biosynthesis of resveratrol and the prenylated stilbenoids arachidin-1 and arachidin-3 in hairy root cultures of peanut: Effects of culture medium and growth stage. Plant Physiol Biochem 48(5):310–318
Danphitsanuparn P, Boonsnongcheep P, Boriboonkaset T, Chintapakorn Y, Prathanturarug S (2012) Effects of Agrobacterium rhizogenes strains and other parameters on production of isoflavonoids in hairy roots of Pueraria candollei Grah. ex Benth. var. candollei. Plant Cell Tiss Organ Cult 111(3):315–322
Dehghan E, Häkkinen ST, Oksman-Caldentey KM, Ahmadi FS (2012) Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.). Plant Cell Tiss Organ Cult 110(1):35–44
Driver JA, Kuniyuki AH (1984) In vitro propagation of Paradox walnut rootstock. Hort Sci 19:507–509
Gai QY, Jiao J, Luo M, Wang W, Ma W, Zu YG, Fu YJ (2015a) Establishment of high-productive Isatis tinctoria L. hairy root cultures: a promising approach for efficient production of bioactive alkaloids. Biochem Eng J 95:37–47
Gai QY, Jiao J, Luo M, Wei ZF, Zu YG, Ma W, Fu YJ (2015b) Establishment of hairy root cultures by Agrobacterium rhizogenes mediated transformation of Isatis tinctoria L. for the efficient production of flavonoids and evaluation of antioxidant activities. PloS One 10(3):p.e0119022
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50(1):151–158
Gelvin SB (2017) Integration of Agrobacterium T-DNA into the plant genome. Annu Rev Genet 51:195–217
Georgiev VG, Weber J, Kneschke EM, Denev PN, Bley T, Pavlov AI (2010) Antioxidant activity and phenolic content of betalain extracts from intact plants and hairy root cultures of the red beetroot Beta vulgaris cv. Detroit dark red. Plant Foods Hum Nutr 65(2):105–111
Goklany S, Rizvi NF, Loring RH, Cram EJ, Lee-Parsons CW (2013) Jasmonate-dependent alkaloid biosynthesis in Catharanthus roseus hairy root cultures is correlated with the relative expression of Orca and Zct transcription factors. Biotechnol Prog 29(6):1367–1376
Gupta PK, Durzan KJ (1985) Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana). Plant Cell Rep 4(4):177–179
Halder M, Jha S (2016) Enhanced trans-resveratrol production in genetically transformed root cultures of peanut (Arachis hypogaea L.). Plant Cell Tiss Organ Cult 124(3):555–572
Hanafy MS, Matter MA, Asker MS, Rady MR (2016) Production of indole alkaloids in hairy root cultures of Catharanthus roseus L. and their antimicrobial activity. S Afr J Bot 105:9–18
Harfi B, Khelifi-Slaoui M, Zaoui D, Benyammi R, Belabbassi O, Khelifi L (2011) Effect of culture medium on hyoscyamine production from four Datura sp hairy roots. Adv Environ Biol 5:1023–1031
Harfi B, Khelifi-Slaoui M, Bekhouche M, Benyammi R, Hefferon K, Makhzoum A, Khelifi L (2016) Hyoscyamine production in hairy roots of three Datura species exposed to high-salt medium. In Vitro Cell Dev Biol Plant 52(1):92–98
Hashemi SM, Naghavi MR (2016) Production and gene expression of morphinan alkaloids in hairy root culture of Papaver orientale L. using abiotic elicitors. Plant Cell Tiss Organ Cult 125(1):31–41
Heller R (1953) Recherches sur la nutrition minérale des tissus végétaux cultivés in vitro. Ann Sci Nat Bot Biol Veg 14:1–223
Hosseini SM, Bahramnejad B, Baneh HD, Emamifar A, Goodwin PH (2017) Hairy root culture optimization and resveratrol production from Vitis vinifera subsp. sylvesteris. World J Microbiol Biotechnol 33(4):67. https://doi.org/10.1007/s11274-017-2235-4
Huang B, Lin H, Yan C, Qiu H, Qiu L, Yu R (2014a) Optimal inductive and cultural conditions of Polygonum multiflorum transgenic hairy roots mediated with Agrobacterium rhizogenes R1601 and an analysis of their anthraquinone constituents. Pharmacogn Mag 10(37):77–82
Huang SH, Vishwakarma RK, Lee TT, Chan HS, Tsay HS (2014b) Establishment of hairy root lines and analysis of iridoids and secoiridoids in the medicinal plant Gentiana scabra. Bot Stud 55(1):17–25
Huang X, Yao J, Zhao Y, Xie D, Jiang X, Xu Z (2016) Efficient rutin and quercetin biosynthesis through flavonoids-related gene expression in Fagopyrum tataricum Gaertn. hairy root cultures with UV-B irradiation. Front Plant Sci 7:63. https://doi.org/10.3389/fpls.2016.00063
Jeong CS, Murthy HN, Hahn EJ, Lee HL, Paek KY (2009) Inoculum size and auxin concentration influence the growth of adventitious roots and accumulation of ginsenosides in suspension cultures of ginseng (Panax ginseng CA Meyer). Acta Physiol Plant 31:219–222
Jiao J, Gai QY, Fu YJ, Ma W, Peng X, Tan SN, Efferth T (2014) Efficient production of isoflavonoids by Astragalus membranaceus hairy root cultures and evaluation of antioxidant activities of extracts. J Agric Food Chem 62(52):12649–12658
Jiao J, Gai QY, Fu YJ, Ma W, Yao LP, Feng C, Xia XX (2015) Optimization of Astragalus membranaceus hairy roots induction and culture conditions for augmentation production of astragalosides. Plant Cell Tiss Organ Cult 120(3):1117–1130
Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126(2):105–110
Kim YS, Li X, Park WT, Uddin MR, Park NI, Kim YB, Lee MY, Park SU (2012) Influence of media and auxins on growth and falvone production in hairy root cultures of baikal skullcap, Scutellaria baicalensis. Plant Omics 5(1):24–27
Kim HH, Park JS, Park SU, Park NI (2017) Production of baicalin, baicalein and wogonin in hairy root culture of American skullcap (Scutellaria lateriflora) by auxin treatment. Biosci Biotech Res Asia 14(2):673–677
Kochan E, Szymańska G, Szymczyk P (2014) Effect of sugar concentration on ginsenoside biosynthesis in hairy root cultures of Panax quinquefolium cultivated in shake flasks and nutrient sprinkle bioreactor. Acta Physiol Plant 36(3):613–619
Kumar V, Desai D, Shriram V (2014) Hairy root induction in Helicteres isora L. and production of diosgenin in hairy roots. Nat Prod Bioprospect 4(2):107–112
Li B, Wang B, Li H, Peng L, Ru M, Liang Z, Yan X, Zhu Y (2016) Establishment of Salvia castanea Diels f. tomentosa Stib. hairy root cultures and the promotion of tanshinone accumulation and gene expression with Ag+, methyl jasmonate, and yeast extract elicitation. Protoplasma 253(1):87–100
Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue culture. Physiol Plant 18(1):100–127
Liu Q, Cui L, Guo Y, Ni X, Zhang Y, Kai G (2013) Optimization of nutritive factors in culture media for growth and tropane alkaloid production from Anisodus acutangulus hairy roots. J Appl Pharm Sci 3(01):001–004
Liu L, Yang D, Liang T, Zhang H, He Z, Liang Z (2016) Phosphate starvation promoted the accumulation of phenolic acids by inducing the key enzyme genes in Salvia miltiorrhiza hairy roots. Plant Cell Rep 35(9):1933–1942
Lloyd G, McCown B (1981) Commercially feasible micropropagation of Mountain Laurel, Kalmia latifolia, by use of shoot tip culture. Comb Proc Int Plant Propagator Soc 30:421–427
Mehrotra S, Prakash O, Khan F, Kukreja AK (2013) Efficiency of neural network-based combinatorial model predicting optimal culture conditions for maximum biomass yields in hairy root cultures. Plant cell Rep 32(2):309–317
Mukherjee C, Samanta T, Mitra A (2016) Redirection of metabolite biosynthesis from hydroxybenzoates to volatile terpenoids in green hairy roots of Daucus carota. Planta 243:305–320
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479
Murthy HN, Lee EJ, Paek KY (2014) Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell Tiss Organ Cult 118:1–16
Nagella P, Thiruvengadam M, Jung SJ, Murthy HN, Chung IM (2013) Establishment of Gymnema sylvestre hairy root cultures for the production of gymnemic acid. Acta Physiol Plant 35(10):3067–3073
Nandagopal K, Halder M, Dash B, Nayak S, Jha S (2017) Biotechnological approaches for production of anti-cancerous compounds resveratrol, podophyllotoxin and zerumbone. Curr Med Chem. https://doi.org/10.2174/0929867324666170404145656
Nayak P, Sharma M, Behera SN, Thirunavoukkarasu M, Chand PK (2015) High-performance liquid chromatographic quantification of plumbagin from transformed rhizoclones of Plumbago zeylanica L.: Inter-clonal variation in biomass growth and plumbagin production. Appl Biochem Biotechnol 175:1745–1770
Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163(3862):85–87
Nourozi E, Hosseini B, Hassani A (2014) A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum. Biologia 69(7):870–879
Pandey H, Pandey P, Pandey SS, Singh S, Banerjee S (2016) Meeting the challenge of stevioside production in the hairy roots of Stevia rebaudiana by probing the underlying process. Plant Cell Tiss Organ Cult 126(3):511–521
Parr AJ (2017) Secondary products from plant cell cultures–early experiences with Agrobacterium rhizogenes-transformed hairy roots. In: Jha S (ed) Transgenesis and secondary metabolism. Reference series in phytochemistry. Springer International Publishing, Cham, pp 253–266
Patra N, Srivastava AK (2014a) Enhanced production of artemisinin by hairy root cultivation of Artemisia annua in a modified stirred tank reactor. Appl Biochem Biotechnol 174(6):2209–2222
Patra N, Srivastava AK (2014b) Mass scale artemisinin production in a stirred tank bioreactor using hairy roots of Artemisia annua. Int J Biosci Biochem Bioinforma 4(6):467–474
Patra N, Srivastava AK (2015) Use of model-based nutrient feeding for improved production of artemisinin by hairy roots of Artemisia annua in a modified stirred tank bioreactor. Appl Biochem Biotechnol 177(2):373–388
Patra N, Srivastava AK (2017) Mass production of artemisinin using hairy root cultivation of Artemisia annua in bioreactor. In: Pavlov A, Bley T (eds) Bioprocessing of plant in vitro systems. Springer International Publishing, Cham, pp 1–17
Perassolo M, Cardillo AB, Mugas ML, Montoya SCN, Giulietti AM, Talou JR (2017) Enhancement of anthraquinone production and release by combination of culture medium selection and methyl jasmonate elicitation in hairy root cultures of Rubia tinctorum. Ind Crops Prod 105:124–132
Peret B, Clement M, Nussaume L, Desnos T (2011) Root developmental adaptation to phosphate starvation: better safe than sorry. Trends Plant Sci 16:1360–1385
Petrova M, Zayova E, Vlahova M (2013) Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes. Open Life Sci 8(5):470–479
Piątczak E, Kuźma Ł, Wysokińska H (2016) The influence of methyl jasmonate and salicylic acid on secondary metabolite production in Rehmannia glutinosa Libosch. hairy root culture. Acta Biologica Cracoviensia s. Botanica 58(1):57–65
Pillai DB, Jose B, Satheeshkumar K, Krishnan PN (2015) Optimization of inoculum density in hairy root culture of Plumbago rosea L. for enhanced growth and plumbagin production towards scaling-up in bioreactor. Indian J Biotechnol 14:264–269
Prakash O, Mehrotra S, Krishna A, Mishra BN (2010) A neural network approach for the prediction of in vitro culture parameters for maximum biomass yields in hairy root cultures. J Theor Biol 265(4):579–585
Praveen N, Murthy HN (2012) Synthesis of withanolide A depends on carbon source and medium pH in hairy root cultures of Withania somnifera. Ind Crops Prod 35(1):241–243
Praveen N, Murthy HN (2013) Withanolide A production from Withania somnifera hairy root cultures with improved growth by altering the concentrations of macro elements and nitrogen source in the medium. Acta Physiol Plant 35(3):811–816
Rahimi S, Hasanloo T (2016) The effect of temperature and pH on biomass and bioactive compounds production in Silybum marianum hairy root cultures. Res J Pharmacogn 3(2):53–59
Ray S, Majumder A, Bandyopadhyay M, Jha S (2014) Genetic transformation of sarpagandha (Rauvolfia serpentina) with Agrobacterium rhizogenes for identification of high alkaloid yielding lines. Acta Physiol Plant 36(6):1599–1605
Roychowdhury D, Majumder A, Jha S (2013) Agrobacterium rhizogenes-mediated transformation in medicinal plants: prospects and challenges. In: Chandra S, Lata H, Varma A (eds) Biotechnology for medicinal plants. Springer, Berlin Heidelberg, pp 29–68
Roychowdhury D, Halder M, Jha S (2017) Agrobacterium rhizogenes-mediated transformation in medicinal plants: genetic stability in long-term culture. In: Jha S (ed) Transgenesis and secondary metabolism. Springer, Cham, pp 323–345
Sangwan RS, Chaurasiya ND, Lal P, Misra L, Tuli R, Sangwan NS (2008) Withanolide A is inherently de novo biosynthesized in roots of the medicinal plant Ashwagandha (Withania somnifera). Physiol Plant 133(2):278–287
Saravanakumar A, Aslam A, Shajahan A (2012) Development and optimization of hairy root culture systems in Withania somnifera (L.) Dunal for withaferin-A production. Afr J Biotechnol 11(98):16412–16420
Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50(1):199–204
Sharafi A, Sohi HH, Mousavi A, Azadi P, Razavi K, Ntui VO (2013) A reliable and efficient protocol for inducing hairy roots in Papaver bracteatum. Plant Cell Tiss Organ Cult 113(1):1–9
Shi M, Luo X, Ju G, Li L, Huang S, Zhang T, Wang H, Kai G (2016) Enhanced diterpene tanshinone accumulation and bioactivity of transgenic Salvia miltiorrhiza hairy roots by pathway engineering. J Agric Food Chem 64(12):2523–2530
Shinde AN, Malpathak N, Fulzele DP (2010) Impact of nutrient components on production of the phytoestrogens daidzein and genistein by hairy roots of Psoralea corylifolia. J Nat Med 64(3):346–353
Sivanandhan G, Rajesh M, Arun M, Jeyaraj M, Dev GK, Manickavasagam M, Selvaraj N, Ganapathi A (2012) Optimization of carbon source for hairy root growth and withaferin A and withanone production in Withania somnifera. Nat Prod Commun 7(10):1271–1272
Srivastava S, Srivastava AK (2014) Effect of elicitors and precursors on azadirachtin production in hairy root culture of Azadirachta indica. Appl Biochem Biotechnol 172(4):2286–2297
Srivastava V, Mehrotra S, Mishra S (2017a) Biotransformation through hairy roots: perspectives, outcomes, and major challenges. In: Jha S (ed) Transgenesis and secondary metabolism. Reference series in phytochemistry. Springer, Cham, pp 347–370
Srivastava V, Mehrotra S, Verma PK (2017b) Biotechnological interventions for production of therapeutic secondary metabolites using hairy root cultures of medicinal plants. In: Dubey SK, Pandey A, Sangwan RS (eds) Current developments in biotechnology and bioengineering: Crop modification, nutrition, and food production, vol 17. Elsevier, Netherland, pp 259–282
Stiles AR, Liu CZ (2013) Hairy root culture: bioreactor design and process intensification. In: Doran PM (ed) Biotechnology of hairy root systems. Springer, Berlin Heidelberg, pp 91–114
Sujatha G, Zdravković-Korać S, Ćalić D, Flamini G, Kumari BR (2013) High-efficiency Agrobacterium rhizogenes-mediated genetic transformation in Artemisia vulgaris: hairy root production and essential oil analysis. Ind Crops Prod 44:643–652
Sun J, Peebles CA (2016) Engineering overexpression of ORCA3 and strictosidine glucosidase in Catharanthus roseus hairy roots increases alkaloid production. Protoplasma 253(5):1255–1264
Tepfer D (2017) DNA Transfer to Plants by Agrobacterium rhizogenes: A Model for Genetic Communication Between Species and Biospheres. In: Jha S (ed) Transgenesis and secondary metabolism, reference series in phytochemistry. Springer International Publishing, Cham, pp 3–43
Thakore D, Srivastava AK, Sinha AK (2017) Mass production of ajmalicine by bioreactor cultivation of hairy roots of Catharanthus roseus. Biochem Eng J 119:84–91
Thiruvengadam M, Praveen N, Kim EH, Kim SH, Chung IM (2014) Production of anthraquinones, phenolic compounds and biological activities from hairy root cultures of Polygonum multiflorum Thunb. Protoplasma 251(3):555–566
Thiruvengadam M, Rekha K, Chung IM (2016) Induction of hairy roots by Agrobacterium rhizogenes-mediated transformation of spine gourd (Momordica dioica Roxb. ex. willd) for the assessment of phenolic compounds and biological activities. Sci Hort 198:132–141
Vaghari H, Jafarizadeh-Malmiri H, Anarjan N, Berenjian A (2017) Hairy root culture: A biotechnological approach to produce valuable metabolites. In: Meena VS, Mishra PK, Bisht JK, Pattanayak A (eds) Agriculturally important microbes for sustainable agriculture. Springer, Singapore, pp 131–160
Verma PC, Singh H, Negi AS, Saxena G, Rahman LU, Banerjee S (2015) Yield enhancement strategies for the production of picroliv from hairy root culture of Picrorhiza kurroa Royle ex Benth. Plant Signal Behav 10(5):e1023976. https://doi.org/10.1080/15592324.2015.1023976
Vinterhalter B, Krstić-Milošević D, Janković T, Pljevljakušić D, Ninković S, Smigocki A, Vinterhalter D (2015) Gentiana dinarica Beck. hairy root cultures and evaluation of factors affecting growth and xanthone production. Plant Cell Tiss Organ Cult 121(3):667–679
Wang JW, Wu JY (2013) Effective elicitors and process strategies for enhancement of secondary metabolite production in hairy root cultures. In: Doran PM (ed) Biotechnology of hairy root systems. Springer, Berlin Heidelberg, pp 55–89
Wawrosch C, Schwaiger S, Stuppner H, Kopp B (2014) Lignan formation in hairy root cultures of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter). Fitoterapia 97:219–223
Weremczuk-Jeżyna I, Skała E, Olszewska MA, Kiss AK, Balcerczak E, Wysokińska H, Kicel A (2016) The identification and quantitative determination of rosmarinic acid and salvianolic acid B in hairy root cultures of Dracocephalum forrestii WW Smith. Ind Crops Prod 91:125–131
White PR (1963) The cultivation of animal and plant cells, 2nd edn. Ronald Press, New York
Yu KW, Murthy HN, Hahn EJ, Paek KY (2005) Ginsenoside production by hairy root cultures of Panax ginseng: influence of temperature and light quality. Biochem Eng J 23(1):53–56
Zhao L, Sander GW, Shanks JV (2013) Perspectives of the metabolic engineering of terpenoid indole alkaloids in Catharanthus roseus hairy roots. In: Doran PM (ed) Biotechnology of hairy root systems. Springer, Berlin Heidelberg, pp 23–54
Zhu C, Miao G, Guo J, Huo Y, Zhang X, Xie J, Feng J (2014) Establishment of Tripterygium wilfordii Hook. f. hairy root culture and optimization of its culture conditions for the production of triptolide and wilforine. J Microbiol Biotechnol 24:823–834
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SJ is thankful to the National Academy of Sciences (NASI, Allahabad, India), for the award of Platinum Jubilee Senior Scientist Fellowship and providing the financial support to continue the research.
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Halder, M., Roychowdhury, D., Jha, S. (2018). A Critical Review on Biotechnological Interventions for Production and Yield Enhancement of Secondary Metabolites in Hairy Root Cultures. In: Srivastava, V., Mehrotra, S., Mishra, S. (eds) Hairy Roots. Springer, Singapore. https://doi.org/10.1007/978-981-13-2562-5_2
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