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Conventional and Biotechnological Approaches to Enhance Steviol Glycosides (SGs) in Stevia rebaudiana Bertoni

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Biotechnological Approaches for Medicinal and Aromatic Plants

Abstract

Stevia rebaudiana Bertoni (Asteraceae) is a perennial herb with many secondary metabolites present mainly in the leaf and other plant parts. Major secondary metabolites, for which the plant is consumed, are steviol glycosides (SGs) containing diterpene steviol, attached to which are one to four molecules of glucose by glycosidic bond(s). They impart very less calorie in consumer’s diet, thus widely used as a sweetener in food and beverage industries. The amount of SGs in the plant varies from 8 to 10%, enhancement of which is always in demand. Both conventional and biotechnological approaches are being made till date to increase the level of SGs in the plant. In the present chapter, we discussed various ways to enhance the level of these sweeteners with the prime focus on conventional and biotechnological approaches.

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References

  • Aman, N., Hadi, F., Khalil, S. A., et al. (2013). Efficient regeneration for enhanced steviol glycosides production in Stevia rebaudiana (Bertoni). Comptes Rendus Biologies, 336, 486–492.

    Article  CAS  PubMed  Google Scholar 

  • Bayraktar, M., Naziri, E., Akgun, I. H., et al. (2016). Elicitor induced stevioside production, in vitro shoot growth, and biomass accumulation in micropropagated Stevia rebaudiana. Plant Cell, Tissue and Organ Culture, 127, 289–300.

    Article  CAS  Google Scholar 

  • Bondarev, N., Reshetnyak, O., & Nosov, A. (2002). Features of development of Stevia rebaudiana shoots cultivated in the roller bottle bioreactor and their production of steviol glycosides. Planta Medica, 68, 759–762.

    Article  CAS  PubMed  Google Scholar 

  • Brandle, J. E., & Telmer, P. G. (2007). Steviol glycosides biosynthesis. Phytochemistry, 68, 1855–1863.

    Article  CAS  PubMed  Google Scholar 

  • Brandle, J. E., Richman, A., Swanson, A. K., et al. (2002). Leaf ESTs from Stevia rebaudiana: A resource for gene discovery in diterpene synthesis. Plant Molecular Biology, 50(4), 612–622.

    Google Scholar 

  • Ceunen, S., Werbrouck, S., & Geuns, J. M. C. (2012). Stimulation of steviol glycoside accumulation in Stevia rebaudiana by LED light. Journal of Plant Physiology, 169, 749–752.

    Article  CAS  PubMed  Google Scholar 

  • Chen, J., Hou, K., Qin, P., et al. (2014). RNA-Seq for gene identification and transcript profiling of three Stevia rebaudiana genotypes. BMC Genomics, 15, 571–581.

    Article  PubMed  PubMed Central  Google Scholar 

  • Copetta, A., Lingua, G., & Berta, G. (2006). Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil production in Ocimum basilicum L. var. Genovese. Mycorrhiza, 16, 485–499.

    Article  CAS  PubMed  Google Scholar 

  • Evans, J. M. (2014). Genetic and environment control of steviol glycoside biosynthesis in Stevia rebaudiana Bertoni. Thesis, Michigan State University.

    Google Scholar 

  • Fu, X., Yin, Z. P., Chen, J. G., et al. (2015). Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana. Journal of Agricultural and Food Chemistry, 63(1), 262–268.

    Article  CAS  PubMed  Google Scholar 

  • Guleria, P., & Yadav, S. K. (2013). Agrobacterium mediated transient gene silencing (AMTS) in Stevia rebaudiana: Insights into steviol glycoside biosynthesis pathway. PLoS One, 8, e74731. https://doi.org/10.1371/journal.pone.0074731.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Gupta, P., Sharma, S., & Saxena, S. (2010). Callusing in Stevia rebaudiana (Natural Sweetener) for steviol glycoside production. World Academy of Science, Engineering and Technology, 48, 572–576.

    Google Scholar 

  • Gupta, N., Gudipati, T., Bhaduria, R., et al. (2016). Influence of light, growth regulators, nitrate and sugars on the production of stevioside and rebaudioside a on the leaf callus culture of Stevia rebaudiana Bertoni. International Journal of Applied Biology and Pharmaceutical Technology, 7(2), 205–213.

    CAS  Google Scholar 

  • Hajihashemi, S., & Geuns, J. M. C. (2016). Gene transcription and steviol glycoside accumulation in Stevia rebaudiana under polyethylene glycol-induced drought stress in greenhouse cultivation. FEBS Open Bio, 6, 937–944.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ilaei, Z. A., Maleki, M., & Omidi, M. (2016). Production of stevioside by hairy root cultures of Stevia rebaudiana Bertoni transformed by Agrobacterium rhizogenes. In Abstract of the 2nd international and 14th Iranian genetics congress, Sh. Beheshti University, Tehran, 21–23 May 2016.

    Google Scholar 

  • Karuppusamy, S. (2009). A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. Journal of Medicinal Plant Research, 3(13), 1222–1239.

    CAS  Google Scholar 

  • Kaur, R., Sharma, N., & Raina, R. (2015). Identification and functional annotation of expressed sequence tags based SSR markers of Stevia rebaudiana. Turkish Journal of Agriculture and Forestry, 39, 439–450.

    Article  CAS  Google Scholar 

  • Khalil, S. A., Zamir, R., & Ahmad, N. (2014). Effect of different propagation techniques and gamma irradiation on major steviol glycoside’s content in Stevia rebaudiana. The Journal of Animal and Plant Sciences, 24(6), 1743–1751.

    Google Scholar 

  • Kumar, H., Kaul, K., Gupta, S. B., et al. (2012). A comprehensive analysis of fifteen genes of steviol glycosides biosynthesis pathway in Stevia rebaudiana (Bertoni). Gene, 492, 276–284.

    Article  CAS  PubMed  Google Scholar 

  • Mandal, S., Eveling, H., Giri, B., et al. (2013). Arbuscular mycorrhiza enhances the production of stevioside and rebaudioside A in Stevia rebaudiana via nutritional and non-nutritional mechanisms. Applied Soil Ecology, 72, 187–194.

    Article  Google Scholar 

  • Mandal, S., Upadhyay, S., Singh, V. P., et al. (2015). Enhanced production of steviol glycosides in mycorrhizal plants: A concerted effect of arbuscular mycorrhizal symbiosis on transcription of biosynthetic genes. Plant Physiology and Biochemistry, 89, 100–106.

    Article  CAS  PubMed  Google Scholar 

  • Mandhan, V., Kaur, J., & Singh, K. (2012). smRNAome profiling to identify conserved and novel microRNAs in Stevia rebaudiana Bertoni. BMC Plant Biology, 12, 197–211.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Megeji, N. W., Kumar, J. K., Singh, V., et al. (2005). Introducing Stevia rebaudiana, a natural zero-calorie sweetener. Current Science, 88(5), 801–804.

    CAS  Google Scholar 

  • Michalec-Warczecha, Z., Pistelli, L., D’angiolillo, F., et al. (2016). Establishment of highly efficient Agrobacterium rhizogenes-mediated transformation for Stevia rebaudiana Bertoni explants. Acta Biologica Cracoviensia Series Botanica, 58(1), 113–118.

    Article  CAS  Google Scholar 

  • Modi, A. R. (2013). Differential expression of genes involved in steviol glycoside biosynthesis in Stevia rebaudiana Bertoni, thesis. Anand Agricultural University.

    Google Scholar 

  • Modi, A. R., Shukla, Y. M., Litoriya, N. S., et al. (2011). Effect of gibberellic acid foliar spray on growth parameters and stevioside content of ex vitro grown plants of Stevia rebaudiana Bertoni. Medicinal Plants, 3(2), 157–160.

    Google Scholar 

  • Modi, A., Litoriya, N., Prajapati, V., et al. (2014a). Transcriptional profiling of genes involved in steviol glycoside biosynthesis in Stevia rebaudiana Bertoni during plant hardening. Developmental Dynamics, 243, 1067–1073.

    Article  CAS  PubMed  Google Scholar 

  • Modi, A. R., Raj, S., Kanani, P., et al. (2014b). Analysis of differentially expressed genes involved in stevioside biosynthesis in cultures of Stevia rebaudiana Bertoni treated with steviol as an immediate precursor. Journal of Plant Growth Regulation, 33, 481–488.

    Article  CAS  Google Scholar 

  • Pal, P. K., Kumar, R., Guleria, V., et al. (2015). Crop-ecology and nutritional variability influence growth and secondary metabolites of Stevia rebaudiana Bertoni. BMC Plant Biology, 15, 67–82.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pandey, M., & Chikara, S. K. (2015). Effect of salinity and drought stress on growth parameters, glycoside content and expression levels of vital genes in steviol glyocosides biosynthesis pathway of Stevia rebaudiana (Bertoni). International Journal of Genetics, 7(1), 153–160.

    CAS  Google Scholar 

  • Pickens, L. B., Tang, T., & Choi, Y. H. (2011). Metabolic engineering for the production of natural products. Annual Review of Chemistry Biomolecular Engineering, 2, 211–236.

    Article  CAS  Google Scholar 

  • Richman, S. A., Gijzen, M., Starratt, A. N., et al. (1999). Diterpene synthesis in Stevia rebaudiana: Recruitment and up-regulation of key enzymes from the gibberellin biosynthetic pathway. The Plant Journal, 19(4), 411–421.

    Article  CAS  PubMed  Google Scholar 

  • Sreedhar, R. V., Venkatachalam, L., Thimmaraju, R., et al. (2008). Direct organogenesis from leaf explants of Stevia rebaudiana and cultivation in bioreactor. Biologia Plantarum, 52(2), 355–360.

    Article  CAS  Google Scholar 

  • Striedner, J., Geissler, S., Czygan, F. C., et al. (2004). Contributions to the biotechnological production of sweeteners from Stevia rebaudiana bertoni. III. Accumulation of secondary metabolites by means of a precursor and by elicitation of cell cultures. Acta Biotechnologica, 11(5), 505–509.

    Article  Google Scholar 

  • Tadhani, M. B., Patel, V. H., & Subhash, R. (2007). In vitro antioxidant activities of Stevia rebaudiana leaves and callus. Journal of Food Composition and Analysis, 27, 323–329.

    Article  CAS  Google Scholar 

  • Toussaint, J. P., Smith, F. A., & Smith, S. E. (2007). Arbuscular mycorrhizal fungi can induce the production of phytochemicals in sweet basil irrespective of phosphorous nutrition. Mycorrhiza, 17, 291–297.

    Article  CAS  Google Scholar 

  • Veau, B., Courtois, M., Oudin, A., et al. (2000). Cloning and expression of cDNAs encoding two enzymes of the MEP pathway in Catharanthus roseus. Biochimica et Biophysica Acta, 1517, 159–163.

    Article  CAS  PubMed  Google Scholar 

  • Walter, M. A., Hans, J., & Strack, D. (2000). Two distantly related genes encoding 1-deoxy-D-xylulose-5-phosphate synthases: Differential regulation in shoots and apocarotenoid-accumulating mycorrhizal roots. The Plant Journal, 31(3), 243–253.

    Article  Google Scholar 

  • Yadav, A. K., Singh, S., Dhyani, D., et al. (2010). A review on the improvement of stevia [Stevia rebaudiana (Bertoni) ]. Canadian Journal of Plant Science, 91, 1–27.

    Article  Google Scholar 

  • Yamazaki, T., & Flores, H. E. (1991). Examination of steviol glycosides production by hairy root and shoot cultures of Stevia rebaudiana. Journal of Natural Products, 54(4), 986–992.

    Article  CAS  Google Scholar 

  • Yu, X., Yang, J., Wang, E., et al. (2015). Effects of growth stages and fulvic acid on the diversity and dynamics of endophytic bacterial community in Stevia rebaudiana Bertoni leaves. Frontiers in Microbiology, 6, 867–879.

    PubMed  PubMed Central  Google Scholar 

  • Yucesan, B., Buyukgocmen, R., Mohammed, A., et al. (2016). An efficient regeneration system and steviol glycoside analysis of Stevia rebaudiana Bertoni, a source of natural high-intensity sweetener. In Vitro Cellular & Developmental Biology Plant, 52, 330–337.

    Article  CAS  Google Scholar 

  • Zubek, S., Mielcarek, S., & Turnau, K. (2012). Hypericin and pseudohypericin concentrations of a valuable medicinal plant Hepericum perforatum L. are enhanced by arbuscular mycorrhizal fungi. Mycorrhiza, 22, 149–156.

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Institute of Plant Sciences, Agricultural Research Organization, Rishon LeZion, Israel

    Arpan Modi

  2. Department of Biotechnology, School of Earth, Biological and Environmental Sciences, Central University of South Bihar, Gaya, Bihar, India

    Nitish Kumar

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  1. Arpan Modi
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  2. Nitish Kumar
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Editors and Affiliations

  1. Department of Biotechnology, Central University of South Bihar, Panchanpur, Gaya, Bihar, India

    Nitish Kumar

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Modi, A., Kumar, N. (2018). Conventional and Biotechnological Approaches to Enhance Steviol Glycosides (SGs) in Stevia rebaudiana Bertoni. In: Kumar, N. (eds) Biotechnological Approaches for Medicinal and Aromatic Plants. Springer, Singapore. https://doi.org/10.1007/978-981-13-0535-1_3

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