Skip to main content
SpringerLink
Log in

Genetic Improvement of Medicinal and Aromatic Plants Through Haploid and Double Haploid Development

  • Chapter
  • First Online:
Biotechnological Approaches for Medicinal and Aromatic Plants

Abstract

Medicinal and aromatic plants (MAPs) produce secondary metabolites that are pharmacologically and economically important. These compounds are distributed/limited in a particular species, genus, or family and are reported to play an important ecological role like pollinator attractants, adaptations to environmental and biological stresses (chemical defenses). The concentration of these secondary metabolites is very low and highly variable, thus making them high-value low-volume products. Advances in biotechnology, particularly haploid and double haploid (DH) production, have opened new avenues for breeding, genetics, transformation, and mapping studies in these MAPs. This will allow means for the commercial exploitation of such rare plants and the chemicals they produce in medicines, aromatic industries, and plant growth and for insect and weed control. This chapter details the different methods of producing haploids and DHs, factors influencing their generation, and their use in genetic improvement of these MAPs. Few MAP species in which haploid and DHs have been studied are also briefly discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Abbasi, B. H., Saxena, P. K., Murch, S. J., & Liu, C. Z. (2007). Echinacea biotechnology: Challenges and opportunities. In Vitro Cellular & Developmental Biology. Plant, 43, 481–492.

    Article  CAS  Google Scholar 

  • Abdollahi, M. R., Moieni, A., & Javaran, M. J. (2004). Interactive effects of shock and culture density on embryo induction in isolated microspore culture of Brassica napus L. cv. Global Iranian Journal of Biotechnology, 2, 97–100.

    Google Scholar 

  • Abdollahi, M. R., et al. (2009). An efficient method for transformation of pre-androgenic, isolated Brassica napus microspores involving microprojectile bombardment and Agrobacterium-mediated transformation. Acta Physiologiae Plantarum, 31(6), 1313–1317.

    Google Scholar 

  • Adamus, A., & Michalik, B. (2003). Anther cultures of carrot (Daucus carota L.). Folia Hortic, 15, 49–58.

    Google Scholar 

  • Åhman, I. M., Kazachkova, N. I., Kamnert, I. M., Hagberg, P. A., Dayteg, C. I., Eklund, G. M., Meijer, L. J. O., & Ekbom, B. (2006). Characterisation of transgenic oilseed rape expressing pea lectin in anthers for improved resistance to pollen beetle. Euphytica, 151(3), 321–330.

    Google Scholar 

  • Ahmadi, B., Ghadimzadeh, M., Moghaddam, A. F., & Alizadeh, K. (2011). Embryogenesis and plant regeneration from isolated microspores of Brassica napus L. under different incubation time. Journal of Food, Agriculture & Environment, 9(3&4), 434–437.

    Google Scholar 

  • Allshire, R. C., & Karpen, G. H. (2008). Epigenetic regulation of centromeric chromatin: Old dogs, new tricks? Nature Reviews. Genetics, 9(12), 923–937.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Amatriaín, M. M., Svensson, J. T., Castillo, A. M., Cistué, L., Close, T. J., & Vallés, M. P. (2006). Transcriptome analysis of barley anthers: Effect of mannitol treatment on microspore embryogenesis. Physiologia Plantarum, 127, 551–560.

    Article  CAS  Google Scholar 

  • Amatriaín, M. M., Svensson, J. T., Castillo, A. M., Close, T. J., & Vallés, M. P. (2009). Microspore embryogenesis: Assignment of genes to embryo formation and green vs. albino plant production. Functional & Integrative Genomics, 9, 311–323.

    Article  CAS  Google Scholar 

  • Andersen, S. B. (2005). Haploids in the improvement of woody species. In C. E. Palmer, W. A. Keller, & K. Kasha (Eds.), Haploids in crop improvement II (Vol. 56, pp. 243–257). Heidelberg: Springer.

    Chapter  Google Scholar 

  • Andersen, B., Christiansen, I., & Arestveit, B. F. (1990). Carrot (Daucus carota L.): In vitro production of haploids and field trials 1 biotechnology in agriculture and forestry. In Y. P. S. Bajaj (Ed.), Haploids in crop improvement (Vol. 12). Berlin: Springer.

    Google Scholar 

  • Ashok Kumar, H. G., & Murthy, H. N. (2004). Effect of sugars and amino acids on Androgenesis of Cucumis sativus. Plant Cell Tissue and Organ Culture, 78(3), 201–208.

    Article  Google Scholar 

  • Aulinger, I. E., Peter, S. O., Schmid, J. E., & Stamp, P. (2003). Rapid attainment of a doubled haploid line from transgenic maize (Zea mays L.) plants by means of anther culture. In Vitro Cellular & Developmental Biology. Plant, 39(2), 165–170.

    Article  Google Scholar 

  • Bajaj, Y. P. S. (1990). In vitro production of haploids and their use in cell genetics and plant breeding. In Biotechnology in agriculture and forestry (Vol. 12, pp. 3–44). Berlin: Springer.

    Google Scholar 

  • Bandoniene, D., & Murkovic, M. (2002). The detection of radical scavenging compounds in crude extract of borage (Borago officinalis L.) by using an on-line HPLC-DPPH method. Journal of Biochemical and Biophysical Methods, 53(1–3), 45–49.

    Article  PubMed  CAS  Google Scholar 

  • Barrett, B. (2003). Medicinal properties of Echinacea: A critical review. Phytomedicine, 10, 66–86.

    Article  PubMed  CAS  Google Scholar 

  • Bauer, R., & Wagner, H. (1991). Echinacea species as potential immune stimulatory drugs. In H. Wagner & N. R. Farnsworth (Eds.), Economic and medicinal plant research (pp. 253–321). New York: Academic.

    Google Scholar 

  • Bhat, J. G., & Murthy, H. N. (2007). Factors affecting in-vitro gynogenic haploid production in niger (Guizotia abyssinica (L. f.) Cass.). Plant Growth Regulation, 52, 241–248.

    Article  CAS  Google Scholar 

  • Bhatti, S., Aziz, A. N., & Sauve, R. (2001). Anther culture response of Echinacea cultivars. In 23rd annual university-wide research symposium, Tennessee State University, Nashville, TN, 20–21 March 2001.

    Google Scholar 

  • Biessels, G. J., Smale, S., Duis, S. E. J., Kamal, A., & Gispen, W. H. (2001). The effect of gamma-linolenic acid-alpha-lipoic acid on functional deficits in the peripheral and central nervous system of streptozotocin-diabetic rats. Journal of the Neurological Sciences, 182(2), 99–106.

    Article  PubMed  CAS  Google Scholar 

  • Bilichak, A., Luu, J., & Eudes, F. (2015). Intracellular delivery of fluorescent protein into viable wheat microspores using cationic peptides. Frontiers in Plant Science, 28.

    Google Scholar 

  • Binarova, P., Hause, G., Cenklová, V., Cordewener, J. H. G., & Campagne, M. M. L. (1997). A short severe heat shock is required to induce embryogenesis in late bicellular pollen of Brassica napus L. Sexual Plant Reproduction, 10(4), 200–208.

    Article  Google Scholar 

  • Bishayee, A., Sarkar, A., & Chatterjee, M. (1995). Hepatoprotective activity of carrot (Daucus carota L.) against carbon tetrachloride intoxication in mouse liver. Journal of Ethnopharmacology, 47(2), 69–74.

    Article  PubMed  CAS  Google Scholar 

  • Biswas, K., Kumar, A., Babaria, B. A., Prabhu, K., & Setty, R. S. (2010). Hepatoprotective effect of leaves of Peltophorum pterocarpum against paracetamol induced acute liver damage in rats. Journal of Basic and Clinical Pharmacology, 1(1), 10–15.

    Google Scholar 

  • Blakeslee, A. F., Belling, J., Farnham, M. E., & Bergner, A. D. (1922). A haploid mutant in the Jimson weed, Datura stramonium. Science, 55, 646–647.

    Article  PubMed  CAS  Google Scholar 

  • Bohanec, B. (2003). Ploidy determination using flow cytometry. In Doubled haploid production in crop plants: A manual, Maluszynski

    Google Scholar 

  • Bohanec, B. (2009). Doubled haploid via gynogenesis. In A. Touraev, B. P. Forster, & S. M. Jain (Eds.), Advances in haploid production in higher plants (pp. 35–46). Berlin: Springer.

    Chapter  Google Scholar 

  • Bohanec, B., & Jakše, M. (1999). Variations in gynogenic response among long-day onion (Allium cepa L.) accessions. Plant Cell Reports, 18(9), 737–742.

    Article  CAS  Google Scholar 

  • Bohanec, B., Neskovic, M., & Vujicic, R. (1993). Anther culture and androgenetic plant regeneration in buckwheat (Fagopyrum esculentum Moench). Plant Cell, Tissue and Organ Culture, 35, 259–266.

    Article  Google Scholar 

  • Bohanec, B., Jakše, M., & Przywara, L. (2005). The development of onion (Allium cepa L.) embryo sacs in vitro and gynogenesis induction in relation to flower size. In Vitro Cellular & Developmental Biology. Plant, 41(4), 446–452.

    Article  Google Scholar 

  • Bueno, M. A., Gómez, A., Boscaiu, M., Manzanera, J. A., & Vicente, O. (1997). Stress-induced formation of haploid plants through anther culture in cork oak (Quercus suber). Physiologia Plantarum, 99(2), 335–341.

    Article  CAS  Google Scholar 

  • Cabral, A. L., Jordan, M. C., McCartney, C. A., You, F. M., Gavin Humphreys, D., MacLachlan, R., & Pozniak, C. J. (2014). Identification of candidate genes, regions and markers for pre-harvest sprouting resistance in wheat (Triticum aestivum L.). BMC Plant Biology, 14(1), 1–12

    Google Scholar 

  • Calleberg, E., & Johansson, L. (1996). Effect of gelling agents on anther cultures. In S. M. Jain, S. K. Sopory, & R. E. Veilleux (Eds.), In vitro haploid production in higher plants (Vol. 23, pp. 189–203). Dordrecht: Springer.

    Chapter  Google Scholar 

  • Caredda, S., Doncoeur, C., Devaux, P., Sangwan, R. S., & Clément, C. (2000). Plastid differentiation during androgenesis in albino and nonalbino producing cultivars of barley (Hordeum vulgare L.). Sexual Plant Reproduction, 13, 95–104.

    Article  CAS  Google Scholar 

  • Caredda, S., Devaux, P., Sangwan, R. S., Proult, I., & Clément, C. (2004). Plastid ultrastructure and DNA related to albinism in androgenetic embryos of various barley (Hordeum vulgare) cultivars. Plant Cell, Tissue and Organ Culture, 76, 35–43.

    Article  CAS  Google Scholar 

  • Chang, M., Huang, Y. W., Aronstam, R. S., & Lee, H. J. (2014). Cellular delivery of noncovalently-associated macromolecules by cell-penetrating peptides. Current Pharmaceutical Biotechnology, 15, 267–275.

    Google Scholar 

  • Chaturvedi, R., Razdan, M. K., & Bhojwani, S. S. (2003). Production of haploids of neem (Azadirachta indica A. Juss.) by anther culture. Plant Cell Reports, 21, 531–537.

    PubMed  CAS  Google Scholar 

  • Chauhan, H., & Khurana, P. (2011). Use of doubled haploid technology for development of stable drought tolerant bread wheat (Triticum aestivum L.) transgenics. Plant Biotechnology Journal, 9, 408.

    Article  PubMed  CAS  Google Scholar 

  • Chauvin, J. E., Yang, Q., Le Jeune, B., & Herve, Y. (1993). Obtention d’embrions par culture d’antheres chez le chou-fleur et le brocoli et evaluation des potentialites du materiel obtenu pour la creation varietale. Agronomie, 13(7), 579–590.

    Article  Google Scholar 

  • Chen, J. L., & Beversdorf, W. D. (1994). A combined use of microprojectile bombardment and DNA imbibition enhances transformation frequency of canola (Brassica napus L.). Theoretical and Applied Genetics, 88(2), 187–192.

    Google Scholar 

  • Chen, J. F., Cui, A., Malik, A., & Mbira, K. G. (2011). In vitro haploid and dihaploid production via unfertilized ovule culture. Plant Cell, Tissue and Organ Culture, 104, 311–319.

    Article  Google Scholar 

  • Chen, X. M., Wang, F. J., Li, S. M., & Zhang, W. X. (2013). Stable production of wheat haploid and doubled haploid by wheat X maize cross. Acta Agronomica Sinica, 39, 2247–2252.

    Article  CAS  Google Scholar 

  • Chen, X., Nilanthi, D., Yang, Y., & Wu, H. (2016). Anther culture and plant regeneration of tetraploid purple coneflower (Echinacea purpurea L.). Journal of Biosciences and Medicines, 4, 89–96.

    Article  CAS  Google Scholar 

  • Christianson, M. L., & Chiscon, M. O. (1978). Use of Haploid Plants as Bioassays for Mutagens. Environmental Health Perspectives, 27, 77–83.

    PubMed  PubMed Central  CAS  Google Scholar 

  • Chu, C. C., Hill, R. D., & Brule-Babel, A. L. (1990). High frequency of pollen embryoid formation and plant regeneration in Triticum aestivum L. on monosaccharide containing media. Plant Science, 66, 255–262.

    Article  CAS  Google Scholar 

  • Chugh, A., Amundsen, E., & Eudes, F. (2009). Translocation of cell-penetrating peptides and delivery of their cargoes in triticale microspores. Plant Cell Reports, 28(5), 801–810.

    Article  PubMed  CAS  Google Scholar 

  • Chupeau, Y., Caboche, M., & Henry, Y. (Eds.). (1998). Androgenesis and haploid plants. Berlin: Springer.

    Google Scholar 

  • Chuong, P. V., Beyersdorf, W. D., Powell, A. D., & Pauls, K. P. (1988). Somatic transfer of cytoplasmic traits in Brassica napus L. by haploid protoplast fusion. MGG Molecular & General Genetics, 211(2), 197–201.

    Google Scholar 

  • Custers, J. B. M., Cordewener, J. H. G., Nollen, Y., Dons, J. J., & van Lookeren-Campagne, M. M. (1994). Temperature controls both gametophytic and sporophytic development in microspore cultures of Brassica napus. Plant Cell Reports, 13, 267–271.

    Article  PubMed  CAS  Google Scholar 

  • Dahleen, L. S. (1999). Donor-plant environment effects on regeneration from barley embryo-derived callus. Crop Science, 39, 682–685.

    Article  Google Scholar 

  • Dal, B., Sari, N., & Solmaz, İ. (2016). Effect of different irradiation sources and doses on haploid embryo induction in Altinbas (Cucumis melo L. var. inodorus) melons. Turkish Journal of Agriculture and Forestry, 40, 552–559.

    Article  CAS  Google Scholar 

  • Delourme, R., Falentin, C., Fomeju, B., Boillot, M., Lassalle, G., André, I., Duarte, J., Gauthier, V., Lucante, N., Marty, A., Pauchon, M., Pichon, J.-P., Ribière, N., Trotoux, G., Blanchard, P., Rivière, N., Martinant, J.-P., & Pauquet, J. (2013). High-density SNP-based genetic map development and linkage disequilibrium assessment in Brassica napus L. BMC Genomics, 14(1), 120

    Google Scholar 

  • Dimitrova, A. P., & Christov, A. M. (1992). Electrically induced protoplast fusion using pulse electric fields for dieelectrophoresis. Plant Physiology, 100, 2008–2012.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Doi, H., Takahashi, R., Hikage, T., & Takahat, Y. (2010). Embryogenesis and doubled haploid production from anther culture in gentian (Gentiana triflora). Plant Cell, Tissue and Organ Culture, 102(1), 27–33.

    Article  Google Scholar 

  • Doi, H., Yokoi, S., Hikage, T., Nishihara, M., Tsutsumi, K., & Takahata, Y. (2011). Gynogenesis in gentians (Gentiana triflora, G. scabra): Production of haploids and doubled haploids. Plant Cell Reports, 30, 1099–1106.

    Article  PubMed  CAS  Google Scholar 

  • Doi, H., Hosh, N., Yamada, E., Yokoi, S., Nishihara, M., Hikage, T., & Takahata, Y. (2013). Efficient haploid and doubled haploid production from unfertilized ovule culture of gentians (Gentiana spp.). Breeding Science, 63, 400–406.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Dormann, M., Wang, H.M., Oelck, M. (2001) Transformed embryogenic microspores for the generation of fertile homozygous plants. USA Patent US 6,316,694 B1

    Google Scholar 

  • Driks, R., et al. (2009). Reverse breeding: A novel breeding approach based on engineered meiosis. Plant Biotechnology Journal, 7(9), 837–845.

    Article  CAS  Google Scholar 

  • Duke, J. A. (2001). Handbook of phytochemical constituents of GRAS herbs and other economic plants. New York: CRC Press.

    Google Scholar 

  • Dunwell, J. M. (1986). Pollen, ovule and embryo culture, as tools in plant breeding. In L. A. Withers & P. G. Alderson (Eds.), Plant tissue culture and its agricultural applications (pp. 375–404). London: Butterworths.

    Chapter  Google Scholar 

  • Dunwell, J. M. (2010). Haploids in flowering plants: Origins and exploitation. Plant Biotechnology Journal, 8, 377–424.

    Article  PubMed  CAS  Google Scholar 

  • Eshaghi, Z. C., Abdollahi, M. R., Moosavi, S. S., Deljou, A., & Seguí-Simarro, J. M. (2015). Induction of androgenesis and production of haploid embryos in anther cultures of borage (Borago officinalis L.). Plant Cell, Tissue and Organ Culture, 122, 321–329.

    Article  CAS  Google Scholar 

  • Eudes, F., Shim, Y. S., & Jiang, F. (2014). Engineering the haploid genome of microspores. Biocatalysis and Agricultural Biotechnology, 3, 20–23.

    Article  Google Scholar 

  • Fabijanski, S. F., Altosaar, I., & Arnison, P. G. (1991). Heat shock response during anther culture of broccoli (Brassica oleracea var italica). Plant Cell Tissue and Organ Culture, 26, 203–212.

    Google Scholar 

  • Fan, Z., Armstrong, K. C., & Keller, W. A. (1998). Development of microspores in vivo and in vitro in Brassica napus L. Protoplasma, 147, 191–199.

    Article  Google Scholar 

  • Ferrie, A. M. R. (2006). Doubled haploid production in nutraceutical species: A review. Euphytica, 158, 347–357.

    Article  Google Scholar 

  • Ferrie, A. M. R., & Caswell, K. L. (2011). Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell, Tissue and Organ Culture, 104, 301–309.

    Article  Google Scholar 

  • Ferrie, A. M. R., & Keller, W. A. (1995). Microspore culture for haploid plant production. In O. L. Gamborg & G. C. Phillips (Eds.), Plant cell, tissue and organ culture. Fundamental methods (pp. 155–164). Berlin: Springer.

    Chapter  Google Scholar 

  • Ferrie, A. M. R., Bethune, T., & Kernan, Z. (2005). An overview of preliminary studies on the development of doubled haploid protocols for nutraceutical species. Acta Physiologiae Plantarum, 27, 735–741.

    Article  Google Scholar 

  • Ferrie, M. R. A. (2013). Advances in microspore culture technology: A biotechnological tool for the improvement of medicinal plants. In Biotechnology for Medicinal Plants: Micropropagation and Improvement (pp. 191–206). Berlin/Heidelberg: Springer. https://doi.org/10.1007/978-3-642-29974-2_8.

  • Flachsland, E., Mroginski, L., & Davina, J. (1996). Regeneration of plants from anthers of Stevia rebaudiana Bertoni (Compositae) cultivated in vitro. Biocell, 20, 87–90.

    CAS  Google Scholar 

  • Foroughi-Wehr, B., & Wenzel, G. (1993). Androgenesis and parthenogenesis. In W. D. Hayward, N. O. Bosemark, & I. Romagosa (Eds.), Plant breeding: Principles and prospects (pp. 261–277). London: Chapman and Hall.

    Chapter  Google Scholar 

  • Forster, B. P., & Thomas, W. T. (2005). Doubled haploids in genetics and plant breeding. Plant Breeding Reviews, 25, 57–88.

    CAS  Google Scholar 

  • Fukuoka, H., Ogawa, T., Matsuoka, M., Ohkawa, Y., & Yano, H. (1998). Direct gene delivery into isolated microspores of rapeseed (Brassica napus L.) and the production of fertile transgenic plants. Plant Cell Reports, 17(5), 323–328.

    Google Scholar 

  • Geoffriaeu, E., Kahane, R., & Rancillac, M. (1997). Variation of gynogenesis ability in onion (Allium cepa L). Euphytica, 94, 37–44.

    Article  Google Scholar 

  • Germanà, M. A. (1997). Haploidy in Citrus. In S. M. Jain, S. K. Sopory, & R. E. Veilleux (Eds.), In vitro haploid production in higher plants (Vol. 5, pp. 195–217). Dordrecht: Kluwer.

    Chapter  Google Scholar 

  • Germanà, M. A. (2006). Doubled haploid production in fruit crops. Plant Cell Tissue Organ, 86, 131–146.

    Article  Google Scholar 

  • Germanà, M. A. (2007). Haploidy. In I. Khan (Ed.), Citrus. Genetics, breeding and biotechnology (pp. 167–196). Wallingford: CABI.

    Chapter  Google Scholar 

  • Germanà, M. A. (2009). Haploid and doubled haploids in fruit trees. In A. Touraev, B. Forster, & M. Jain (Eds.), Advances in haploid production in higher plants (pp. 241–263). Heidelberg: Springer.

    Chapter  Google Scholar 

  • Gilani, A. H., Shaheen, F., Saeed, S. A., Bibi, S., Irfanullah, S. M., & Faizi, S. (2000). Hypotensive action of coumarin glycosides from Daucus carota. Phytomedicine, 7(5), 423–426.

    Article  PubMed  CAS  Google Scholar 

  • Gilania, A. H., Bashira, S., & Khan, A. (2007). Pharmacological basis for the use of Borago officinalis in gastrointestinal, respiratory and cardiovascular disorders. Journal of Ethnopharmacology, 114(3), 399.

    Google Scholar 

  • Gao, Z.-Y., Zhao, S.-C., He, W.-M., Guo, L.-B., Peng, Y.-L., Wang, J.-J., Guo, X.-S., Zhang, X.-M., Rao, Y.-C., Zhang, C., Dong, G.-J., Zheng, F.-Y., Lu, C.-X., Hu, J., Zhou, Q., Liu, H.-J., Wu, H.-Y., Xu, J., Ni, P.-X., Zeng, D.-L., Liu, D.-H., Tian, P., Gong, L.-H., Ye, C., Zhang, G.-H., Wang, J., Tian, F.-K., Xue, D.-W., Liao, Y., Zhu, L., Chen, M.-S., Li, J.-Y., Cheng, S.-H., Zhang, G.-Y., Wang, J., & Qian, Q. (2013). Dissecting yield-associated loci in super hybrid rice by resequencing recombinant inbred lines and improving parental genome sequences. Proceedings of the National Academy of Sciences, 110(35), 14492–14497.

    Google Scholar 

  • Grosser, J. W. (1994). In vitro culture of tropical fruits. In I. K. Vasil & T. A. Thorpe (Eds.), Plant cell tissue culture 20 (pp. 475–496). Dordrecht: Kluwer Academic Publishers.

    Google Scholar 

  • Guerche, P., Charbonnier, M., Jouanin, L., Tourneur, C., Paszkowski, J., & Pelletier, G. (1987). Direct gene transfer by electroporation in Brassica napus. Plant Science, 52(1–2), 111–116.

    Google Scholar 

  • Guha, S., & Maheshwari, S. C. (1964). In vitro production of embryos from anthers of Datura. Nature, 204, 497.

    Article  Google Scholar 

  • Guha, S., & Maheshwari, S. C. (1966). Cell division and differentiation of embryos in the pollen grains of Datura in vitro. Nature, 212, 97–98.

    Article  Google Scholar 

  • Gurel, S., Gurel, E., & Kaya, Z. (2000). Doubled haploid plant production from unpollinated ovules of sugar beet (Beta vulgaris L.). Plant Cell Reports, 19, 1155–1159.

    Article  CAS  PubMed  Google Scholar 

  • Gurushidze, M., Hensel, G., Hiekel, S., Schedel, S., Valkov, V., & Kumlehn, J. (2014). True-breeding targeted gene knock-out in barley using designer TALE-nuclease in haploid cells. PLoS One, 9, 1–9.

    Article  CAS  Google Scholar 

  • Harbige, L. S., Layward, L., Downes, M. M., Dumonde, D. C., & Amor, S. (2000). The protective effects of omega-6 fatty acids in experimental autoimmune encephalomyelitis (EAE) in relation to transforming growth factor-beta 1 (TGF-1) upregulation and increased prostaglandin E2 (PGE2) production. Clinical and Experimental Immunology, 122(3), 445–452.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Harborne, J. B., & Williams, C. A. (2004). Phytochemistry of the genus Echinacea. In S. Miller (Ed.), Echinacea. The genus Echinacea (pp. 55–71). Boca Raton: CRC Press.

    Chapter  Google Scholar 

  • Harvey, A. L. (2008). Natural products in drug discovery. Drug Discovery Today, 13, 894–901.

    Article  PubMed  CAS  Google Scholar 

  • He, M., Nichterlein, K., van Zanten, L., & Ahloowalia, B. S. (2000). Officially released mutant varieties – The FAO/IAEA database. Mutation Breed Review, 12, 1–84.

    CAS  Google Scholar 

  • Henikoff, S., & Dalal, Y. (2005). Centromeric Chromatin: What makes it unique? Current Opinion in Genetics and Development, 15(2), 177–184.

    Article  PubMed  CAS  Google Scholar 

  • Hobbs, C. R. (1998). The Echinacea handbook. Capitola: Botanica.

    Google Scholar 

  • Hofinger, B. J., Ankele, E., Gülly, C., Heberle-Bors, E., & Pfosser, M. F. (2000). The involvement of the plastid genome in albino plant regeneration from microspores in wheat. In B. Bohanec (Ed.), Biotechnological approaches for utilization of gametic cells-COST 824 (pp. 215–228). Luxembourg: OP-EUR.

    Google Scholar 

  • Horner, M., & Pratt, M. L. (1979). Amino acid analysis of in vivo and androgenic anthers of Nicotiana tabacum. Protoplasma, 98(3), 279–282.

    Article  CAS  Google Scholar 

  • Horner, M., & Street, H. E. (1978). Problems encountered in the culture of isolated pollen of a Burley cultivar of Nicotiana tabacum. Journal of Experimental Botany, 29(108), 217–226.

    Article  Google Scholar 

  • Horrobin, D. F., Stewart, C., Carmichael, H., & Jamal, G. C. (1993). Use of gamma-linolenic acid and related compounds for the manufacture of a medicament for the treatment of complications of diabetes mellitus. European Patent 0,218,460.

    Google Scholar 

  • Hosp, J., et al. (2007). Transcriptional and metabolic profiles of stress-induced, embryogenic tobacco microspores. Plant Molecular Biology, 63, 137–149.

    Article  PubMed  CAS  Google Scholar 

  • Hoveida, Z. S., et al. (2017). Production of doubled haploid plants from anther cultures of borage (Borago officinalis L.) by the application of chemical and physical stress. Plant Cell, Tissue and Organ Culture, 130(2), 369–378.

    Article  CAS  Google Scholar 

  • Hu, H., & Yang, H. Y. (Eds.). (1986). Haploids in higher plants in vitro. Beijing/Berlin: China Academic Publishers/Springer.

    Google Scholar 

  • Huang, B. (1992). Genetic manipulation of microspores and microspore-derived embryos. Vitro Cellular & Developmental Biology – Plant, 28(2), 53–58.

    Google Scholar 

  • Immonen, S., & Anttila, H. (1998). Impact of microspore developmental stage on induction and plant regeneration in rye anther culture. Plant Science, 139(2), 213–222.

    Article  CAS  Google Scholar 

  • Indrianto, A., Heberle-Bors, E., & Tourae, A. (1999). Assessment of various stresses and carbohydrates for their effect on the induction of embryogenesis in isolated wheat microspores. Plant Science, 143(1), 71–79.

    Article  CAS  Google Scholar 

  • Irikova, T., Grozeva, S., & Rodeva, V. (2011). Anther culture in pepper (Capsicum annuum L.) in vitro. Acta Physiologiae Plantarum, 33, 1559–1570.

    Article  CAS  Google Scholar 

  • Jain, S. M., Sopory, S. K., & Veilleux, R. E. (Eds.). (1996–1997). In vitro haploid production in higher plants (Vol. 1–5). Dordrecht: Kluwer.

    Google Scholar 

  • Jin, W., Melo, J. R., Nagaki, K., Talbert, P. B., Henikoff, S., Dawe, R. K., & Jiang, J. (2004). Maize centromeres: Organization and functional adaptation in the genetic background of oat. The Plant Cell, 16, 571–581.

    Google Scholar 

  • Jardinaud, M.-F., Souvré, A., & Alibert, G. (1993). Transient GUS gene expression in Brassica napus electroporated microspores. Plant Science, 93(1–2), 177–184.

    Google Scholar 

  • Javornik, B., Bohanec, B., & Campion, B. (1998). Second cycle gynogenesis in onion, Allium cepa L, and genetic analysis of the plants. Plant Breeding, 117(3), 275–278.

    Article  Google Scholar 

  • Jogdand, S. N. (2001). Protoplast technology, gene biotechnology (3rd ed.pp. 171–186). New Delhi: Himalaya Publishing House.

    Google Scholar 

  • Joosen, R., et al. (2007). Combined transcriptome and proteome analysis identifies pathways and markers associated with the establishment of rapeseed microspore-derived embryo development. Plant Physiology, 144, 155–172.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Jones-Villeneuve, E., Huang, B., Prudhomme, I., Bird, S., Kemble, R., Hattori, J., & Miki, B. (1995). Assessment of microinjection for introducing DNA into uninuclear microspores of rapeseed. Plant Cell, Tissue and Organ Culture, 40(1), 97–100.

    Google Scholar 

  • Jumin, H. B., & Nito, N. (1996). Plant regeneration via somatic embryogenesis from protoplasts of six plant species related to citrus. Plant Cell Reports, 15, 332–336.

    Article  PubMed  CAS  Google Scholar 

  • Kasha, K. J. (1974). Haploids from somatic cells. In K. J. Kasha (Ed.), Haploids in higher plants: Advances and potential (pp. 67–87). Ontario: University of Guelph.

    Google Scholar 

  • Kasha, K. J., & Kao, K. N. (1970). High frequency haploid production in barley (Hordeum vulgare L.). Nature, 225, 874–876.

    Article  PubMed  CAS  Google Scholar 

  • Kasha, K. J., Simion, E., Oro, R., & Shim, Y. S. (2003). Barley isolated microspore culture protocol. In M. Maluszynski, K. J. Kasha, B. P. Forster, & I. Szarejko (Eds.), Doubled haploid production in crop plants. Dordrecht: Springer.

    Chapter  Google Scholar 

  • Kast, R. E. (2001). Borage oil reduction of rheumatoid arthritis activity may be mediated by increased cAMP that suppresses tumor necrosis factor-alpha. International Immuno Pharmacology, 1(12), 2197–2199.

    Article  CAS  Google Scholar 

  • Kelliher, T., et al. (2016). Maternal haploids are preferentially induced by CENH3- tail swap transgenic complementation in maize. Frontiers in Plant Science, 7, 414. https://doi.org/10.3389/fpls.2016.00414.

    Article  PubMed  PubMed Central  Google Scholar 

  • Kernan, Z., & Ferrie, A. M. R. (2006). Microspore embryogenesis and the development of a double haploidy protocol for cow cockle (Saponaria vaccaria). Plant Cell Reports, 25, 274–280.

    Article  PubMed  CAS  Google Scholar 

  • Kiełkowska, A., & Adamus, A. (2010). In vitro culture of unfertilized ovules in carrot (Daucus carota L.). Plant Cell, Tissue and Organ Culture, 102, 309–319.

    Article  Google Scholar 

  • Kiełkowska, A., Adamus, A., & Baranski, R. (2014). An improved protocol for carrot haploid and doubled haploid plant production using induced parthenogenesis and ovule excision in vitro. In Vitro Cellular & Developmental Biology – Plant, 50, 376–383.

    Google Scholar 

  • King, R. M., & Robinson, H. (1987). The genera of the Eupatoriae (Asteraceae) (pp. 1–180). Saint Louis: Missouri Botanical Garden Library.

    Google Scholar 

  • Kinghorn, A. D., & Soejarto, D. D. (1985). Current status of stevioside as a sweetening agent for human use. In H. Wagner, H. Hikino, & N. R. Farnsworth (Eds.), Economic and medicinal plant research (Vol. 1, pp. 1–52). London: Academic.

    Google Scholar 

  • Kiszczak, W., Kowalska, U., Kapuścińska, A., Burian, M., & Górecka, K. (2017). Comparison of methods for obtaining doubled haploids of carrot. Acta Societatis Botanicorum Poloniae, 86(2), 3547.

    Article  Google Scholar 

  • Kowalska, U., Rybaczek, D., Krzyżanowska, D., Kiszczak, W., & Górecka, K. (2008). Cytological assessment of carrot plants obtained in anther culture. Acta Biologica Cracoviensia Series Botanica, 50(2), 7–11.

    Google Scholar 

  • Kumar, G. H., Chandra Mohan, K. V. P., Rao, A. J., & Nagini, S. (2009). Investigational Nimbolide a limonoid from Azadirachta indica inhibits proliferation and induces apoptosis of human choriocarcinoma (BeWo) cells. Investigational New Drugs, 27(3), 246–252.

    Article  CAS  Google Scholar 

  • Kurtar, E. S., Sarı, N., & Abak, K. (2002). Obtention of haploid embryos and plants through irradiated pollen technique in squash (Cucurbita pepo L.). Euphytica, 127, 335.

    Article  CAS  Google Scholar 

  • Kyo, M., Hattori, S., Yamaji, N., Pechan, P., & Fukui, H. (2003). Cloning and characterization of cDNAs associated with the embryogenic dedifferentiation of tobacco immature pollen grains. Plant Science, 164, 1057–1066.

    Article  CAS  Google Scholar 

  • Lantos, C., et al. (2009). Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via coculture with ovary of pepper or wheat. Plant Cell, Tissue and Organ Culture, 97, 285–293.

    Article  Google Scholar 

  • Leach, C. R., Mayo, O., & Burger, R. (1990). Quantitatively determined self-incompatibility Outcrossing in Borago officinalis. Theoretical and Applied Genetics, 79, 427–430.

    Article  PubMed  CAS  Google Scholar 

  • Li, T. C. S. (1998). Echinacea: Cultivation and medicinal value. Hort Technology, 8, 122–129.

    Google Scholar 

  • Li, J. R., Zhuang, F. Y., Ou, C. G., Hu, H., Zhao, Z. W., & Mao, J. H. (2013). Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.). Plant Cell, Tissue and Organ Culture, 112, 275–287.

    Article  Google Scholar 

  • Lian, L. H., et al. (2010). Anti-apoptotic activity of gentiopicroside in D galactosamine/lipopolysaccharide-induced murine fulminant hepatic failure. Chemico-Biological Interactions, 188, 127–133.

    Article  PubMed  CAS  Google Scholar 

  • Liu, W. (2009, October). Production of doubled haploid transgenic Wheat (Triticum aestivum L.): Transformation of microspores and regeneration of homozygous transformants. Saarbrücken: VDM Verlag.

    Google Scholar 

  • Liu, G. S., Li, Y., Liu, F., & Cao, M. Q. (1995). The influence of high temperature on the cultures of isolated microspores of Chinese cabbage. Acta Botanica Sinica, 37, 140–146.

    Google Scholar 

  • Liu, W., Zheng, Y., Polle, E., & Konzak, C. F. (2002). Highly efficient doubled-haploid production in wheat (Triticum aestivum L.) via induced microspore embryogenesis. Crop Sci, 42, 686−692.

    Google Scholar 

  • Lux, H., Herrmann, L., & Wetzel, C. (1990). Production of haploid sugar-beet (Beta vulgaris L.) by culturing unpollinated ovules. Plant Breeding, 104, 177–183.

    Article  Google Scholar 

  • Magoon, M. L., & Khanna, K. R. (1963). Haploids. Caryologia, 16, 191–235.

    Article  Google Scholar 

  • Malik, M. R., Wang, F., Dirpaul, J. M., Zhou, N., Polowick, P. L., Ferrie, A. M. R., & Krochko, J. E. (2007). Transcript profiling and identification of molecular markers for early microspore embryogenesis in Brassica napus. Plant Physiology, 144, 134–154.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Maluszynski, M., Kasha, K. J., Forster, B. P., & Szarejko, I. (Eds.). (2003a). Doubled haploid production in crop plants: A manual. Dordrecht: Kluwer.

    Google Scholar 

  • Maluszynski, M., Kasha, K. J., & Szarejko, I. (2003b). Published double haploid protocols in plant species (M. Maluszynski, K. J. Kasha, Eds., pp. 397–403). Dordrecht: Kluwer Academic Publishers.

    Google Scholar 

  • Manaharana, T., Tenga, L. L., Appletonb, D., Minga, C. H., Masilamanic, T., & Palanisamyd, U. D. (2011). Antioxidant and antiglycemic potential of Peltophorum pterocarpum plant parts. Food Chemistry, 129, 1355–1361.

    Article  CAS  Google Scholar 

  • Maraschin, S. F., de Priester, W., Spaink, H. P., & Wang, M. (2005). Androgenic switch: An example of plant embryogenesis from the male gametophyte perspective. Journal of Experimental Botany, 56, 1711–1726.

    Article  PubMed  CAS  Google Scholar 

  • Maraschin, S. F., Caspers, M., Potokina, E., Wülfert, F., Graner, A., Spaink, H. P., & Wang, M. (2006). cDNA array analysis of stress-induced gene expression in barley androgenesis. Physiologia Plantarum, 127, 535–550.

    Article  CAS  Google Scholar 

  • Martinez, L. E., Aguero, C. B., Lopez, M. E., & Galmarini, C. R. (2000). Improvement of in vitro gynogenesis induction in onion (Allium cepa L.) using polyamines. Plant Science, 156, 221–226. https://doi.org/10.1016/S0168-9452(00)00263-6.

    Article  PubMed  CAS  Google Scholar 

  • Matsubara, S., Dohya, N., Murakami, K., Nishio, T., & Dore, C. (1995). Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore cultures. Acta Horticulturae, 392, 129–137.

    Article  Google Scholar 

  • Melchers, G. (1978). Potatoes for combined somatic and sexual breeding methods; plants from protoplasts and fusion of protoplasts of potato and tomato. In A. W. Alfermann & E. Reinhard (Eds.), Production of natural compounds by cell culture methods: Proceedings of an International Symposium, on plant cell culture (pp. 306–311). Mfinchen: Ges. f. Strahlen- und Umweltforschung.

    Google Scholar 

  • Metwally, E. I., Moustafa, S. A., Sawy, B. I. E. L., Haroun, S. A., & Shalaby, T. A. (1998). Production of haploid plants from in vitro culture of unpollinated ovules of Cucurbita pepo. Plant Cell, Tissue and Organ Culture, 52, 117–121.

    Article  CAS  Google Scholar 

  • Michelmore, R. W., Paran, I., & Kesseli, R. V. (1991). Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences, 88(21), 9828–9832

    Google Scholar 

  • Musial, K., Bohanec, B., Jakše, M., & Przywara, L. (2005). The development of onion (Allium cepa L.) Embryo sacs in vitro and gynogenesis induction in relation to flower size. In Vitro Cellular & Developmental Biology – Plant, 41(4), 446–452.

    Google Scholar 

  • Murch, S. J., Peiris, S. E., Shi, W. L., Zobayed, S. M. A., & Saxena, P. K. (2006). Genetic diversity in seed populations of Echinacea purpurea controls the capacity for regeneration, route of morphogenesis and phytochemical composition. Plant Cell Reports, 25, 522–532.

    Article  PubMed  CAS  Google Scholar 

  • Narayanswamy, S. (1994). Plant cells and tissue cultures. Plant protoplast: Isolation, culture and fusion (pp. 391–469). New Delhi: TATA MC Graw Hill Publishing Company.

    Google Scholar 

  • Nat, J. V., Sluis, W. G., & Labadie, R. P. (1982). Gentiogenal, a new antimicrobial iridoid derived from gentiopicrin (gentiopicroside). Planta Medica, 45, 161–162. https://doi.org/10.1055/s-2007-971351.

    Article  PubMed  Google Scholar 

  • Nathana, V. K., Antonisamyb, J. M., Gnanarajc, N. E. W., & Subramaniana, K. M. (2012). Phytochemical and bio-efficacy studies on methanolic flower extracts of Peltophorum pterocarpum (DC.) Baker ex Heyne. Asian Pacific Journal of Tropical Biomedicine, 2, 641–645.

    Article  Google Scholar 

  • Nehlin, L., Möllers, C., Bergman, P., & Glimelius, K. (2000). Transient Î2-gus and gfp Gene Expression and Viability Analysis of Microprojectile Bombarded Microspores of Brassica napus L. Journal of Plant Physiology, 156(2), 175–183.

    Google Scholar 

  • Neuhaus, G., Spangenberg, G., Mittelsten Scheid, O., & Schweiger, H.-G. (1987). Transgenic rapeseed plants obtained by the microinjection of DNA into microspore-derived embryoids. Theoretical And Applied Genetics, 75(1).

    Google Scholar 

  • Nitsch, C. (1974). La culture de pollen isolé sur milieu synthétique. C R Acad Sci (Paris), 278, 1031–1034.

    CAS  Google Scholar 

  • Nitsch, C., & Norreel, B. (1973). Effet d'un choc thermique sur le pouvoir embryogène du pollen de Datura innoxia cultivé dans l’anthère ou isolé de l’anthère. C R Acad Sci (Paris), 276, 303–306.

    Google Scholar 

  • Otani, M., Wakita, Y., & Shimada, T. (2005). Doubled haploid plant production of transgenic rice (Oryza sativa L.) using anther culture. Plant Biotechnology, 22(2), 141–143.

    Article  CAS  Google Scholar 

  • Owen, H. R., & Raymond Miller, A. (1996). Haploid plant regeneration from anther cultures of three north american cultivars of strawberry (Fragaria x ananassa Duch.). Plant Cell Reports, 15(12), 905–909.

    Google Scholar 

  • Ozkum, D., & Tipirdamaz, R. (2002). The effects of cold treatment and charcoal on the in vitro androgenesis of pepper (Capsicum annuum L.). Turkish Journal of Botany, 26, 131–139.

    Google Scholar 

  • Palmer, C. E., Keller, W. A., & Kasha, K. J. (Eds.). (2005). Haploids in crop improvement II (Vol. 56). Heidelberg: Springer.

    Google Scholar 

  • Parra-Vega, V., Renau-Morata, B., Sifres, A., & Seguí-Simarro, J. M. (2013). Stress treatments and in vitro culture conditions influence microspore embryogenesis and growth of callus from anther walls of sweet pepper (Capsicum annuum L.). Plant Cell, Tissue and Organ Culture, 112, 353–360.

    Article  CAS  Google Scholar 

  • Pasha, C., Kuhad, R. C., & Rao, C. V. (2007). Strain improvement of thermotolerant Saccharomyces cerevessie VS3 strain for better utilization of lignocellulosic substrates. Journal of Applied Microbiology, 103, 1480–1489.

    Article  PubMed  CAS  Google Scholar 

  • Pathirana, R., Frew, T., Hedderley, D., Timmerman-Vaughan, G., & Morgan, E. R. (2011). Haploid and doubled haploid plants from developing male and female gametes of Gentiana triflora. Plant Cell Reports, 30, 1055–1065.

    Article  PubMed  CAS  Google Scholar 

  • Pechan, P. M., & Keller, W. A. (1988). Identification of potentially embryogenic microspores in Brassica napus. Physiologia Plantarum, 74(2), 377–384.

    Article  Google Scholar 

  • Pechan, P. M. (1989). Successful cocultivation of Brassica napus microspores and proembryos with Agrobacterium. Plant Cell Reports, 8(7), 387–390.

    Google Scholar 

  • Phippen, C. I., & Ockendon, D. J. (1990). Genotype, plant, bud size and factors affecting anther culture of cauliflower (Brassica oleracea var. botrytis). Theoretical and Applied Genetics, 79, 33–38.

    Article  PubMed  CAS  Google Scholar 

  • Piosik, Ł., Zenkteler, E., & Zenkteler, M. (2016). Development of haploid embryos and plants of Lactuca sativa induced by distant pollination with Helianthus annuus and H. tuberosus. Euphytica, 208, 439–451.

    Article  CAS  Google Scholar 

  • Pons, T. L. (1992). Seed responses to light. In M. Fenner (Ed.), Seeds: The ecology of regeneration in plants (pp. 259–284). Wallingford: CAB International.

    Google Scholar 

  • Rao, P. V. L., & De, D. N. (1987). Haploid plants from in vitro anther culture of the leguminous tree, Peltophorum pterocarpum (DC) K. Hayne (Copper pod). Plant Cell, Tissue and Organ Culture, 11, 167–177.

    Article  Google Scholar 

  • Raquin, C. (1983). Utilization of different sugars as carbon sources for in vitro cultures of Petunia. Z Pflanzenphysol, 111, 453–457.

    Article  CAS  Google Scholar 

  • Ravi, M., & Chan, S. W. L. (2010). Haploid plants produced by centromere-mediated genome elimination. Nature, 464, 615–618.

    Article  PubMed  CAS  Google Scholar 

  • Ravi, M., et al. (2014). A powerful haploid tool for plant genetics. Nature Methods, 12, 15.

    Google Scholar 

  • Reiss, E., Schubert, J., Scholze, P., Krämer, R., & Sonntag, K. (2009). The barley thaumatin-like protein Hv-TLP8 enhances resistance of oilseed rape plants to. Plant Breeding, 128(2), 210–212.

    Google Scholar 

  • Reynolds, T. L. (1997). Pollen embryogenesis. Plant Molecular Biology, 33, 1–10.

    Article  PubMed  CAS  Google Scholar 

  • Rossi, P. G., Bao, L., et al. (2007). (E)-Methylisoeugenol and elemicin: Antibacterial components of Daucus carota L. Essential oil against Campylobacter jejuni Rossi. Journal of Agricultural and Food Chemistry, 55(18), 7332–7336.

    Article  PubMed  CAS  Google Scholar 

  • Rudolf, K., Bohanec, B., & Hansen, M. (1999). Microspore culture of white cabbage, Brassica oleracea var. capitata L.: Genetic improvement of non-responsive cultivars and effect of genome doubling agents. Plant Breeding, 118, 237–241.

    Article  CAS  Google Scholar 

  • Sanei, M., Pickering, R., Kumke, K., Nasuda, S., & Houben, A. (2011). Loss of centromeric histone H3 (CENH3) from centromeres precedes uniparental chromosome elimination in interspecific barley hybrids. Proceedings of the National Academy of Sciences of the United States of America, 108, 498–505.

    Article  Google Scholar 

  • Sannemann, W., Huang, B. E., Mathew, B., & Léon, J. (2015). Multi-parent advanced generation inter-cross in barley: High-resolution quantitative trait locus mapping for flowering time as a proof of concept. Molecular Breeding, 35(3)

    Google Scholar 

  • Sauton, A. (1989). Haploid gynogenesis in Cucumis sativus induced by irradiated pollen. Cucurbit Genetics Cooperative Report, 12, 22–23.

    Google Scholar 

  • Seguí-Simarro, J. M. (2010). Androgenesis revisited. The Botanical Review, 76, 377–404.

    Article  Google Scholar 

  • Seguí-Simarro, J. M., & Nuez, F. (2008). How microspores transform into haploid embryos: Changes associated with embryogenesis induction and microspore derived embryogenesis. Physiologia Plantarum, 134, 1–12.

    Article  PubMed  CAS  Google Scholar 

  • Sejdler, L. K., & Dabrowska, J. (1996). Studies on the biology of flowering and fruiting of purple coneflower (Echinacea purpurea Moench). Pt. 1. Biology of flowering and fruiting. Herba Polonica, 42, 83–87.

    Google Scholar 

  • Shariatpanahi, M. E., Bal, U., Heberle-Bors, E., & Touraev, A. (2006). Stresses applied for the re-programming of plant microspores towards in vitro embryogenesis. Physiologia Plantarum, 127, 519–534.

    Article  CAS  Google Scholar 

  • Sharma, K. D., Karki, S., Thakur, N. S., & Attri, U. (2012). Chemical composition, functional properties and processing of carrot—a review. Journal of Food Science and Technology, 49(1), 22–32.

    Article  PubMed  CAS  Google Scholar 

  • Sibi, M. L., Kobaissi, A., & Shekafandeh, A. (2001). Green haploid plants from unpollinated ovary culture in tetraploid wheat (Triticum durum Defs.). Euphytica, 122, 351–359.

    Article  Google Scholar 

  • Simon, P. W., Freeman, R. E., Vieira, J. V., Boiteux, L. S., Briard, M., Nothnagel, T., Michalik, B., & Kwon, Y. (2008). Carrot. In J. Prohens & F. Nuez (Eds.), Handbook of plant breeding (Vol. 2, pp. 327–357). New York: Springer.

    Google Scholar 

  • Sluis, W. G., & Labadie, R. P. (1981). Fungitoxic activity of the secoiridoid glucoside gentiopicrin (gentiopicroside). Planta Medica, 42, 139–140.

    Article  PubMed  Google Scholar 

  • Smykal, P. (2000). Pollen embryogenesis – The stress mediated switch from gametophytic to sporophytic development. Current status and future prospects. Biologia Plantarum, 43, 481–489.

    Article  CAS  Google Scholar 

  • Srinivas, R., & Panda, T. (1997). Localization of carboxymethyl cellulase in the intergeneric fusants of Trichodermma reesei QM 9414 and Saccharomyces cerevisee NCIM 3288. Bioprocess and Biosystems Engineering, 18, 71–73.

    Google Scholar 

  • Staniaszek, M., & Habdas, H. (2006). RAPD technique application for intraline evaluation of androgenic carrot plants. Folia Hortic, 18, 87–97.

    Google Scholar 

  • Sukumaran, S., et al. (2011). Phytochemical constituents and antibacterial efficacy of the flowers of Peltophorum pterocarpum (DC.) Baker ex Heyne. Asian Pacific Journal of Tropical Medicine, 735–738.

    Google Scholar 

  • Sun, T., Simon, P. W., & Tanumihardjo, S. A. (2009). Antioxidant phytochemicals and antioxidant capacity of biofortified carrots (Daucus carota L.) of various colors. Journal of Agricultural and Food Chemistry, 57(10), 4142–4147.

    Article  PubMed  CAS  Google Scholar 

  • Swanson, E. B., & Erickson, L. R. (1989). Haploid transformation in Brassica napus using an octopine-producing strain of Agrobacterium tumefaciens. Theoretical and Applied Genetics, 78(6), 831–835.

    Google Scholar 

  • Szarejko, I., & Forster, B. P. (2007). Doubled haploidy and induced mutation. Euphytica, 158(3), 359–370.

    Article  Google Scholar 

  • Takahata, Y., & Keller, W. A. (1991). High frequency embryogenesis and plant regeneration in isolated microspore culture of Brassica oleracea L. Plant Sci., 74, 235–242.

    Google Scholar 

  • Tavares, A. C., Gonçalves, M. J., Cavaleiro, C., Cruz, M. T., Lopes, M. C., Canhoto, J., & Salgueiro, L. R. (2008). Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activity and cytotoxicity. Journal of Ethnopharmacology, 119(1, 29–134.

    Google Scholar 

  • Telmer, C. A., Newcomb, W., & Simmonds, D. H. (1995). Cellular changes during heat shock induction and embryo development of cultured microspores of Brassica napus cv. Topas. Protoplasma, 185, 106.

    Article  Google Scholar 

  • Tipirdamaz, R., & Ellialtioğlu, S. (1998). The effects of cold treatments and activated charcoal on ABA contents of anthers and in vitro androgenesis in eggplant (Solanum melongena L.). In I. Tsekos & M. Moustakas (Eds.), Progress in botanical research, Proceedings of the 1st Balkan botanical congress. Dordrecht: Kluwer Academic Publishers.

    Google Scholar 

  • Tomiczak, K., Sliwinska, E., & Rybczyński, J. J. (2017). Protoplast fusion in the genus Gentiana: Genomic composition and genetic stability of somatic hybrids between Gentiana kurroo Royle and G. cruciata L. Plant Cell, Tissue and Organ Culture, 131, 1–14. https://doi.org/10.1007/s11240-017-1256-x.

    Article  CAS  Google Scholar 

  • Tosca, A., Arcara, L., & Frangi, P. (1999, October). Effect of genotype and season on gynogenesis efficiency in Gerbera. Plant Cell, Tissue and Organ Culture, 59, 77.

    Article  Google Scholar 

  • Touraev, A., Ilham, A., Vicente, O., & Heberle-Bors, E. (1996a). Stress as the major signal controlling the developmental of tobacco microspores: Towards a unified model of induction of microspore/pollen embriogenesis. Planta, 200, 144–152.

    Article  CAS  Google Scholar 

  • Touraev, A., Indrianto, A., Wratschko, I., Ilham, A., Vicente, O., & Heberle-Bors, E. (1996b). Efficient microspore embryogenesis in wheat (Triticum aestivum L.) induced by starvation at high temperature. Sexual Plant Reproduction, 9, 209–215.

    Article  Google Scholar 

  • Touraev, A., Ilham, A., Vicente, O., & Heberle-Bors, E. (1996c). Stress-induced microspore embryogenesis in tobacco: An optimized system for molecular studies. Plant Cell Reports, 15, 561–565.

    Article  PubMed  CAS  Google Scholar 

  • Touraev, A., Pfosser, M., & Heberle-Bors, E. (2001). The microspore: A haploid multipurpose cell. Advances in Botanical Research, 35, 53–109.

    Article  Google Scholar 

  • Touraev, A., Forster, B. P., & Jain, S. M. (Eds.). (2009). Advances in haploid production in higher plants. Berlin: Springer.

    Google Scholar 

  • Tsuwamoto, R., Fukuoka, H., & Takahata, Y. (2007). Identification and characterization of genes expressed in early embryogenesis from microspores of Brassica napus. Planta, 225, 641–652.

    Article  PubMed  CAS  Google Scholar 

  • Tu, Y., Sun, J., Liu, Y., Ge, X., Zhao, Z., Yao, X., & Zaiyun, L. (2008). Production and characterization of intertribal somatic hybrids of Raphanus sativus and Brassica rapa with dye and medicinal plant Isatis indigotica. Plant Cell Reports, 27(5), 873–883.

    Article  PubMed  CAS  Google Scholar 

  • Tuvesson, S., Dayteg, C., Hagberg, P., Manninen, O., Tanhuanpää, P., Tenhola-Roininen, T., Kiviharju, E., Weyen, J., Förster, J., Schondelmaier, J., Lafferty, J., Marn, M., & Fleck, A. (2007). Molecular markers and doubled haploids in European plant breeding programmes. Euphytica, 158(3), 305–312

    Google Scholar 

  • Tyukavin, G. B., Shmykova, N. A., & Monakhova, M. A. (1999). Cytological study of embryogenesis in cultured carrot anthers. Russian Journal of Plant Physiology, 46(6), 767–773.

    CAS  Google Scholar 

  • Ushijima, S., Nakadai, T., & Uchida, K. (1991). Interspecific electrofusion of protoplasts between Aspergillus oryzae and Aspergillus sojae. Agricultural and Biological Chemistry, 55, 129–136.

    CAS  Google Scholar 

  • Vagera, J., & Havranek, P. (1985). In vitro induction of androgenesis in Capsicum annuum L. and its genetic aspects. Biologia Plantarum, 27(1), 10–21.

    Article  CAS  Google Scholar 

  • Vaidya, H., Rajani, M., Sudarsanam, V., Padh, H., & Goyal, R. (2009). Antihyperlipidaemic activity of swertiamarin, a secoiridoid glycoside in poloxamer-407-induced hyperlipidaemic rats. Journal of Natural Medicines, 63, 437–442.

    Article  PubMed  CAS  Google Scholar 

  • Weich, W. E., & Levall, M. W. (2003). Doubled haploid production of sugar beet (Beta vulgaris L.). In M. Maluszynski, K. J. Kasha, B. P. Forster, & I. Szarejko (Eds.), Doubled haploid production in crop plants: A manual (pp. 265–273). Dordrecht: Kluwer Academic Publishers.

    Google Scholar 

  • Wettasinghe, M., & Shahidi, F. (1999). Antioxidant and free radical-scavenging properties of ethanolic extracts of defatted borage (Borago officinalis L.) seeds. Food Chemistry, 67, 399–414.

    Article  CAS  Google Scholar 

  • Wolyn, D. J., & Nichols, B. (2003). Asparagus microspore and anther culture. In M. Maluszynski, K. J. Kasha, B. P. Forster, & I. Szarejko (Eds.), Doubled haploid production in crop plants: A manual (pp. 265–273). Dordrecht: Kluwer Academic Publishers.

    Chapter  Google Scholar 

  • Xu, L., Najeeb, U., Tang, G. X., Gu, H. H., Zhang, G. Q., He, Y., & Zhou, W. J. (2007). Haploid and doubled haploid technology. Advances in Botanical Research, 45, 181–216.

    Article  CAS  Google Scholar 

  • Yadav, J. S., Nanda, S. P., Reddy, P. T., & Rao, A. B. (2002). Efficient enantioselective reduction of ketones with Daucus carota root. The Journal of Organic Chemistry, 67(11), 3900–3903.

    Article  PubMed  CAS  Google Scholar 

  • Yamada, H., Kikuchi, S., Inui, T., Takahashi, H., & Kimura, K. (2014). Gentiolactone, a secoiridoid dilactone from Gentiana triflora, inhibits TNF-a, iNOS and Cox-2 mRNA expression and blocks NF-kB promoter activity in murine macrophages. PLoS One, 9(11), 113834.

    Article  CAS  Google Scholar 

  • Zhang, Y. X., Lespinasse, Y., & Chevreau, E. (1990). Induction of haploidy in fruit trees. Acta Horticulturae, (280), 293–304.

    Google Scholar 

  • Zhang, Z. L., Qiu, F. Z., Liu, Y. Z., Ma, K. J., Li, Z. Y., & Xu, S. Z. (2008). Chromosome elimination and in vivo haploid production induced by Stock 6-derived inducer line in maize (Zea mays L.). Plant Cell Reports, 27, 1851–1860.

    Article  PubMed  CAS  Google Scholar 

  • Zhao, F. C., Nilanthi, D., Yang, Y. S., & Wu, H. (2006). Anther culture and haploid plant regeneration in purple coneflower (Echinacea purpurea L.). Plant Cell, Tissue and Organ Culture, 86, 55–62.

    Article  Google Scholar 

  • Zubko, M. K., & Day, A. (2002). Differential regulation of genes transcribed by nucleus-encoded plastid RNA polymerase, and DNA amplification, within ribosome-deficient plastids in stable phenocopies of cereal albino mutants. Molecular Genetics and Genomics, 267, 27–37.

    Article  PubMed  CAS  Google Scholar 

  • Zur, I., Dubas, E., Golemiec, E., Szechynska-Hebda, M., Golebiowska, G., & Wedzony, M. (2009). Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (Tritico secale Wittm.). Plant Cell Reports, 28, 1279–1287.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. KET’s Scientific Research Centre, Mulund(East), Mumbai, 400081, India

    Sweta Sharma, Kshitij Vasant Satardekar & Siddhivinayak S. Barve

Authors
  1. Sweta Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kshitij Vasant Satardekar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Siddhivinayak S. Barve
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Biotechnology, Central University of South Bihar, Panchanpur, Gaya, Bihar, India

    Nitish Kumar

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Sharma, S., Satardekar, K.V., Barve, S.S. (2018). Genetic Improvement of Medicinal and Aromatic Plants Through Haploid and Double Haploid Development. In: Kumar, N. (eds) Biotechnological Approaches for Medicinal and Aromatic Plants. Springer, Singapore. https://doi.org/10.1007/978-981-13-0535-1_24

Download citation

Publish with us

Policies and ethics

Search

Navigation