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Super-Resolution Fluorescence Microscopy for Single Cell Imaging

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Single Cell Biomedicine

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1068))

Abstract

In the past two decades, super-resolution fluorescence microscopy has undergone a dynamic evolution. Following proof-of-concept studies with stimulated emission depletion (STED) microscopy, several new approaches such as structured illumination microscopy (SIM), photoactivation localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), have been developed for imaging of nanoscale structural details and fast cellular dynamics in biological research. In this chapter, after briefly explaining their principles, we will describe the recent application of these super-resolution techniques in single cell imaging. In addition, the extension of super-resolution microscopy to 3D, multicolor, live-cell imaging and multimodal imaging are also discussed, significantly improving the precision of single cell imaging. Combining with molecular biology, biochemistry and bio-computing algorithms, super-resolution fluorescence microscopy continues to expand its capabilities and provide comprehensive insights into the details of single cells.

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Acknowledgments

The authors gratefully appreciate the support from the National Natural Science Foundation of China (No.81472835 and 81670091) and the National Key Clinical Specialist Construction Programs of China (2013-544).

Conflicts of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Pharmacy, Henan Provincial People’s Hospital, and People’s Hospital of Zhengzhou University, Zhengzhou, China

    Han Feng

  2. Department of Nuclear Medicine, Henan Provincial People’s Hospital, and People’s Hospital of Zhengzhou University, Zhengzhou, China

    Xiaobo Wang & Yongju Gao

  3. Clinical Research Service Center, Henan Provincial People’s Hospital, and People’s Hospital of Zhengzhou University, Zhengzhou, China

    Xiaobo Wang, Zhiwei Xu & Xiaoju Zhang

  4. Department of Respiratory Medicine, Henan Provincial People’s Hospital, and People’s Hospital of Zhengzhou University, Zhengzhou, China

    Xiaoju Zhang

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  1. Han Feng
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  2. Xiaobo Wang
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  3. Zhiwei Xu
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  4. Xiaoju Zhang
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Correspondence to Xiaobo Wang .

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Editors and Affiliations

  1. Henan Provincial People’s Hospital, Zhengzhou, Henan, China

    Jianqin Gu

  2. Shanghai Institute of Clinical Bioinformatics, Zhongshan Hospital, Fudan University, Shanghai, China

    Xiangdong Wang

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Feng, H., Wang, X., Xu, Z., Zhang, X., Gao, Y. (2018). Super-Resolution Fluorescence Microscopy for Single Cell Imaging. In: Gu, J., Wang, X. (eds) Single Cell Biomedicine. Advances in Experimental Medicine and Biology, vol 1068. Springer, Singapore. https://doi.org/10.1007/978-981-13-0502-3_6

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