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In Vitro Morphogenesis of Some Himalayan Flora Using TDZ: A Potential Plant Growth Regulator

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Thidiazuron: From Urea Derivative to Plant Growth Regulator

Abstract

Thidiazuron or TDZ is a substituted phenylurea derivative, N-phenyl-N′-l, 2, 3-thidiazol-5-ylurea, extensively used in the in vitro morphogenesis of different plant species, including those found in Himalayas. The plants have ranged from the advanced monocotyledonous to dicotyledonous angiospermic plants to gymnosperms and even pteridophytes. While some of these plants are endemic to the Himalayas and are medicinally important, others have been introduced into the region for their immense commercial values. Optimal morphogenic responses obtained in these plants have varied with the concentration of TDZ used and its duration of exposure. These in turn have depended upon the explant and the media used, and also the plant species targeted. While in some cases TDZ alone evoked the desired responses, a combination of TDZ with other PGRs was required in other cases. The findings of these reports have been reviewed in the present article. Attempts have also been made to collate these findings into a ready reckoner for researchers working on in vitro morphogenesis of various plants inhabiting the Himalayas.

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Abbreviations

2, 4-D:

2, 4-Dichlorophenoxyacetic acid

B5:

Gamborg medium

BAP:

Benzylamino purine

DCR:

Douglas-fir cotyledon revised medium

Kn:

Kinetin

LS:

Linsmaier and Skoog medium

MRM:

Modified rhododendron medium

MS:

Murashige and Skoog medium

NAA:

Naphthalene acetic acid

nM:

Nanomole

PGR:

Plant growth regulator

PLBs:

Protocorm-like bodies

pM:

Picomole

TDZ:

Thidiazuron

WPM:

Woody plant medium

μM:

Micromole

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Acknowledgements

The authors thank Director, CSIR-IHBT, Palampur for providing the infrastructure. ND thanks the University Grants Commission, Govt. of India for providing Senior Research Fellowship. NG thanks the Department of Biotechnology, Govt. of India for providing her fellowship; BS thanks the Indian Council of Medical Research (ICMR), Govt. of India for providing Senior Research Fellowship. The CSIR-IHBT communication number for the present article is 4167.

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Authors and Affiliations

  1. Academy of Scientific and Innovative Research, New Delhi, India

    Nisha Dhiman & Amita Bhattacharya

  2. Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP, India

    Nisha Dhiman, Nitu Gautam, Bhuvnesh Sareen, Pooja Kumari, Sapna Rajouria & Amita Bhattacharya

Authors
  1. Nisha Dhiman
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  2. Nitu Gautam
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  3. Bhuvnesh Sareen
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  4. Pooja Kumari
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  5. Sapna Rajouria
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  6. Amita Bhattacharya
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Corresponding author

Correspondence to Amita Bhattacharya .

Editor information

Editors and Affiliations

  1. Department of Botany, Aligarh Muslim University, Aligarh, UP, India

    Naseem Ahmad

  2. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Mohammad Faisal

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Dhiman, N., Gautam, N., Sareen, B., Kumari, P., Rajouria, S., Bhattacharya, A. (2018). In Vitro Morphogenesis of Some Himalayan Flora Using TDZ: A Potential Plant Growth Regulator. In: Ahmad, N., Faisal, M. (eds) Thidiazuron: From Urea Derivative to Plant Growth Regulator. Springer, Singapore. https://doi.org/10.1007/978-981-10-8004-3_12

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