Abstract
This is the simplest cloning strategy, which uses two RE sites in the MCS of a vector. Below are the step by step descriptions of the cut and paste method (Fig. 3.1).
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The primer mentioned here has nothing to do with the RT reaction. Strictly speaking, primers bind to the cDNA synthesized from the RT reaction, but they are commonly called RT-PCR primers . To be accurate, they should be called cDNA primers
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Choi, SY., Ro, H., Yi, H. (2019). The First Step in Cloning. In: DNA Cloning: A Hands-on Approach. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1662-6_3
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DOI: https://doi.org/10.1007/978-94-024-1662-6_3
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