Skip to main content

Summary

By means of Immunoelectrophoresis we have observed the formation of antibodies and immunoelectrophoretic differences between spermatozoa and seminal plasma of bulls and the corresponding antibodies. We have found that bull seminal plasma forms with rabbit antiserum against seminal plasma a precipitation arc which passes through the β1 region and reaches the albumin region, further three arcs passing through the β1 region and reaching the α1 region and then one arc which is placed in the β1 region and reaches region α2 and yet another one which may be found in the β2 region. Ejaculated bull spermatozoa form with rabbit antiserum against ejaculated spermatozoa a continuous arc from the antigen reservoir up to the albumin region. This continuous arc, however, splits in the α1 and albumin regions. Another precipitation arc is formed in region β1. Ampullar spermatozoa of bulls form with rabbit antiserum against ampullar spermatozoa similar reactions. Spermatozoa from the tail of the epididymis of bulls form with rabbit antiserum against spermatozoa from the tail of the epididymis a precipitation arc which is continuous up to the α1 region only. Another arc is formed in region β1. Cross reactions between individual antigens were also investigated and their reciprocal immunological relationship was detected.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Aronsson, T. and Grønwall, A., 1957. Cited by Michalec et al., 1959. Elektroforeza na papíře a jiných nosičich, 1–135, NČSAV, Praha.

    Google Scholar 

  • Brummerstedt-Hansen, E., 1961. Immunoelectrophoretic investigations on the blood serum of adult pigs, Acta Vet. Scand., 2, 254–262.

    Google Scholar 

  • Edwards, R. G., 1960. Complement-fixing activity of normal rabbit serum with rabbit spermatozoa and seminal plasma, J. Repr. Fert., 1, 268–282.

    Article  Google Scholar 

  • Grabar, P., 1958. The use of immunochemical methods in studies on proteins, Advances in Protein Chemistry, 12, 1–33, Academic Press Inc.. New York.

    Google Scholar 

  • Henle, W., 1938. The specificity of some mammalian spermatozoa, J. Imm., 34, 325–336.

    Google Scholar 

  • Landsteiner, K., 1899. Cited by Weil, A. J., 1961. Antigens of the adnexal glands of the male genital tract, Fert. Ster., 12, 538–543.

    Google Scholar 

  • Maruta, H. and Moyer, D. L., 1967. Immunologic studies of the antigens of guinea pig semen, Fert. Ster., 18, 649–658.

    CAS  Google Scholar 

  • Matousek, J., 1961. Antigenic factor J in some body fluids of cattle, Folia Biol. (Praha), 7, 252–257.

    CAS  Google Scholar 

  • Matousek, J., 1962. The study of the antigenic characteristics of sperm from bulls, The 8th Animal Blood Group Conference in Europe, Ljubljana, Yugoslavia.

    Google Scholar 

  • Matousek, J., 1964. Antigenic characteristics of spermatozoa from bulls, rams and boars. III. Absorptions analyses, precipitins and fructolysis in relation to the antigenicity of bull spermatozoa, J. Repr. Fert., 8, 13–21.

    Article  CAS  Google Scholar 

  • Mudd S. and Mudd, E. B. H., 1929. The specificity of mammalian spermatozoa with special reference to electrophoresis as a means of serological differentiations, J. Imm., 17, 39–51.

    Google Scholar 

  • Pernot, E., 1956. Recherches sur les constituants antigéniques des spermatozoïdes de cobayes, Bull. Soc. Chim. Biol., 38, 1041–1054.

    CAS  PubMed  Google Scholar 

  • Pernot, E. and Szumowski, P., 1958. Étude electrophorétique et immunoélectrophorétique des proteins du plasma séminal de taureau, Bull. Soc. Chim. Biol., 40, 1423–1434.

    CAS  PubMed  Google Scholar 

  • Ross, V., 1946. Precipitin reactions of human seminal plasma, J. Imm., 52, 87–96.

    CAS  Google Scholar 

  • Scheidegger, J., 1955. Une micro-méthode de l’immunoélectrophorése, Int. Arch. Allergy Appl. Immun., 7, 103–109.

    Article  CAS  Google Scholar 

  • Schmid, D. O., Connealy, P. N. and Stone, W. H., 1964. Blood group antigens on bull spermatozoa, Meeting of the Midwestern Section A.S.A.S., November 27–28, Chicago, Illinois.

    Google Scholar 

  • Weil, A. J., Kotsevalov, O. and Wilson, L., 1956. Antigens of human seminal plasma, Proc. Soc. Exp. Biol. Med., 92, 606–610.

    Article  PubMed  Google Scholar 

  • Weil, A. J. and Finkler, A. E., 1958. Antigens of rabbit semen, Proc. Soc. Exp. Biol. Med., 98, 794–797.

    Article  CAS  PubMed  Google Scholar 

  • Weil, A. J., 1960. Immunological differentiation of epididymal and seminal spermatozoa of the rabbit, Science, 131, 1040–1041.

    Article  CAS  PubMed  Google Scholar 

  • Weil, A. J. and Rodenburg, J. M., 1960. Immunological differentiations of hunam testicular (spermatocele) and seminal spermatozoa, Proc. Soc. Exp. Biol. Med., 105, 43–45.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 1970 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Veselský, L. (1970). Immunoelectrophoretical Study on Seminal Plasma and Spermatozoa from Bulls. In: XIth European Conference on Animal Blood Groups and Biochemical Polymorphism. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-5226-8_21

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-5226-8_21

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-017-5228-2

  • Online ISBN: 978-94-017-5226-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics