Investigation on the Production of Immune Antisera in Cattle: Three New Reagents Anti-I₀ -G₀, -R₀

Summary

In an effort to produce reagents for red blood cell antigens in cattle, three reagents anti-I₂, anti-G₂, and anti-R₂ were produced which showed interesting patterns of reaction. These reagents, when diluted, gave much weaker reactions with red blood cells, possessing the I₁, G₁ and R₁ antigens.

After absorption with any of the weakly reacting red cells, the reagents still had antibodies against the I₂, G₂, R₂ antigens. We called these reagents anti-I₀, -G₀, -R₀.

Using the reagent anti-I₀, 5600 cattle were tested. They represent four breeds: Black and White, Polish Red, Danish Red and Simental cattle.

It was observed that the reagent anti-I₀ gave reactions in all cases (872) where the antigen I₂ occurred without I₁.

There were 490 individuals with I₁ antigen, but only 26 of them gave reaction withanti-I₀. When the genotypes were established for this group of 26 individuals it appeared that each of them had in one phenogroup the blood factor I₁ and in the other the I₀ antigen.

The results demonstrate that reagent anti-I₀ identifies the product of the gene which we propose to call B¹⁰, since antigen I₂ is produced by both genes.