Abstract
Mononuclear phagocytic cells are implicated in many chronic inflammatory diseases1. In rheumatoid arthritis these cells, stimulated by inflammatory products, engulf immune complexes accumulating in the diseased joint, accompanied by a selective secretion of lysosomal acid hydrolases2. These have the capacity to activate complement and kinin pathways as well as inducing the destruction of the proteoglycan matrix of the joint3. Inhibition of the release of these hydrolases by lysosomal membrane stabilizing drugs may be an important means of reducing joint destruction in chronic arthritis3. Many anti-inflammatory drugs, e.g. hydrocortisone, dexamethasone, phenylbutazone and flufenamic acid, have been reported to stabilize lysosomal membranes4–6. With some of these compounds a correlation has been demonstrated between lysosomal stabilization effects in vitro and their antiinflammatory activities in vivo in animal models4.
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References
Loeui, G. (1975). Inflammatory arthritis: immunology and inflammation at the cellular level. In Holt, P. J. L. (ed.) Current Topics in Connective Tissue Diseases, p. 48. (London: Churchill-Livingstone)
Davies, P. and Allison, A. C. (1976). The macrophage as a secretory cell in chronic inflammation. Agents Actions, 6, 60
Weissman, G. (1971). Lysosomes and the mediation of tissue injury in arthritis. In Muller, W., Harwerth, H. G. and Fehr, K. (eds.) Rheumatoid Arthritis, p. 141. (London: Academic Press)
Tanaka, K. and Iizuka, Y. (1968). Suppression of enzyme release from isolated rat liver lysosomes by non-steroidal anti-inflammatory drugs. Biochem. Pharmadol., 17, 2023
Ringrose, P. S., Parr, M. A. and McLaren, M. (1975). Effects of anti-inflammatory and other compounds on the release of lysosomal enzymes from macrophages. Biochem. Pharmacol, 24, 607
Smith, R. J. (1977). Phagocytic release of lysosomal enzymes from guinea-pig neutrophils-regulation by corticosteroids, autonomic neurohormones and cyclic nucleotides. Biochem. Pharmacol., 26, 2001
Burkhardt, D., Stephens, R. S., Ghosh, P. and Taylor, T. K.F. (1978). Inhibition of synovial fluid lysosomal glycosidases by anti-arthritic gold preparations. Agents Actions, 8, 251
Barrett, A. J. (1972). Lysosomal enzymes, In Dingle, J. T. (ed.) Lysosomes, a Laboratory Handbook. (Amsterdam: North-Holland Publishing)
Sigma Technical Bulletin No. 500. (1975). The quantitative colorimetric determination of lactate dehydrogenase. Sigma Chemical Co., St. Louis
Miller G. L. (1959). Protein determination for large numbers of samples. Anal. Chem., 31, 964
Picton, W., Devitt H. and Forgie M. A. (1976). Practical applications of the Quantimet 720, an image analysing computer in the field of investigative dermatology. Br. J. Dermatol., 95, 341
Walters, M. N.-I. and Papadimitriou, J. M. (1978). Phagocytosis; a review. CRC Crit. Rev. Toxicol, 5, 377
Smith, R. J. (1979). The guinea-pig neutrophil calcium-dependent lysosomal enzyme secretory process. Inhibition by non-steroidal anti-inflammatory agents. Biochem. Pharmacol., 28, 2739
Dean, R. T., Hylton, W. and Allison, A. S. (1979). Lysosomal enzyme secretion by macrophages during intracellular storage of particles. Biochem. Biophys. Acta, 584, 57
Walz, D. T., Di Martino, M. J., Chakrin, L. W., Sutton, B. M. and Misher, A. (1976). Anti-arthritic properties and unique pharmacologic profile of a potential chrysotherapeutic. Agent, S.K.&F. D-39162. J. Pharmacol. Exp. Ther., 197, 142
Finklestein, A.E., Walz, D.T., Batista, V., Mizraji, F., Roisman, F., and Misher, A. (1976). Auranofin. A new oral gold compound for treatment of rheumatoid arthritis. Ann. Rheum. Dis., 35, 251
Berglöf, F-E., Berglöf, K. and Walz, D.T. (1978). Auranofin; an oral chrysotherapeutic agent for the treatment of rheumatoid arthritis. J. Rheumatol, 5, 68
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White, D.D., Fox, P.K., Livingston, P. (1980). An in vitro mouse macrophage model for use in assessing the effects of anti-inflammatory drugs. In: Willoughby, D.A., Giroud, J.P. (eds) Inflammation: Mechanisms and Treatment. Inflammation: Mechanisms and Treatment, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-9423-8_52
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DOI: https://doi.org/10.1007/978-94-010-9423-8_52
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-9425-2
Online ISBN: 978-94-010-9423-8
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