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Optical Studies on T4 Gene Product 32 Protein DNA Interaction

  • Conference paper
Excited States in Organic Chemistry and Biochemistry

Part of the book series: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ((JSQC,volume 10))

Abstract

Bacteriophage T4 gene 32 coded protein (gp32) was isolated by Alberts and coworkers (1,2,3). It binds tightly, cooperatively and preferentially to single-stranded DNA. It facilitates both the denaturation and the renaturation of DNA. Genetic studies have demonstrated that this protein is essential for genetic recombination (4), DNA replication (5), repair of radiation damaged DNA (6), and protection of replicating T4 chromosome from degradative activities (7,8). In vitro, gp32 enhances the rate at which T4 DNA polymerase utilizes single-stranded DNA temperates by 5 to 10-fold (9). Gp32 is also one of the essential components of the reconstructed DNA replicating apparatus (11).

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References

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Author information

Authors and Affiliations

  1. Space Sciences Laboratory Berkeley, University of California, California, USA

    Marcos F. Maestre, Jan Greve & Junko Hosoda

Authors
  1. Marcos F. Maestre
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  2. Jan Greve
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  3. Junko Hosoda
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Editor information

Editors and Affiliations

  1. Institut de Biologie Physico-Chimique (Fondation Edmond de Rothschild), Université Pierre et Marie Curie (Paris VI), 13 rue P. et M. Curie, Paris 5e, France

    Bernard Pullman

  2. Hadassah Medical School, The Hebrew University, Jerusalem, Israel

    Natan Goldblum

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© 1977 Springer Science+Business Media Dordrecht

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Maestre, M.F., Greve, J., Hosoda, J. (1977). Optical Studies on T4 Gene Product 32 Protein DNA Interaction. In: Pullman, B., Goldblum, N. (eds) Excited States in Organic Chemistry and Biochemistry. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 10. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-1273-7_9

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  • DOI: https://doi.org/10.1007/978-94-010-1273-7_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-1275-1

  • Online ISBN: 978-94-010-1273-7

  • eBook Packages: Springer Book Archive

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