Abstract
A variety of enzyme-linked immunosorbent assay (ELISA) systems are being applied in the detection and quantification of both antibodies and antigens in bacterial, viral and parasitic infections. To date, the use of ELISA in research on neonatal diarrhoea has been described for the detection of rotavirus, bovine coronavirus and enteropathogenic Escherichia coli infections.
Some features of the double antibody sandwich ELISA system are discussed with particular reference to those elements most important in determining the specificity and sensitivity of the test.
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Keywords
- Horse Radish Peroxidase
- Double Antibody Sandwich
- Alkaline Phosphatase Substrate
- Candida Antigen
- Bovine Coronavirus
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© 1981 ECSC, EEC, EAEC, Brussels-Luxembourg
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Ellens, D.J. (1981). Diagnosis by Enzyme-Linked Immunosorbent Assay. In: de Leeuw, P.W., Guinée, P.A.M. (eds) Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea. Current Topics in Veterinary Medicine and Animal Science, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-8328-1_3
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DOI: https://doi.org/10.1007/978-94-009-8328-1_3
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