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Naunyn Schmiedebergs Archiv für Pharmakologie pp 1512–1536Cite as

Freisetzung von Serotonin und Histamin aus Thrombocyten durch aliphatische und aromatische Amine

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Summary

The Release of Serotonin and Histamine from Thrombocytes by Aliphatic and Aromatic Amines.

In studies with washed platelets and with platelet-rich plasma of the rabbit the following results were obtained:

  1. 1

    Incubation of thrombocytes with aliphatic amines having different lengths of the carbon chain (C6–C10) produced a dose-dependent decrease of the serotonin and histamine content of the cells. Amines with a long carbon chain (e.g. C10, C9) were 10–20 times more effective than those having a short chain length (e.g. C6, C7). While C10 and lysolecithin lead to an approximative equal decrease in serotonin and histamine content of the cells, C6 mainly induced a liberation of serotonin. Incubation of the cells with C10 or lysolecithin induced a very rapid amine liberation, while C6 and C7 had a slow amine releasing action upon the platelets.

  2. 2

    Aromatic amines (e.g. phenylethylamine, tyramine) showed a selective and dose-dependent liberation of serotonin from the thrombocytes; only very high concentrations (e.g. 1.2 mg/ml) also reduced the histamine level. Introduction of a second phenolic hydroxyl group in the ring (catechol derivatives) or of an alcoholic hydroxyl group into the side chain diminished the serotonin liberating effect of the amines. Methylation of the amine group or of the alpha-carbon atom of the side chain did not influence their efficacy. — Cocaine or desmethylimipramine had no inhibitory effect upon the serotonin liberation induced by tyramine.

  3. 3

    It is assumed, that the histamine liberation induced by aliphatic amines is the result of a cytolysis,—very similar to the action of lysolecithin; this histamine liberating action corresponds to the hemolytic and surface-active properties of these substances.—In contrast, the selective and preferential liberation of serotonin by aromatic amines and by aliphatic amines with a short carbon chain is very likely due to a displacement of serotonin from the storage sites in the intact cells.

Herrn Professor Dr. G. Kuschinskyzum 65. Geburtstag gewidmet.

Über einen Teil der Ergebnisse haben wir auf der 8. Frühjahrstagung der Deutschen Pharmakologischen Gesellschaft (Mayet al., 1967 a) sowie in einer kurzen Mitteilung (Mayet al., 1967 c) berichtet.

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Authors and Affiliations

  1. Institut für Pharmakologie, Medizinischen Hochschule, 3000, Hannover-Buchholz, Roderbruchstraße 101, Deutschland

    B. May, I. Menkens & Prof. Dr. med. Erik Westermann

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  1. B. May
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  2. I. Menkens
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  3. Prof. Dr. med. Erik Westermann
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Editors and Affiliations

  1. Gießen, Deutschland

    E. Habermann

  2. Berlin, Deutschland

    H. Herken

  3. Frankfurt/M., Deutschland

    P. Holtz

  4. Graz, Österreich

    F. Lembeck

  5. Würzburg, Deutschland

    U. Trendelenburg

  6. Ulm, Deutschland

    L. Heilmeyer

  7. Göttingen, Deutschland

    L. Lendle

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May, B., Menkens, I., Westermann, E. (1969). Freisetzung von Serotonin und Histamin aus Thrombocyten durch aliphatische und aromatische Amine. In: Habermann, E., et al. Naunyn Schmiedebergs Archiv für Pharmakologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-39718-3_309

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  • DOI: https://doi.org/10.1007/978-3-662-39718-3_309

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