Summary
The metabolism of 4-androstene-3, 17-dione has been studied in rat liver microsomes. Treatment of the animals with repeated doses of phenobarbital in vivo caused an enhanced rate of formation of 5α-androstane-3, 17-dione and of polar metabolites from this steroid. On the other hand, no significant increase was found in the 5β-reductase activity present in the soluble cytoplasm after phenobarbital administration to the rats. Carbon monoxide and oxidized cytochrome c abolished the formation of polar metabolites from 4-androstene-3, 17-dione but did not inhibit the 5α-reductase activity. The present findings indicate that the hydroxy-lation and 5α-reduction reactions, although both stimulated by phenobarbital treatment of the animals, do not involve common enzyme components.
Supported by grants from the Swedisch Cancer Society, Swedish Medical Research Council (Project no. 13X-2525) and from Caroline Andriette Nobel’s foundations for experimental medical research.
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von Bahr, C., Lisboa, B.P., Orrenius, S. (1969). Influence of Phenobarbital Pretreatment on the in Vitro Ring A Reduction of Δ4-3-Oxo-Steroids in Rat Liver Microsomes. In: Habermann, E., et al. Naunyn Schmiedebergs Archiv für Pharmakologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-39718-3_302
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