Skip to main content
SpringerLink
Log in
Book cover

Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz pp 776–782Cite as

Zur Seroprävalenz von Herpes-simplex-Virus Typ 2 (HSV-2) in Thüringen

Seroprevalence of Herpes simplex virus type 2 infections in blood donors and clinic patients in Thuringia, Germany

  • Chapter

  • 6 Accesses

Zusammenfassung

Herpes-simplex-Viren (HSV-1, HSV-2) sind die häufigste infektiöse Ursache von genitalen Ulzerationen.Trotz einer Zunahme primärer genitaler HSV-1-Infektionen ist HSV-2 der dominante Virustyp bei rezidivierenden Erkrankungen. Rückschlüsse auf die Häufigkeit des genitalen Herpes können daher aus der Prävalenz von HSV-2-spezifischen Antikörpern in der Bevölkerung gezogen werden. Diese Studie wurde an einem Panel von Serumproben durchgeführt, das 1996 und 1997 anonym von 1979 Blutspendern und 3079 Klinikpatienten gesammelt worden war. Die Patienten hatten keine bekannten Risikofaktoren bezüglich HSV-Infektionen wie sexuell übertragbare Krankheiten, andere Infektionskrankheiten oder eine immunsuppressive Therapie. Die Serumproben wurden mit typenspezifischen ELISA-Testen, die auf den gereinigten nativen Glykoproteinen gG1 bzw.gG2 basieren sowie einem Immunoblot mit typenspezifischen rekombinanten Glykoproteinen untersucht. Die Abklärung fraglicher Reaktionsausfälle erfolgte mit einem In-Haus-Westernblot.

Die HSV-1-Seroprävalenz korrelierte eng mit dem Alter und war bei Personen ≥40 Jahre mit ca. 90% am höchsten. Im Gesamtkollektiv hatten 12,8% (95% CI 11,9-13,8%) der Probanden Antikörper gegen HSV-2. Die HSV-2-Seroprävalenz war bei Frauen (15%; 95% CI 13,7-16,4%) signifikant höher als bei Männern (10,5%; 95% CI 9,3-11,8%), was einem altersadjustierten Odds Ratio für Patienten von 1,5 (95%-CI 1,19-1,90) und für Blutspender von 1,67 (95%-CI 1,29-2,15) entspricht.

Summary

Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) are the most frequent infections cause of genital ulcerations. Although genital infections by HSV-1 are increasing HSV-2 is dominant in recurrent infections. This study was conducted on a panel of sera collected anonymously in 1996 and 1997 from blood donors (1979 samples) and hospital patients (3079 samples) without risk factors for herpesvirus infections such as STD, other infectious diseases and immunosuppression. Serum samples were tested with ELISAs using monoclonal antibody-selected native gG1 and gG2 as antigens and an immunoblot using type-specific recombinant glycoproteins. Equivocal results were confirmed with an in-house Western blot. HSV-1 prevalence correlated strongly with age and was highest (about 90%) among subjects 40 years of age or older. In the total sample, the HSV-2 seroprevalence was 12.8% (95% CI 11.9-13.8%). The HSV-2 seroprevalence was significantly higher among women (15%; 95% CI 13.7-16.4%) than among men (10.5%; 95% CI 9.3-11.8%), yielding a female: male age adjusted odds ratio of 1.5 (95%-CI 1.19-1.90%) for hospital patients and of 1.67 (95%-CI 1.29–2.15%) for blood donors.

Diese Studie wurde aus Mitteln des Bundesministeriums für Gesundheit vom Robert Koch-Institut (RKI) unter dem Förderkennzeichen 1368-319 gefördert.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   199.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Schneweis KE (1962) Serologische Untersuchungen zur Typendifferenzierung des Herpesvirus hominis. Z Immuno Forsch 124:24–28.

    CAS  Google Scholar 

  2. Woolley PD, Kudesia G (1990) Incidence of herpes simplex virus type-1 and type-2 from patients with primary (first-attack) genital herpes in Sheffield. Int J STD AIDS 1:184–186.

    CAS  PubMed  Google Scholar 

  3. Edwards S, White C (1994) Genital herpes simplex virus type 1 in women. Genitourin Med 70:426.

    CAS  PubMed Central  PubMed  Google Scholar 

  4. Reeves WC, Corey L, Adams HG, Vontver LA, Holmes KK (1981) Risk of recurrence after first episodes of genital herpes. Relation to HSV type and antibody response. N Engl J Med 305:315–319.

    Article  CAS  PubMed  Google Scholar 

  5. Breinig MK, Kingsley LA, Armstrong JA, Freeman DJ, Ho M (1990) Epidemiology of genital herpes in Pittsburgh: serologic, sexual, and racial correlates of apparent and inapparent herpes simplex infections. J Infect Dis 162:299–305.

    Article  CAS  PubMed  Google Scholar 

  6. Mertz GJ, Benedetti J, Ashley R, Selke SA, Corey L (1992) Risk factors for the sexual transmission of genital herpes. Ann Intern Med 116:197–202.

    Article  CAS  PubMed  Google Scholar 

  7. Ashley R (1993) Laboratory techniques in the diagnosis of herpes simplex infection. Genitourin Med 69:174–183.

    CAS  PubMed Central  PubMed  Google Scholar 

  8. Lee FK, Coleman RM, Pereira L, Bailey PD, Tatsuno M, Nahmias AJ (1985) Detection of herpes simplex virus type 2-specific antibody with glycoprotein G.J Clin Microbiol 224:641–644.

    Google Scholar 

  9. Lee FK, Pereira L, Griffin C, Reid E, Nahmias A (1986) A novel glycoprotein for detection of herpes simplex virus type 1-specific antibodies. J Virol Methods 14:111–118.

    Article  CAS  PubMed  Google Scholar 

  10. Bergström T, Trybala E (1996) Antigenic differences between HSV-1 and HSV-2 glycoproteins and their importance for type-specific serology. Intervirology 39:176–184.

    PubMed  Google Scholar 

  11. Ashley R (1998) Type-specific antibodies to HSV1 — and-2: Review of methodology. Herpes 5:33–38.

    Google Scholar 

  12. Ashley RL, Wu L, Pickering JW, Tu MC, Schnorenberg L (1998) Premarket evaluation of a commercial glycoprotein G-based enzyme immunoassay for herpes simplex virus type-specific antibodies. J Clin Microbiol 36: 294–295.

    CAS  PubMed Central  PubMed  Google Scholar 

  13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685.

    Article  CAS  PubMed  Google Scholar 

  14. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354.

    Article  CAS  PubMed  Google Scholar 

  15. Sachs L (1992) Angewandte Statistik: Anwendung statistischer Methoden. 7. Auflage. Springer, Berlin Heidelberg New York.

    Google Scholar 

  16. Flemming DT, McQuillan GM, Johnson RE, Nahmias AJ, Aral SO, Lee FK, St Louis ME (1997) Herpes simplex virus type 2 in the United States, 1976 to 1994. N Engl J Med 337:1105–1111.

    Article  Google Scholar 

  17. Cowan FM, Johnson AM, Ashley R, Corey L, Mindel A (1994) Antibody to herpes simplex virus type 2 as a serological marker of sexual lifestyle in populations. Brit Med J 309:1325–1329.

    Article  CAS  PubMed  Google Scholar 

  18. Kjaer SK, de Viliers EM, Caglayan H, Svare E, Haugaard BJ, Engholm G, Christensen RB, Möller KA, Poll P, Jensen H et al (1993) Human papillomavirus, herpes simplex virus and other potential factors for cervical cancer in a high-risk area (Greenland) and a lowrisk area (Denmark)-a second look. Br J Cancer 67:830–837.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Bahrdt B, Rabenau H, Weber B, Eibner J, Doerr HW (1991) Prevalence of herpes-simplexvirus type 2 specific antibodies in patients with different risks of infection. Z Hautkr 67:56–58.

    Google Scholar 

  20. Enders G, Risse B, Zauke M, Bolley I, Knotek F (1998) Seroprevalence study of herpes simplex virus type 2 among pregnant women in Germany using a type-specific enzyme immunoassay. Eur J Clin Microbiol Infect Dis 17:870–872.

    Article  CAS  PubMed  Google Scholar 

  21. Nahmias AJ, Lee FK, Beckman-Nahmias S (1990) Sero-epidemiological and-sociological patterns of herpes simplex virus infection in the world. Scand J Infect Dis Suppl 69:19–36.

    CAS  PubMed  Google Scholar 

  22. Gibson JJ, Hornung CA, Alexander GR, Lee FL, Potts WA, Nahmias AJ (1990) A cross-sectional study of herpes simplex virus types 1 and 2 in college students: occurrence ad determinations of infection. J Infect Dis 162:306–312.

    Article  CAS  PubMed  Google Scholar 

  23. Bassett I, Donovan B, Bodsworth NJ, Field PR, Ho DW, Jeansson S, Cunningham AL (1994) Herpes simplex virus type 2 infection of heterosexual men attending a sexual health centre. Med J Aust 160:697–700.

    CAS  PubMed  Google Scholar 

  24. Siegel D, Golden E, Washington AE, Morse SA, Fullilove MT, Catania JA, Marin B, Hulley SB (1992) Prevalence and correlates of herpes simplex infections. The population-based AIDS in Multiethnic neighborhoods study. JAMA 268:1702–1708.

    Article  CAS  PubMed  Google Scholar 

  25. Johnson RE, Nahmias AJ, Magder LS, Lee FK, Brooks CA, Snowden CB (1989) A seroepidemiologic survey of the prevalence of herpes simplex virus type 2 infection in the United States. N Engl J Med 321:7–12.

    Article  CAS  PubMed  Google Scholar 

  26. Corey L (1994) The current trend in genital herpes. Progress in prevention. Sex Transm Dis 21 [2 Suppl]: 38–44.

    Google Scholar 

  27. van de Laar MJ, Jermorshuizen F, Slomka MJ, van Doornum GJ, Ossewaarde JM, Brown DW, Coutinho RA, van den Hoek JA (1998) Prevalence and correlates of herpes simplex virus type 2 infection: evaluation of behavioural risk factors. Int J Epidemiol 27:127–134.

    Article  PubMed  Google Scholar 

  28. Siegel D, Golden E, Washington AE, Morse SA, Fullilove MT, Catania JA, Marin B, Hulley SB (1992) Prevalence and correlates of herpes simplex infections. The population-based AIDS in multiethnic Neighborhoods Study. JAMA 268:1702–1708.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Medizinische Statistik, Informatik und Dokumentation, Friedrich-Schiller-Universität, Jena, Deutschland

    I. Färber, U. Eichhorn, B. Helbig & A. Sauerbrei

  2. Institut für Antivirale Chemotherapie, Friedrich-Schiller-Universität Jena, Nordhäuser Straße 78, D-99089, Erfurt, Deutschland

    Prof. Dr. Peter Wutzier & A. Brandstädt

Authors
  1. Prof. Dr. Peter Wutzier
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Färber
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. U. Eichhorn
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. Helbig
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Sauerbrei
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Brandstädt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1999 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Wutzier, P., Färber, I., Eichhorn, U., Helbig, B., Sauerbrei, A., Brandstädt, A. (1999). Zur Seroprävalenz von Herpes-simplex-Virus Typ 2 (HSV-2) in Thüringen. In: Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-38283-7_141

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-38283-7_141

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-37515-0

  • Online ISBN: 978-3-662-38283-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation