Abstract
The prfB gene encodes pep tide-chain-release factor 2 which catalyzes translation termination at nonsense codons UGA and UAA in bacteria. Mutations in prfB cause misreading of UGA, i.e., increased frameshift or suppression. These translational errors were due to inefficient translation termination at UGA. As one extreme case, an opal (UGA) mutation in prfB is autogenously suppressed. The prfB gene is in the same operon with herC, a gene defined by a suppressor mutation of a replication-defective ColEl plasmid. The herC gene coincides with lysS, a gene encoding a major species of lysyl-tRNA synthetase. Thus, the genetic organization of prfB and lysS in the same operon may suggest structural, functional and evolutional relevance of these gene products.
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© 1990 Springer-Verlag Berlin Heidelberg
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Nakamura, Y., Kawakami, K., Mikuni, O. (1990). Alternative Translation and Functional Diversity of Release Factor 2 and Lysyl-tRNA Synthetase. In: McCarthy, J.E.G., Tuite, M.F. (eds) Post-Transcriptional Control of Gene Expression. NATO ASI Series, vol 49. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75139-4_42
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DOI: https://doi.org/10.1007/978-3-642-75139-4_42
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