Skip to main content
SpringerLink
Log in

Ektacytometric Analysis of Factors Regulating Red Cell Deformability

  • Conference paper
Automation in Hematology

  • 56 Accesses

Abstract

Photometric analysis of laser diffraction patterns has been used to obtain quantitative measurements of deformability of specifically modified normal red cells. Variation of deformability with suspending medium osmolality and with applied shear stress was used to distinguish between changes in internal viscosity, surface area-to-volume ratio, and viscoelastic properties of the membrane in their influence on whole cell deformability.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. ALLARD, C., MOHANDAS, N., BESSIS, M.: Red cell deformability changes in hemolytic anemias estimated by diffractometric methods (ektacytometry). Blood Cells 3, 209, 1977

    Google Scholar 

  2. BESSIS, M., MOHANDAS, N.: A diffractometric method for the measurement of cellular deformability. Blood Cells 1, 307, 1975

    Google Scholar 

  3. BESSIS, M., MOHANDAS, N., FEO, C.: Automated ektacytometry: A new method of measuring red cell indices and red cell quality. Blood Cells 6, 315–327, 1980

    CAS  PubMed  Google Scholar 

  4. BESSIS, M., MOHANDAS, N.: Deformability of normal, shape-altered and pathological red cells. Blood Cells 1, 315, 1975

    Google Scholar 

  5. CLARK, M.R., MOHANDAS, N., CAGGIANO, V., SHOHET, S.B.: Effect of abnormal cation transport on deformability of desiccytes. J. Supramol. Struct. 8, 521, 1978

    Article  CAS  PubMed  Google Scholar 

  6. CLARK, M.R., MOHANDAS, N., SHOHET, S.B.: Deformability of oxygenated irreversibly sickled cells. J. Clin. Invest. 65, 189, 1980

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. LA CELLE, P.L.: Alterations of membrane deformability in hemolytic anemias. Semin. Hematol. 7, 355, 1970

    Google Scholar 

  8. LEBLOND, P.F., COULOMBE, L.: The measurement of erythrocyte deformability using micropore membranes. A sensitive technique with clinical applications. J. Lab. Clin. Med. 94, 133, 1979

    CAS  PubMed  Google Scholar 

  9. MOHANDAS, N., BOISFLEURY DE A.: Antibody-induced spherocytic anemia. I. Changes in red cell deformability. Blood Cells 3, 187, 1977

    Google Scholar 

  10. MOHANDAS, N., GREENQUIST, A.C., SHOHET, S.B.: Bilayer balance and regulation of red cell shape changes. J. Supramol. Struct. 9, 453, 1978

    Article  CAS  PubMed  Google Scholar 

  11. MOHANDAS, N., PHILLIPS, W.M., BESSIS, M.: Red blood cell deformability and hemolytic anemias. Semin. Hematol. 16, 95, 1979

    CAS  PubMed  Google Scholar 

  12. MURPHY, J.R.: Hemoglobin cc disease. Rheological properties of erythrocytes and abnormalities in cell water. J. Clin. Invest. 47, 1483, 1968

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. RAKOW, A.L., HOCHMUTH, R.M.: Effect of heat treatment on the elasticity of human erythrocyte membrane. Biophys. J. 15, 1095, 1975

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  14. USAMI, S., CHIEN, S., BERTLES, J.F.: Deformability of sickle cells as studied by microsieving. J. Lab. Clin. Med. 86, 274, 1975

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cancer Research Institute, University of California, 94143, San Francisco, CA, USA

    Narla Mohandas, Margaret R. Clark, Mark S. Jacobs & Stephen B. Shohet

  2. Institut de Pathologie Cellulaire, Hôpital de Bicêtre, F-94270, Le Kremlin-Bicêtre, France

    Narla Mohandas

  3. Technicon Instruments Corp., 511 Benedict Avenue, 10591, Tarrytown, NY, USA

    Warren Groner

Authors
  1. Narla Mohandas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Margaret R. Clark
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mark S. Jacobs
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Warren Groner
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Stephen B. Shohet
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of North Carolina, School of Medicine, 27514, Chapel Hill, NC, USA

    Dennis W. Ross

  2. University of California, Donner Laboratory, 94720, Berkeley, CA, USA

    George Brecher

  3. Institut de Pathologie Cellulaire, Hôpital de Bicêtre, 94270, Bicêtre, France

    Marcel Bessis

Rights and permissions

Reprints and permissions

Copyright information

© 1981 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Mohandas, N., Clark, M.R., Jacobs, M.S., Groner, W., Shohet, S.B. (1981). Ektacytometric Analysis of Factors Regulating Red Cell Deformability. In: Ross, D.W., Brecher, G., Bessis, M. (eds) Automation in Hematology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67756-4_14

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-67756-4_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-10225-0

  • Online ISBN: 978-3-642-67756-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation