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Waveguide Evanescent Field Fluorescence and Scattering Microscopy: The Status Quo

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Optics, Photonics and Laser Technology

Part of the book series: Springer Series in Optical Sciences ((SSOS,volume 218))

Abstract

In the last few years Waveguide Evanescent Field Fluorescence (WEFF) and Scattering (WEFS) microscopy were developed which are alternatives to TIR and TIRF microscopy . Both technologies implement a slab waveguide-microscopy chip with a coupling grating. The technologies are described and compared to TIR and TIRF microscopy. The advantages of the waveguide method are clearly addressed. A brief history of the technology’s development and similar activities in the field are discussed. Application examples from both WEF microscopies follow: static distance mapping with a multimode waveguide, dynamic solubilisation studies of cell plasma membranes and the kinetic response of osteoblasts to trypsin (WEFF); bacteria sterilization as well as cell adhesion and granularity studies (WEFS). The combination of both methods is discussed and found not suitable. In order to mass fabricate the necessary waveguide chips with the grating an all-polymer-waveguide chip was developed. This should allow to bring the new microscopy methods to the interested scientific community.

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Acknowledgements

Many co-workers, students, PDFs and colleagues are thanked for their contribution in the past, the present and the future for developing and applying WEFF and WEFS microscopy: Frank Thoma, Uwe Langbein, John J. Armitage, Huge Trembley, Michael Nietsche, Abdollah Hassanzadeh, Elisabeth Pruski, Jeffrey S. Dixon, Stephen Sims, Rebbeca Stuchburry, Sabiha Hacibekiroglu, Daniel Imruck, Christopher Halfpap, Michael Morawitz, Qamrun Nahar, Darryl K. Knight, Susanne Armstrong, Jeremia Shuster, Frederik Fleisser, Mihaela Stefan, Kibret Mequanint, Beth Gillies, Rene Harrison, Gordon Southam, Cheryle Seguin, Hong Hong Chen, Donglin Bai, Douglas Hamilton and Rony Sharon.

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  1. Department of Physics and Astronomy, The University of Western Ontario, London, ON, Canada

    Silvia Mittler

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  1. Silvia Mittler
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Correspondence to Silvia Mittler .

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  1. Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal

    Paulo A. Ribeiro

  2. Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal

    Maria Raposo

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Mittler, S. (2018). Waveguide Evanescent Field Fluorescence and Scattering Microscopy: The Status Quo. In: Ribeiro, P., Raposo, M. (eds) Optics, Photonics and Laser Technology. Springer Series in Optical Sciences, vol 218. Springer, Cham. https://doi.org/10.1007/978-3-319-98548-0_1

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