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Genomic Applications in Pathology pp 183–190Cite as

Next-Generation Sequencing for Single-Gene Analysis

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Abstract

Single-gene analysis has been the standard analytic method since DNA sequencing was originally performed. In practice, it was too time-consuming and expensive to perform analysis on more than a few exons or genes at a time. The nature of high-throughput sequencing has altered these calculations, and large panels of potentially pathogenic genes or even whole exomes or genomes are sequenced routinely. However, the techniques and equipment useful for high-throughput sequencing can be applied to single-gene targets in specialized situations. This chapter discusses these applications in minimal residual disease detection, infectious diseases, and HLA typing, among others.

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Authors and Affiliations

  1. Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Hao Ho & Christopher D. Gocke

Authors
  1. Hao Ho
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  2. Christopher D. Gocke
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Correspondence to Christopher D. Gocke .

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Editors and Affiliations

  1. Professor and Chair of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA

    George Jabboure Netto

  2. Department of Pathology and Laboratory Medicine, NorthShore University HealthSystem, Clinical Professor of Pathology, University of Chicago Pritzker School of Medicine, Evanston, IL, USA

    Karen L. Kaul

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Ho, H., Gocke, C.D. (2019). Next-Generation Sequencing for Single-Gene Analysis. In: Netto, G., Kaul, K. (eds) Genomic Applications in Pathology. Springer, Cham. https://doi.org/10.1007/978-3-319-96830-8_15

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  • DOI: https://doi.org/10.1007/978-3-319-96830-8_15

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-96829-2

  • Online ISBN: 978-3-319-96830-8

  • eBook Packages: MedicineMedicine (R0)

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