Abstract
Unused fertilizers are the single largest source of nitrous oxide greenhouse gas emissions globally, apart from causing N-pollution in ground and surface waters and eutrophication. While short-term improvements in N-use efficiency (NUE) can be made by agronomic practices, long-term crop improvement is only possible through biological interventions. The lack of clearly defined phenotype and genotype has delayed this process till recently, but the advent of omics and reverse genetics is opening up new avenues to improve crop NUE. It is becoming increasingly evident that several genes and pathways contribute to NUE. Many N-responsive regulators of root development, sensing and signaling, transportation, utilization and remobilization have been targeted to improve NUE, such as C-terminally encoded peptides (CEPs), CLAVATA3/endosperm surrounding region-related peptides (CLE), MADS-box transcription factors, and NAC transcription factors. The nitrate transporter of NRT1.1 is proposed to be a sensor or transceptor, which regulates the crop yield by acting as a component in the Ca2+-mediated signaling cascade. Several other signaling pathways involving target of rapamycin (TOR) complex, general amino acid control non-derepressible 2 (GCN2), ionotropic glutamate-like receptor (iGLR), and PII proteins have been found to play a important roles in maintaining proper N balance in plants. In cereals, cytosolic glutamine synthase, glutamate synthetase, and alanine/aspartate aminotransferases are important targets, as they are involved in remobilization of N from senescing leaves during grain filling and in maintaining proper C/N balance. Several post-transcriptional regulators such as non-coding small RNAs and post-translational regulators such as kinases and phosphatases regulate the expression level of genes involved in N-response/NUE and are emerging as novel targets.
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Abdula SE, Lee HJ, Kim J, Niño MC, Jung Y-J, Cho Y-C abd Cho Y-G (2016) BrUGE1transgenic rice showed improved growth performance with enhanced drought tolerance. Breed Sci 66(2), 226–233
Alvarez JM, Riveras E, Vidal EA, Gras DE, Contreras‐López O, Tamayo KP, Aceituno F, Gómez I, Ruffel S, Lejay L, Jordana X (2014) Systems approach identifies TGA1 and TGA4 transcription factors as important regulatory components of the nitrate response of Arabidopsis thaliana roots. The Plant J 80(1):1–3
Araya T, Miyamoto M, Wibowo J, Suzuki A, Kojima S, Tsuchiya YN, Sawa S, Fukuda H, von Wirén N, Takahashi H (2014) CLE-CLAVATA1 peptide-receptor signaling module regulates the expansion of plant root systems in a nitrogen-dependent manner. P Natl A Sci 111(5):2029–2034
Araya T, von Wirén N, Takahashi H (2016) CLE peptide signaling and nitrogen interactions in plant root development. Plant Mol Biol 91(6):607–615
Bargmann BOR, Marshall-Colon A, Efroni I, Ruffel S, Birnbaum KD, Coruzzi GM, Krouk G (2013) TARGET: A Transient Transformation System for Genome-Wide Transcription Factor Target Discovery. Mol Pla 6(3):978–980
Bellegarde F, Gojon A, Martin A (2017) Signals and players in the transcriptional regulation of root responses by local and systemic N signaling in Arabidopsis thaliana. J Exp Bot 68(10):2553–2565
Bouguyon E, Brun F, Meynard D, Kubeš M, Pervent M, Leran S, Lacombe B, Krouk G, Guiderdoni E, Zažímalová E, Hoyerová K (2015) Multiple mechanisms of nitrate sensing by Arabidopsis nitrate transceptor NRT1.1. Nat Plants 1(3):15015
Castaings L, Camargo A, Pocholle D, Gaudon V, Texier Y, Boutet‐Mercey S, Taconnat L, Renou J, Daniel‐Vedele F, Fernandez E, Meyer C, Krapp, A (2009) The nodule inception-like protein 7 modulates nitrate sensing and metabolism in Arabidopsis. The Plant J 57(3):426–435
Calatrava V, Chamizo-Ampudia A, Sanz-Luque E, Ocaña-Calahorro F, Llamas A, Fernandez E, Galvan A (2017) How Chlamydomonas handles nitrate and the nitric oxide cycle. J Exp Bot 68(10):2593–2602
Chakraborty N. and Raghuram N. (2011) Nitrate sensing and signaling in genomewide plant N response. In Nitrogen Use Efficiency in Plants, V. Jain, P. Anandakumar (eds) New India Publishing Agency, New Delhi. pp. 45–62
Chen L, Liao H (2017) Engineering crop nutrient efficiency for sustainable agriculture. J Integr Plant Biol 59(10):710–735
Chien P-S, Chiang C-B, Wang Z, Chiou T-J (2017) MicroRNA-mediated signaling and regulation of nutrient transport and utilization. Curr Opinion in Plant Biol 39:73–79
Das SK, Pathak RR, Choudhury D, Raghuram N (2007) Genomewide computational analysis of nitrate response elements in rice and Arabidopsis. Mol Genet Genomics 278(5):519–525
Dellero Y, Lamothe-Sibold M, Jossier M, Hodges M (2015) Arabidopsis thaliana ggt1 photorespiratory mutants maintain leaf carbon: nitrogen balance by reducing RuBisCO content and plant growth. The Plant J 83(6):1005–1018
Dobrenel T, Caldana C, Hanson J, Robaglia C, Vincentz M, Veit B, Meyer C (2016) TOR signaling and nutrient sensing. Ann Rev Plant Biol 67(1):261–285
Fan X, Tang Z, Tan Y, Zhang Y, Luo B, Yang M, Lian X, Shen Q, Miller AJ, Xu G (2016) Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields. P Natl A Sci 113(26):7118–7123
Fan X, Naz M, Fan X, Xuan W, Miller AJ, Xu G (2017) Plant nitrate transporters: from gene function to application. J Exp Bot 68(10):2463–2475
Forde BG (2014). Nitrogen signalling pathways shaping root system architecture: an update. Curr Opin Plant Biol 21:30–36
Forde BG, Cutler SR, Zaman N, Krysan PJ (2013) Glutamate signalling via a MEKK1 kinase-dependent pathway induces changes in Arabidopsis root architecture. The Plant J 75(1):1–10
Gaju O, Allard V, Martre P, Snape JW, Heumez E, LeGouis J, Moreau D, Bogard M, Griffiths S, Orford S, Hubbart S (2011) Identification of traits to improve the nitrogen-use efficiency of wheat genotypes. Field Crops Res 123(2):139–152
Gan Y, Bernreiter A, Filleur S, Abram B, Forde BG (2012) Overexpressing the ANR1 MADS-box gene in transgenic plants provides new insights into its role in the nitrate regulation of root development. Plant Cell Physiol 53(6):1003–1016
Gent L, Forde BG (2017) How do plants sense their nitrogen status? J Exp Bot 68(10):2531–2539
Gifford ML, Banta JA, Katari MS, Hulsmans J, Chen L, Ristova D, Tranchina D, Purugganan MD, Coruzzi GM, Birnbaum KD (2013) Plasticity regulators modulate specific root traits in discrete nitrogen environments. PLoS Genet 9(9):e1003760
Gifford ML, Dean A, Gutierrez RA, Coruzzi GM, Birnbaum KD (2017) Cell-specific nitrogen responses mediate developmental plasticity. P Natl A Sci 105(2):803–808
Gutiérrez RA, Stokes TL, Thum K, Xu X, Obertello M, Katari MS, Tanurdzic M, Dean A, Nero DC, McClung CR, Coruzzi GM (2008) Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1. P Natl A Sci 105(12):4939–4944
He X, Qu B, Li W, Zhao X, Teng W, Ma W, Ren Y, Li B, Li Z,Tong Y (2015) The nitrate-inducible NAC transcription factor TaNAC2-5A controls nitrate response and increases wheat yield. Plant Physiol 169(3):1991–2005
Havé M, Marmagne A, Chardon F, Masclaux-Daubresse C (2016) Nitrogen remobilisation during leaf senescence: lessons from Arabidopsis to crops. J Exp Bot 68(10):2513–2529
Ho CH, Lin SH, Hu HC, Tsay YF (2009) CHL1 functions as a nitrate sensor in plants. Cell 138(6):1184–1194
Hu B, Wang W, Ou S, Tang J, Li H, Che R, Zhang Z, Chai X, Wang H, Wang Y, Liang C, Liu L, Piao Z, Deng Q, Deng K, Xu C, Liang Y, Zhang L, Li L, Chu C (2015) Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies. Nature Genetics 47(7):834–838
Huang S, Chen S, Liang Z, Zhang C, Yan M, Chen J, Xu G, Fan X, Zhang Y (2015) Knockdown of the partner protein OsNAR2.1 for high-affinity nitrate transport represses lateral root formation in a nitrate-dependent manner. Sci Rep 5:18192
Klemens PAW, Patzke K, Deitmer J, Spinner L, Le Hir R, Bellini C, Bedu M, Chardon F, Krapp A, Neuhaus HE (2013) Overexpression of the vacuolar sugar carrier AtSWEET16 modifies germination, growth, and stress tolerance in Arabidopsis. Plant Physiol 163(3):1338–1352
Krapp A, David LC, Chardin C, Girin T, Marmagne A, Leprince A-S, Chaillou S, Ferrario-Méry S, Meyer C, Daniel-Vedele F (2014) Nitrate transport and signaling in Arabidopsis. J Exp Bot 65(3):789–798
Li H, Hu B, Chu C (2017) Nitrogen use efficiency in crops: lessons from Arabidopsis and rice. J Exp Bot 68(10):2477–2488
Li X, Zeng R, Liao H (2016) Improving crop nutrient efficiency through root architecture modifications. J Integr Plant Biol 58(3):193–202
Liu KH, Niu Y, Konishi M, Wu Y, Du H, Chung HS, Li L, Boudsocq M, McCormack M, Maekawa S, Ishida T (2017) Discovery of nitrate–CPK–NLP signalling in central nutrient–growth networks. Nature 545(7654):311–316
Liu W, Han X, Zhan G, Zhao Z, Feng Y, Wu C (2015) A novel sucrose-regulatory MADS-box transcription factor GmNMHC5 promotes root development and nodulation in soybean (Glycine max [L.] Merr.). Int J Mol Sci 16(9):20657–20673
Liu W, Sun Q, Wang K, Du Q, Li W-X (2016) Nitrogen limitation adaptation (NLA) is involved in source-to-sink remobilization of nitrate by mediating the degradation of NRT1.7 in Arabidopsis. New Phytol 214(2):734–744
Liu Y, von Wirén N (2017) Ammonium as a signal for physiological and morphological responses in plants. J Exp Bot 68(10):2581–2592
Lynch JP (2013) Steep, cheap and deep: an ideotype to optimize water and N acquisition by maize root systems. Ann Bot 112(2):347–357
Ma W, Li J, Qu B, He X, Zhao X, Li B, Fu X, Tong Y (2014) Auxin biosynthetic gene TAR2 is involved in low nitrogen-mediated reprogramming of root architecture in Arabidopsis. The Plant J 78(1):70–79
Marchive C, Roudier F, Castaings L, Bréhaut V, Blondet E, Colot V, Meyer C, Krapp A (2013) Nuclear retention of the transcription factor NLP7 orchestrates the early response to nitrate in plants. Nat Commun 4:1713
Młodzińska E, Kłobus G, Christensen MD, Fuglsang AT (2015) The plasma membrane H(+)-ATPase AHA2 contributes to the root architecture in response to different nitrogen supply. Physiol Plant 154(2):270–282
Mochizuki S, Jikumaru Y, Nakamura H, Koiwai H, Sasaki K, Kamiya Y, Kamiya Y, Ichikawa H, Minami E, Nishizawa Y (2014) Ubiquitin ligase EL5 maintains the viability of root meristems by influencing cytokinin-mediated nitrogen effects in rice. J Exp Bot 65(9):2307–2318
Morita S, Suga T, Yamazaki K (1988) The relationship between root length density and yield in rice plants. Jpn J Crop Sci 57(3):438–443
Murray JD, Liu C-W, Chen Y, Miller AJ (2016) Nitrogen sensing in legumes. J Exp Bot 68(8):1919–1926
Nishizawa Y, Mochizuki S, Koiwai H, Kondo K, Kishimoto K, Katoh E, Minami E (2015) Rice ubiquitin ligase EL5 prevents root meristematic cell death under high nitrogen conditions and interacts with a cytosolic GAPDH. Plant Signal Behav 10(3):e990801
Noguero M, Lacombe B (2016) Transporters involved in root nitrate uptake and sensing by Arabidopsis. Front Plant Sci (7):1391
O’Brien JA, Vega A, Bouguyon E, Krouk G, Gojon A, Coruzzi G, Gutiérrez RA (2016) Nitrate transport, sensing, and responses in plants. Mol Plant 9(6):837–856
Ohyama K, Ogawa M, Matsubayashi Y (2008) Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis. The Plant J 55(1):152–160
Okamoto S, Suzuki T, Kawaguchi M, Higashiyama T, Matsubayashi Y (2015) A comprehensive strategy for identifying long-distance mobile peptides in xylem sap. The Plant J 84(3):611–620
Okumoto S, Versaw W (2017) Genetically encoded sensors for monitoring the transport and concentration of nitrogen-containing and phosphorus-containing molecules in plants. Curr Opin Plant Biol 39:129–135
Ondzighi-Assoume CA, Chakraborty S, Harris JM (2016) Environmental nitrate stimulates abscisic acid accumulation in Arabidopsis root tips by releasing it from inactive stores. Plant Cell 28(3):729–745
Pathak RR, Ahmad A, Lochab S, Raghuram N (2008) Molecular physiology of plant N-use efficiency and biotechnological options for its enhancement. Curr Sci 94(11):1394–1403
Pathak RR, Das SK, Choudhury D, Raghuram N (2009) Genomewide bioinformatic analysis negates any specific role for Dof, GATA and Ag/cTCA motifs in nitrate responsive gene expression in Arabidopsis. Physiol Mol Biol Pla 15(2):145–150
Pathak RR, Lochab S, Raghuram N (2011) Improving nitrogen use efficiency. In Compr Biotechnol, vol 4, 2nd edn. Elsevier, Oxford, pp 209–218
Qin S, Sun X, Hu C, Tan Q, Zhao X, Xin J, Wen X (2017) Effect of NO3−:NH4+ ratios on growth, root morphology and leaf metabolism of oilseed rape (Brassica napus L.) seedlings. Acta Physiol Plant 39(9):198
Qu B, He X, Wang J, Zhao Y, Teng W, Shao A, Zhao X, Ma W, Wang J, Li B, Li Z (2015) A wheat CCAAT box-binding transcription factor increases the grain yield of wheat with less fertilizer input. Plant Physiol 167(2):411–423
Reddy MM, Ulaganathan K (2015) Nitrogen nutrition, its regulation and biotechnological approaches to improve crop productivity. Am J Plant Sci 6(18):2745–2798
Remans T, Nacry P, Pervent M, Filleur S, Diatloff E, Mounier E, Tillard P, Forde BG, Gojon A (2006) The Arabidopsis NRT1.1 transporter participates in the signaling pathway triggering root colonization of nitrate-rich patches. Proc Natl Acad Sci USA 103(50):19206–19211
Riveras E, Alvarez JM, Vidal EA, Oses C, Vega A, Gutiérrez RA (2015) The calcium ion is a second messenger in the nitrate signaling pathway of Arabidopsis 1. Plant Physiol 177(1):00961
Roberts I, Smith S, Stes E, De Rybel B, Staes A, Van De Cotte B, Njo MF, Dedeyne L, Demol H, Lavenus J, Audenaert D (2016) CEP5 and XIP1/CEPR1 regulate lateral root initiation in Arabidopsis. J Exp Bot 67(16):4889–4899
Rosas U, Cibrian-Jaramillo A, Ristova D, Banta JA, Gifford ML, Fan AH, Zhou RW, Kim GJ, Krouk G, Birnbaum KD, Purugganan MD (2013) Integration of responses within and across Arabidopsis natural accessions uncovers loci controlling root systems architecture. P Natl A Sci 110(37):15133–15138
Rothstein SJ, Bi Y-M, Coneva V, Han M, Good A (2014) The challenges of commercializing second-generation transgenic crop traits necessitate the development of international public sector research infrastructure. J Exp Bot 65(19):5673–5682
Sesma A, Castresana C, Castellano MM (2017) Regulation of translation by TOR, eIF4E and eIF2α in plants: current knowledge, challenges and future perspectives. Front Plant Sci 8:644
Shahzad Z, Amtmann A (2017) Food for thought: how nutrients regulate root system architecture. Curr Opin Plant Biol 39:80–87
Sinha VB, Jangam AP, Raghuram N (2018) Biological determinants of crop N use efficiency and biotechnological avenues for improvement. In: Masso C, Bleeker A, Raghuram N, Bekunda M, Sutton M (eds) Proceedings of the N2013. Springer
Slovak R, Göschl C, Su X, Shimotani K, Shiina T, Busch W (2014) A scalable open-source pipeline for large-scale root phenotyping of Arabidopsis. Plant Cell 26(6):2390–2403
Steffens B, Rasmussen A (2016) The physiology of adventitious roots. Plant Physiol 170(2):603–617
Sun C-H, Yu J-Q, Hu D-G (2017) Nitrate: a crucial signal during lateral roots development. Front Plant Sci 8:485
Sun H, Qian Q, Wu K, Luo J, Wang S, Zhang C, Ma Y, Liu Q, Huang X, Yuan Q, Han R, Zhao M, Dong G, Guo L, Zhu X, Gou Z, Wang W, Wu Y, Lin H, Fu X (2014) Heterotrimeric G proteins regulate nitrogen-use efficiency in rice. Nat Genet 46(6):652–656
Sun J, Ye M, Peng S, Li Y (2016) Nitrogen can improve the rapid response of photosynthesis to changing irradiance in rice (Oryza sativa L.) plants. Sci Rep 6(1):31305
Sutton MA, Bleeker A, Howard CM, Bekunda M, Grizzetti B, de Vries W, van Grinsven HJM, Abrol YP, Adhya TK, Billen G,. Davidson EA, Datta A, Diaz R, Erisman JW, Liu XJ, Oenema O, Palm C, Raghuram N, Reis S, Scholz RW, Sims T, Westhoek H, Zhang FS, with contributions from Ayyappan S, Bouwman AF, Bustamante M, Fowler D, Galloway JN, Gavito ME, Garnier J, Greenwood S, Hellums DT, Holland M, Hoysall C, Jaramillo VJ, Klimont Z, Ometto JP, Pathak H, Plocq Fichelet V, Powlson D, Ramakrishna K, Roy A, Sanders K, Sharma C, Singh B, Singh U, Yan XY, Zhang Y (2013) Our nutrient world: the challenge to produce more food and energy with less pollution. Global Overview of Nutrient Management, Centre for Ecology and Hydrology, Edinburgh on behalf of the Global Partnership on Nutrient Management and the International Nitrogen Initiative
Tabata R, Sumida K, Yoshii T, Ohyama K, Shinohara H, Matsubayashi Y (2014) Perception of root-derived peptides by shoot LRR-RKs mediates systemic N-demand signaling. Science (New York, N.Y.) 346(6207):343–346
Undurraga SF, Ibarra-Henríquez C, Fredes I, Álvarez JM, Gutiérrez RA (2017) Nitrate signaling and early responses in Arabidopsis roots. J Exp Bot 68(10):2541–2551
Vidal EA, Moyano TC, Riveras E, Contreras-López O, Gutiérrez RA (2013) Systems approaches map regulatory networks downstream of the auxin receptor AFB3 in the nitrate response of Arabidopsis thaliana roots. P Natl A Sci 110(31):12840–12845
Vidal EA, Viviana A, Lu C, Parry G, Green PJ, Coruzzi GM, Gutiérrez RA (2010) Nitrate-responsive miR393/AFB3 regulatory module controls root system architecture in Arabidopsis Thaliana. P Natl A Sci 107(9):4477–4482
Verzeaux J, Hirel B, Dubois F, Lea PJ, Tétu T (2017) Agricultural practices to improve nitrogen use efficiency through the use of arbuscular mycorrhizae: basic and agronomic aspects. Plant Sci 264:48–56
Wada S, Hayashida Y, Izumi M, Kurusu T, Hanamata S, Kanno K, Kojima S, Yamaya T, Kuchitsu K, Makino A, Ishida H (2015) Autophagy supports biomass production and nitrogen use efficiency at the vegetative stage in rice. Plant Physiol 168(1):60–73
Wan TE, Xue HE, TONG YP (2017) Transgenic approaches for improving use efficiency of nitrogen, phosphorus and potassium in crops. J Integr Agri 16 (12):60345–60347
Wang R, Tischner R, Gutiérrez RA, Hoffman M, Xing X, Chen M, Coruzzi G, Crawford NM (2004) Genomic analysis of the nitrate response using a nitrate reductase-null mutant of Arabidopsis. Plant Physiol 136(1):2512–2522
Wang X, Peng F, Li M, Yang L, Li G (2012) Expression of a heterologous SnRK1 in tomato increases carbon assimilation, nitrogen uptake and modifies fruit development. J Plant Physiol 169(12):1173–1182
Weiland M, Mancuso S, Baluska F (2014) Signalling via glutamate and GLRs in Arabidopsis thaliana. Funct Plant Biol 43(1):1–25
Xuan W, Beeckman T, Xu G (2017) Plant nitrogen nutrition: sensing and signaling. Curr Opin Plant Biol 39:57–65
Xu N, Wang R, Zhao L, Zhang C, Li Z, Lei Z, Liu F, Guan P, Chu Z, Crawford NM,Wang Y (2016) The Arabidopsis NRG2 protein mediates nitrate signaling and interacts with and regulates key nitrate regulators. The Plant Cell 28(2):485–504
York LM, Silberbush M, Lynch JP (2016) Spatiotemporal variation of nitrate uptake kinetics within the maize (Zea mays L.) root system is associated with greater nitrate uptake and interactions with architectural phenes. J Exp Bot 67(12):3763–3775
Yang JC, Zhang H, Zhang JH (2012) Root morphology and physiology in relation to the yield formation of rice. J Integr Agri 11(6):920–926
Yan Y, Wang H, Hamera S, Chen X, Fang R (2014) MiR444a has multiple functions in the rice nitrate-signaling pathway. The Plant J 78(1):44–55
Yu C, Liu Y, Zhang A, Su S, Yan A, Huang L, Ali I, Liu Y, Forde BG, Gan Y (2015) MADS-box transcription factor OsMADS25 regulates root development through affection of nitrate accumulation in rice. PLoS One 10(8):e0135196.
Yu LH, Miao ZQ, Qi GF, Wu J, Cai XT, Mao JL, Xiang CB (2014) MADS-box transcription factor AGL21 regulates lateral root development and responds to multiple external and physiological signals. Mol Plant 7:1653–1669
Yu LH, Wu J, Tang H, Yuan Y, Wang S-M, Wang Y-P, Zhu QS, Li SG, Xiang C-B (2016) Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation. Scientific reports 6:27795
Zeng D-D, Qin R, Li M, Alamin M, Jin X-L, Liu Y, Shi C-H (2016) The ferredoxin-dependent glutamate synthase (OsFd-GOGAT) participates in leaf senescence and the nitrogen remobilization in rice. Mol Genet Genomics 292(2):385–395
Zhang H, Forde BG (1998) An Arabidopsis MADS box gene that controls nutrient-induced changes in root architecture. Science (New York, N.Y.) 279(5349):407–409
Zhang X, Davidson EA, Mauzerall DL, Searchinger TD, Dumas P, Shen Y (2015) Managing nitrogen for sustainable development. Nature 528(7580):51
Zuluaga DL, De Paola D, Janni M, Curci PL, Sonnante G (2017) Durum wheat miRNAs in response to nitrogen starvation at the grain filling stage. PLoS One 12(8):e0183253
Acknowledgements
This work was supported in part by research grant to NR and fellowship to NS from the Department of Biotechnology, Govt. of India under the Indo-UK Virtual Nitrogen Centre on Nitrogen Efficiency of Whole cropping Systems (NEWS) BT/IN/UK-VNC/44/NR/2015-16. VM is a recipient of DBT fellowship (BCIL/HRD/DBT-JRF/FLSP).
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Mandal, V.K., Sharma, N., Raghuram, N. (2018). Molecular Targets for Improvement of Crop Nitrogen Use Efficiency: Current and Emerging Options. In: Shrawat, A., Zayed, A., Lightfoot, D. (eds) Engineering Nitrogen Utilization in Crop Plants. Springer, Cham. https://doi.org/10.1007/978-3-319-92958-3_5
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