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Using Electrically Tunable Lens to Improve Axial Resolution and Imaging Field in Light Sheet Fluorescence Microscope

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The Proceedings of the International Conference on Sensing and Imaging (ICSI 2017)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 506))

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Abstract

Light sheet fluorescence microscope (LSFM) has become one of the most promising 3D microscopy techniques due to its prominent lateral section illumination. In addition, comparing with the confocal microscope, it has low phototoxicity and high temporal resolution. In this paper, we proposed an easy-to-operate tiling light sheet fluorescence microscope, which integrates a cylindrical lens, a galvanometric mirror, and an excitation objective to produce a light sheet with desired numerical aperture and axial resolution and utilizes an electrically tunable lens (ETL) to enlarge the limited field of view (FOV) of single excitation section following the temporal multiplexing. Imaging performances have been characterized from the experiment by imaging the 20 nm fluorescent beads with water immersion, and the benefits of our proposed technique have been shown in volumetric resolution, FOV, and speed as well as contrast.

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References

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Authors and Affiliations

  1. Beijing Key Lab. for Precision Optoelectronic Measurement Instrument and Technology, School of Opto-Electronics, Beijing Institute of Technology, Beijing, China

    Muyue Zhai, Qiudong Zhu & Shanshan Wang

  2. Institute of Molecular Medicine, Peking University, Beijing, China

    Xiaoshuai Huang

  3. LMAM, School of Mathematical Sciences, Peking University, Beijing, China

    Heng Mao

Authors
  1. Muyue Zhai
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  2. Xiaoshuai Huang
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  3. Heng Mao
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  4. Qiudong Zhu
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  5. Shanshan Wang
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Corresponding author

Correspondence to Shanshan Wang .

Editor information

Editors and Affiliations

  1. School of Mathematical Sciences, Peking University, Beijing, China

    Ming Jiang

  2. Department of Electrical and Computer Engineering, The University of Akron, Akron, OH, USA

    Nathan Ida

  3. Institute of Applied Mathematics, Saarland University, Saarbrücken, Germany

    Alfred K. Louis

  4. Department of Mathematics, Tufts University, Medford, MA, USA

    Eric Todd Quinto

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Zhai, M., Huang, X., Mao, H., Zhu, Q., Wang, S. (2019). Using Electrically Tunable Lens to Improve Axial Resolution and Imaging Field in Light Sheet Fluorescence Microscope. In: Jiang, M., Ida, N., Louis, A., Quinto, E. (eds) The Proceedings of the International Conference on Sensing and Imaging. ICSI 2017. Lecture Notes in Electrical Engineering, vol 506. Springer, Cham. https://doi.org/10.1007/978-3-319-91659-0_34

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  • DOI: https://doi.org/10.1007/978-3-319-91659-0_34

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-91658-3

  • Online ISBN: 978-3-319-91659-0

  • eBook Packages: EngineeringEngineering (R0)

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