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Metallomics in Fish

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Metallomics

Part of the book series: Advances in Experimental Medicine and Biology ((PMISB,volume 1055))

Abstract

Metallomics allows the integration of traditionally analytical studies with inorganic and biochemical studies. The study of metallomics in living organisms allows us to obtain information about how the metal ion is distributed and coordinated with proteins, the essentiality and/or toxicity, and the individual concentrations of metal species, thus contributing to elucidation of the physiological and functional aspects of these biomolecules. In this context, several lines of research have appeared in the literature with different terms and approaches. For example, metallomic, which deals with the characterization of the total metal/metalloid species present in an organism; metalloprotein, which deals with the characterization of the total elements present in a specific site of an organism (cellular behavior, protein, metalloprotein); and metallomic, which deals with a more in-depth study of metallome. In this area, information is sought on the interactions and functional connections of metal/metalloid species with genes, proteins, metabolites and other biomolecules of the organism and, therefore, the elucidation of the biological role exerted by the metal ions bound to the biomolecules. In this chapter, we will describe techniques used in animal studies.

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Abbreviations

2D-PAGE:

Two-dimensional polyacrylamide gel electrophoresis

AAS:

Atomic absorption spectrometry

ESI:

Electrospray ionization

ESI-MS/MS:

Electrospray ionization tandem mass spectrometry

FAAS:

Flame atomic absorption spectrometry

GFAAS:

Graphite furnace atomic absorption spectrometry

ICP-MS:

Inductively coupled plasma source mass spectrometry

IEF:

Isoelectric focusing

LC:

Multidimensional liquid chromatography

MALDI:

Matrix assisted laser desorption ionization

MALDI-TOF /MS:

Time-of-flight mass spectrometry coupled to laser-assisted matrix

Mm:

Molecular mass

MS:

Mass spectrometry

PAGE:

One-dimensional polyacrylamide gel electrophoresis

pI:

Isoeletric point

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SEC:

size-exclusion chromatography

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Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA

    Camila Pereira Braga & Jiri Adamec

  2. Department of Chemistry and Biochemistry, Institute of Bioscience, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil

    Pedro de Magalhães Padilha

Authors
  1. Camila Pereira Braga
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  2. Jiri Adamec
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  3. Pedro de Magalhães Padilha
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Corresponding author

Correspondence to Pedro de Magalhães Padilha .

Editor information

Editors and Affiliations

  1. Universidade Estadual de Campinas – Unicamp, Institute of Chemistry, São Paulo, Brazil

    Marco Aurélio Zezzi Arruda

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Braga, C.P., Adamec, J., de Magalhães Padilha, P. (2018). Metallomics in Fish. In: Arruda, M. (eds) Metallomics. Advances in Experimental Medicine and Biology(), vol 1055. Springer, Cham. https://doi.org/10.1007/978-3-319-90143-5_5

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