Abstract
The cork oak is an evergreen tree species typical of Mediterranean forests. It grows in dense stands but also in low density, in the savanna-like system called “dehesa” in Spain and “montado” in Portugal. The most characteristic production of Quercus suber is cork, although acorn crops are also important for feeding pigs of the Iberian race. The products obtained from the species justify the accomplishment of genetic improvement programs. Current strategies of forest tree breeding emphasize on the use of vegetative propagation to rapidly capture all the potential of selected individuals and establish them in plantations, performing Multi-Varietal Forestry . Somatic embryogenesis is one of the enabling technologies to carry out that strategy. Our protocol for cloning adult cork oak trees by somatic embryogenesis is robust enough to capture any genotype, and currently it is adequate to produce the limited number of plants needed to establish clonal test. However for large-scale profitable clonal propagation, the scaling-up of plant production using culture in liquid medium has to be developed. Herein we describe step-wise protocols to perform the production of somatic embryos obtained from selected Quercus suber trees using culture systems based on liquid medium.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Alegre J, Alonso-Blázquez N, Jiménez J, Hernández I, Ruiz M, Celestino C, Toribio M (2011) Propagation of cork oak selected trees by somatic embryogenesis: from solid to liquid medium. In: Park YS, Bonga JM, Park SY, Moon, HK (eds) Proceedings 1st international conference of the IUFRO working party 2.09.02 “advances in somatic embryogenesis of trees and its application for the future forests and plantations”, 19–21 Aug 2010, Suwon, Republic of Korea. pp 44–49, ISBN 978-89-8176-819-5
Álvarez JRG (2016) The image of a tamed landscape: dehesa through History in Spain. Cult Hist Digital J 5(1):e003. https://doi.org/10.3989/chdj.2016.003
Aroso IM, Araújo AR, Fernandes JP, Santos T, Batista MT, Pires RA, Mano JF, Reis RL (2017) Hydroalcoholic extracts from the bark of Quercus suber L. (Cork): optimization of extraction conditions, chemical composition and antioxidant potential. Wood Sci Technol. https://doi.org/10.1007/s00226-017-0904-y
Bejarano I, Godoy-Cancho B, Franco L, Martínez-Cañas MA, Tormo MA (2015) Quercus suber L. cork extracts induce apoptosis in human myeloid leukaemia HL-60 cells. Phytother Res 29:1180–1187. https://doi.org/10.1002/ptr.5364
Ben Ali N, Lamarti A (2014) Macronutrients effect on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Am J Plant Sci 5:1851–1861. https://doi.org/10.4236/ajps.2014.513199
Corredoira E, Toribio M, Vieitez AM (2014) Clonal propagation via somatic embryogenesis in Quercus spp. In: Ramawat KG, Mérillon J-M, Ahuja MR (eds) Tree biotechnology. CRC Press, Boca Raton, FL, pp 262–302. ISBN 978-1-4665-9714-3
Costa A, Madeira M, Plieninger T (2014) Cork oak woodlands patchiness: a signature of imminent deforestation? Appl Geogr 54:18–26. https://doi.org/10.1016/j.apgeog.2014.07.006
Custódio L, Patarra J, Alberício F, da Rosa Neng N, Nogueira JMF, Romano A (2015) Phenolic composition, antioxidant potential and in vitro inhibitory activity of leaves and acorns of Quercus suber on key enzymes relevant for hyperglycemia and Alzheimer’s disease. Ind Crops Prod 64:45–51. https://doi.org/10.1016/j.indcrop.2014.11.001
de Sampaio e Paiva Camilo-Alves C, da Clara MIE, de Almeida Ribeiro NMC (2013) Decline of Mediterranean oak trees and its association with Phytophthora cinnamomi: a review. Eur J For Res 132:411–432. https://doi.org/10.1007/s10342-013-0688-z
Duarte AP, Bordado JC (2015) Cork–a renewable raw material: forecast of industrial potential and development priorities. Front Mater 2(2):1–8. https://doi.org/10.3389/fmats.2015.00002
Ducos JP, Labbe G, Lambot C, Pétiard V (2007) Pilot scale process for the production of pre-germinated somatic embryos of selected robusta (Coffea canephora) clones. In Vitro Cell Develop Biol Plant 43:652–659. https://doi.org/10.1007/s11627-007-9075-0
Etienne H, Bertrand B, Georget F, Lartaud M, Montes F, Dechamp E, Verdeil JL, Barry-Etienne D (2013) Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects. Tree Physiol 33:640–653. https://doi.org/10.1093/treephys/tpt034
Fernández-Guijarro B, Celestino C, Toribio M (1995) Influence of external factors on secondary embryogenesis and germination in somatic embryos from leaves of Quercus suber. Plant Cell Tiss Organ Cult 41:99–106. https://doi.org/10.1007/BF00051578
Gil L, Varela MC (2008) EUFORGEN technical guidelines for genetic conservation and use for cork oak (Quercus suber). Bioversity International, Rome, Italy, 6p
Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum. Planta 107:161–170. https://doi.org/10.1007/BF00387721
Hernández I, Celestino C, Alegre J, Toribio M (2003) Vegetative propagation of Quercus suber L. by somatic embryogenesis. II. Plant regeneration from selected cork oak trees. Plant Cell Rep 21:765–770. https://doi.org/10.1007/s00299-003-0604-y
Hernández I, Cuenca B, Carneros E, Alonso-Blázquez N, Ruiz M, Celestino C, Ocaña L, Alegre J, Toribio M (2011) Application of plant regeneration of selected cork oak trees by somatic embryogenesis to implement multivarietal forestry for cork production. In: Nageswara-Rao M, Soneji JR (eds) Focus on tree micropropagation and tissue culture. Tree and forestry science and biotechnology 5 (Special Issue 1), pp 19–26. ISSN 1752–3753. http://www.globalsciencebooks.info/Online/GSBOnline/images/2011/TFSB_5(SI1)/TFSB_5(SI1)19-26o.pdf
Jiménez J (2013) Obtención de clones de alcornoque (Quercus suber L.) mediante embriogénesis somática: aplicación de medios líquidos y biorreactores en la sincronización de los procesos de desarrollo y maduración de los embriones. Ph.D. Thesis. Polytechnic University of Madrid, Spain. http://oa.upm.es/21429/1/JESUS_JIMENEZ_GARCIA.pdf
Jiménez JA, Alonso-Blázquez N, López-Vela D, Celestino C, Toribio M, Alegre J (2011) Influence of culture vessel characteristics and agitation rate on gaseous exchange, hydrodynamic stress, and growth of embryogenic cork oak (Quercus suber L.) cultures. In Vitro Cell Dev Biol Plant 47:578–588. https://doi.org/10.1007/s11627-011-9399-7
Jiménez J, López-Vela D, Ruiz-Galea M, Celestino C, Toribio M, Alegre J (2013) Embryogenic suspensions of cork oak: the first step towards mass propagation. Trees 27:13–23. https://doi.org/10.1007/s00468-012-0763-y
Lelu-Walter MA, Thompson D, Harvengt L, Sanchez L, Toribio M, Pâques LE (2013) Somatic embryogenesis in forestry with a focus on Europe: state-of-the-art, benefits, challenges and future direction. Tree Genet Genomes 9:883–899. https://doi.org/10.1007/s11295-013-0620-1
Merkle SA, Parrott WA, Flinn BS (1995) Morphogenic aspects of somatic embryogenesis. In: Thorpe TA (ed) In vitro embryogenesis in plants. Current plant science and biotechnology in agriculture, vol 20. Kluwer, Dordrecht, pp 155–203. https://doi.org/10.1007/978-94-011-0485-2_5
Moiteiro C, Marcelo Curto MJ, Mohamed N, Bailén M, Martínez-Díaz R, González-Coloma A (2006) Biovalorization of friedelane triterpenes derived from cork processing industry byproducts. J Agric Food Chem 54:3566–3571. https://doi.org/10.1021/jf0531151
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Niedz RP (2006) Regeneration of somatic embryos from sweet orange (C. sinensis) protoplasts using semi-permeable membranes. Plant Cell Tiss Organ Cult 84:353–357. https://doi.org/10.1007/s11240-005-9028-4
Park Y-S, Beaulieu J, Bousquet J (2016) Multi-varietal forestry integrating genomic selection and somatic embryogenesis. In: Park Y-S, Bonga JM, Moon H-K (eds) Vegetative propagation of forest trees. National Institute of Forest Science (NiFos), Seoul, Korea, pp 302–322
Pérez M, Bueno MA, Escalona M, Toorop P, Rodríguez R, Cañal MJ (2013) Temporary immersion systems (RITA®) for the improvement of cork oak somatic embryogenic culture proliferation and somatic embryo production. Trees 27:1277–1284. https://doi.org/10.1007/s00468-013-0876-y
Pugliese C, Sirtori F (2012) Quality of meat and meat products produced from Southern European pig breeds. Meat Sci 90:511–518. https://doi.org/10.1016/j.meatsci.2011.09.019
Puigderrajols P, Fernández-Guijarro B, Toribio M, Molinas M (1996) Origin and early development of secondary embryos in Quercus suber L. Int J Plant Sci 157:674–684. https://doi.org/10.1086/297389
Ruiz-Galea M, Alegre J, Celestino C, Toribio M (2011) Clonación y conservación de árboles singulares de la Comunidad de Madrid. Foresta 52:486–493. ISSN: 1575–2356. https://www.researchgate.net/publication/266137747_Clonacion_y_conservacion_de_arboles_singulares_de_la_Comunidad_de_Madrid
Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell culture. Can J Bot 50:199–204. https://doi.org/10.1139/b72-026
Schirone B, Spada F, Simeone MC, Vessella F (2015) Quercus suber distribution revisited. In: Box EO, Kazue Fujiwara K (eds) Warm-temperate deciduous forests around the northern hemisphere. Springer International Publishing, Switzerland, pp 181–212. https://doi.org/10.1007/978-3-319-01261-2_11
Toribio M, Celestino C, Molinas M (2005) Cork oak, Quercus suber L. In: Jain SM, Gupta PK (eds) Protocol for somatic embryogenesis in woody plants, vol 77. Forestry sciences. Springer, Dordrecht, The Netherlands, pp 445–457. https://doi.org/10.1007/1-4020-2985-3_35
Vinha AF, Barreira JCM, Costa ASG, Oliveira MBPP (2016) A New Age for Quercus spp. fruits: review on nutritional and phytochemical composition and related biological activities of acorns. Compr Rev Food Sci Food Saf 15:947–981. https://doi.org/10.1111/1541-4337.12220
Acknowledgments
Funds were provided by The Spanish National R&D Program, projects AGL2010-22292-C03-01 and AGL2013-47400-C4-1-R.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer International Publishing AG, part of Springer Nature
About this chapter
Cite this chapter
Ruiz-Galea, M. et al. (2018). Cork Oak Quercus suber L. Embryogenic Liquid Cultures. In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 84. Springer, Cham. https://doi.org/10.1007/978-3-319-89483-6_18
Download citation
DOI: https://doi.org/10.1007/978-3-319-89483-6_18
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-89482-9
Online ISBN: 978-3-319-89483-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)