Abstract
Japanese black pine (Pinus thunbergii Parl. ) and Japanese red pine (P. densiflora Zieb. et Zucc.) locally named “kuromatsu” and “akamatsu”, respectively, are two important forest tree species widely used for reforestation and landscaping in Japan. In addition, P. thunbergii is also planted along coastal areas to prevent sand movement, erosion, and damage by salt spray, and P. densiflora is also important as host species of the very expensive “matsutake” mushroom, Tricholoma matsutake.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Akiba M, Nakamura K (2005) Susceptibility of adult trees of the endangered species Pinus armandii var amamiana to pine wilt disease in the field. J For Res 10:3–7
Bonga JM (2016) Conifer clonal propagation in tree improvement programs. In: Park YS, Bonga JM, Moon HK (eds) Vegetative propagation of forest trees. NIFOS, Seoul, Korea, pp 3–31
Hay EI, Charest PJ (1999) Somatic embryo germination and desiccation tolerance in conifers. In: Jain SM, Gupta PK, Newton RJ (eds) Somatic embryogenesis in woody plants, vol 4. Kluwer Academic Publishers, Dordrecht, pp 61–96
Hosoi Y, Ishii K (2001) Somatic embryogenesis and plantlet regeneration in Pinus armandii var. amamiana. In: Morohoshi N, Komamine A (eds) Molecular breeding of woody plants. Elsevier Science, Amsterdam, pp 313–318
Hosoi Y, Maruyama TE (2012) Plant regeneration from embryogenic tissue of Pinus luchuensis Mayr, an endemic species in Ryukyu Island, Japan. Plant Biotech 29:401–406
Hosoi Y, Maruyama TE (2016) Somatic embryogenesis in sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.). In: Mujib A (ed) Somatic embryogenesis in ornamentals and its applications. Springer, India, pp 41–53
Igasaki T, Sato T, Akashi N, Mohri T, Maruyama E, Kinoshita I, Walter C, Shinohara K (2003) Somatic embryogenesis and plant regeneration from immature zygotic embryos of Cryptomeria japonica D. Don. Plant Cell Rep 22:239–243
Ishii K, Maruyama E, Hosoi Y (2001) Somatic embryogenesis of Japanese conifers. In: Morohoshi N, Komamine A (eds) Molecular breeding of woody plants. Elsevier Science, Amsterdam, pp 297–304
Kanetani S, Akiba M, Nakamura K, Gyokusen K, Saito A (2001) The process of decline of an endangered tree species, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, on the southern slope of Mt. Hasa-dake in Yaku-shima Island. J For Res 6:307–310
Kanzaki N, Aikawa T, Maehara N, Ichihara Y (2011) An inoculation experiment of Japanese Bursaphelenchus nematodes on Japanese black and red pine, Pinus thunbergii and P. densiflora. J For Res 16:325–330
Kim YW, Moon HK (2014) Enhancement of somatic embryogenesis and plant regeneration in Japanese red pine. Plant Biotechnol Rep 8:259–266
Klimaszewska K, Cyr DR (2002) Conifer somatic embryogenesis: I. Development. Dendrobiology 48:31–39
Klimaszewska K, Trontin JF, Becwar MR, Devillard C, Park YS, Lelu-Walter MA (2007) Recent progress in somatic embryogenesis of four Pinus spp. Tree For Sci Biotech 1:11–25
Kosaka H, Aikawa T, Ogura N, Tabata K, Kiyohara T (2001) Pine wilt disease caused by the pine wood nematode: the induced resistance of pine trees by the avirulent isolates of nematode. Eur J Plant Pathol 107:667–675
Kuroda K (2004) Inhibiting factors of symptom development in several Japanese red pine (Pinus densiflora) families selected as resistant to pine wilt. J For Res 9:217–224
Maruyama TE, Hosoi Y (2012a) Somatic embryogenesis and efficient plant regeneration in Japanese cypresses. In: Sato K (ed) Embryogenesis. In Tech, Rijeka, Croatia, pp 387–402
Maruyama TE, Hosoi Y (2012b) Post-maturation treatments improves and synchronizes somatic embryo germination of three species of Japanese pines. Plant Cell Tissue Organ Cult 110:45–52
Maruyama TE, Hosoi Y (2014) Plant production in Japanese pines via somatic embryogenesis. In: Ramawat KG, Merillon JM, Ahuja MR (eds) Tree biotechnology. CRC Press, Florida, pp 251–261
Maruyama TE, Hosoi Y (2016a) Somatic embryogenesis and plant propagation in Japanese black pine (Pinus thunbergii Parl.) and Japanese red pine (Pinus densiflora Zieb. et Zucc.). In: Park YS, Bonga JM, Moon HK (eds) Vegetative propagation of forest trees. Korea, NIFOS, Seoul, pp 623–638
Maruyama TE, Hosoi Y (2016b) Somatic embryogenesis in Japanese black pine (Pinus thunbergii Parl). In: Mujib A (ed) Somatic embryogenesis in ornamentals and its applications. Springer, India, pp 27–39
Maruyama E, Tanaka T, Hosoi Y, Ishii K, Morohoshi N (2000) Embryogenic cell culture, protoplast regeneration, cryopreservation, biolistic gene transfer and plant regeneration in Japanese cedar (Cryptomeria japonica D. Don). Plant Biotech 17:281–296
Maruyama E, Hosoi Y, Ishii K (2002) Somatic embryogenesis in sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.) for stable and efficient plant regeneration, propagation and protoplast culture. J For Res 7:23–34
Maruyama E, Hosoi Y, Ishii K (2005a) Somatic embryo production and plant regeneration of Japanese black pine (Pinus thunbergii). J For Res 10:403–407
Maruyama E, Hosoi Y, Ishii K (2005b) Propagation of Japanese red pine (Pinus densiflora Zieb. et Zucc.). Prop Ornam Plants 4:199–204
Maruyama E, Ishii K, Hosoi Y (2005c) Efficient plant regeneration of hinoki cypress (Chamaecyparis obtusa Sieb. et Zucc.) via somatic embryogenesis. J For Res 10:73–77
Maruyama E, Hosoi Y, Ishii K (2007) Somatic embryogenesis and plant regeration in yakutanegoyou, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endemic and endangered species in Japan. In Vitro Cell Dev Biol-Plant 43:28–34
Mota MM, Braasch H, Bravo MA, Penas AC, Burgermeister W, Metge K, Sousa E (1999) First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1:727–734
Nakagawa R, Kurushima M, Matsui M, Nakamura R, Kubo T, Funada R (2011) Polyamines promote the development of embryonal-suspensor masses and the formation of somatic embryos in Picea glehnii. In Vitro Cell Dev Bio-Plant 47:480–487
Ogita S, Ishikawa H, Kubo T, Sasamoto H (1999) Somatic embryogenesis from immature and mature zygotic embryos of Cryptomeria japonica I: embryogenic cell induction and its morphological characteristics. J Wood Sci 45:87–91
Park YS (2002) Implementation of conifer somatic embryogenesis in clonal forestry: technical requirements and deployment considerations. Ann For Sci 59:651–656
Park YS, Barrett JD, Bonga JM (1998) Application of somatic embryogenesis in high-value clonal forestry: deployment, genetic control, and stability of cryopreserved clones. In Vitro Cell Dev Biol-Plant 34:231–239
Roberts DR, Sutton BCS, Flinn BS (1990) Synchronous and high frequency germination of interior spruce somatic embryos following partial drying at high relative humidity. Can J Bot 68:1086–1090
Roberts DR, Lazaroff WR, Webster FB (1991) Interaction between maturation and high relative humidity treatments and their effects on germination of Sitka spruce somatic embryos. J Plant Physiol 138:1–6
Shoji M, Sato H, Nakagawa R, Funada R, Kubo T, Ogita S (2006) Influence of osmotic pressure on somatic embryo maturation in Pinus densiflora. J For Res 11:449–453
Stasolla C, Yeung EC (2003) Recent advances in conifer somatic embryogenesis: improving somatic embryo quality. Plant Cell Tissue Organ Cult 74:15–35
Taniguchi T (2001) Plant regeneration from somatic embryos in Pinus thunbergii (Japanese black pine) and Pinus densiflora (Japanese red pine). In: Morohoshi N, Komamine A (eds) Molecular breeding of woody plants. Elsevier Science, Amsterdam, pp 319–324
Taniguchi T, Kondo T (2000) Difference in the ability of initiation and maintenance of embryogenic cultures among Sugi (Cryptomeria japonica D. Don) seed families. Plant Biotech 17:159–162
Taniguchi T, Kurita M, Itahana N, Kondo T (2004) Somatic embryogenesis and plant regeneration from immature zygotic embryos of hinoki cypress (Chamaecyparis obtusa Sieb et Zucc.). Plant Cell Rep 23:26–31
Texeira da Silva JA, Malabadi RB (2012) Factors affecting somatic embryogenesis in conifers. J For Res 23:503–515
Togashi K, Shigesada N (2006) Spread of the pinewood nematode vectored by the Japanese pine sawyer: modeling and analytical approaches. Popul Ecol 48:271–283
Trontin JF, Hargraves C, Montalban IA, Moncalean P, Reeves C, Quoniou S, Lelu-Walter MA, Klimaszewska K (2016) International effort to induce somatic embryogenesis in adult pine trees. In: Park YS, Bonga JM, Moon HK (eds) Vegetative propagation of forest trees. NIFOS, Seoul, Korea, pp 211–260
Acknowledgements
This work was supported in part by JSPS KAKENHI Grant Number JP16K14949.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer International Publishing AG, part of Springer Nature
About this chapter
Cite this chapter
Maruyama, T.E., Hosoi, Y. (2018). Protocol for Somatic Embryogenesis in Japanese Black Pine (Pinus thunbergii Parl.) and Japanese Red Pine (Pinus densiflora Sieb. et Zucc.). In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 84. Springer, Cham. https://doi.org/10.1007/978-3-319-89483-6_17
Download citation
DOI: https://doi.org/10.1007/978-3-319-89483-6_17
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-89482-9
Online ISBN: 978-3-319-89483-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)