Abstract
Maritime pine (Pinus pinaster Aiton) is the most abundant conifer in the Mediterranean basin. Currently, maritime pine is considered to be a model conifer species for study of the adaption responses to drought stress from a genomics approach. In this context, the availability of protocols that allow not only mass vegetative propagation of selected families or genotypes, but also facilitate the functional analyses needed to verify and further to study the effects of candidate genes are necessary. Here we describe an improved protocol to generate maritime pine plants through somatic embryogenesis from immature megagametophytes. Accurate procedures for explant preparation, somatic embryo induction, proliferation, cryopreservation, maturation, germination, plant formation, and acclimatization are described.
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Acknowledgements
This work is being supported by the research projects cofinanced by the MINECO (Spanish Government) and the EU (AGL2013-47400-C4-04-R; AGL2016-76143-C4-1-R) and by predoctoral contracts to M.C. (University of Valencia) and M.M. (MINECO). Plant material supply by the Generalitat Valenciana (Centro para la Investigación y Experimentacion Forestal) by the Unit of Forest Genetic Resources (MAPAMA), TRAGSA and Servicio de Montes, Xunta de Galicia is acknowledged.
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Cano, M. et al. (2018). Maritime Pine Pinus Pinaster Aiton. In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 84. Springer, Cham. https://doi.org/10.1007/978-3-319-89483-6_13
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DOI: https://doi.org/10.1007/978-3-319-89483-6_13
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