Skip to main content
SpringerLink
Log in
Book cover

Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants pp 349–368Cite as

Haploid Embryogenesis in Tea

  • Chapter
  • First Online:

Part of the book series: Forestry Sciences ((FOSC,volume 85))

Abstract

History unveils decades of strenuous efforts made by mankind to strengthen agricultural productivity and attain sustainable food security at global level. Traditional breeding practices have been followed by plant breeders to attain genetic improvement in plants and to ease down the challenges faced by local farmers. However, there are limitations to implement these conventional breeding practices to improve woody perennials due to their long reproductive cycle and out breeding nature. Plant tissue culture techniques has emanated as a potential branch of biotechnology to help plant breeders in addressing long existing problems of inbreeding depression, prolong juvenile period and extreme heterozygosity encountered in majority of perennial crops and recalcitrant woody trees. Gamete biology, involving in vitro haploid plant production via embryogenesis from immature explants of gametic origin in anther or ovary cultures, routes easier, single step procedure of obtaining doubled haploid (pure breeding) lines within reduced time duration. This chapter accentuates the progress achieved in the field of haploid plant induction using in vivo and in vitro means and brings together the research updates on recent strategies deployed to induce diploidization of haploids and cytological and molecular basis of their identification.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Akula A, Becker D, Bateson M (2000) High-yielding repetitive somatic embryogenesis and plant recovery in a selected tea clone, ‘TRI-2025’, by temporary immersion. Plant Cell Rep 19:1140–1145

    Article  CAS  Google Scholar 

  • Alan AR, Mutschler MA, Brants A, Cobb E, Earle ED (2003) Production of gynogenic plants from hybrids of Allium cepa L. and A. roylei Stearn. Plant Sci 165:1201–1211

    Article  CAS  Google Scholar 

  • Bartošová Z, Obert B, Takacomá Š, Kormutak A, Pretová A (2005) Using enzyme polymorphism to identify the gametic origin of flax regenerants. Acta Biol Cracov Bot 47:173–178

    Google Scholar 

  • Bhatt JG, Murthy HN (2007) Factors affecting in vitro gynogenic haploid production in niger (Guizotia abyssinica (L. f.) Cass.). Plant Growth Regul 52:241–248

    Article  CAS  Google Scholar 

  • Bhojwani SS, Razdan MK (1996) Haploid Production. Plant tissue culture: theory and practice. Elsevier Scientific Publishers, Amsterdam, The Netherlands, pp 113–142

    Google Scholar 

  • Blakeslee AF, Belling J, Farnham ME, Berner AD (1922) A haploid mutant in Jimson weed, Dhatura stramonium. Science 55:646–647

    Article  CAS  PubMed  Google Scholar 

  • Bohanec B (2009) Doubled haploids via gynogenesis. In: Touraev A, Forster BP, Jain SM (eds) Advances in haploid production in higher plants. Springer, Dordrecht, Netherlands, pp 35–46

    Chapter  Google Scholar 

  • Bueno MA, Gómez A, Boscaiu M, Manzanera JA, Vicente O (1997) Stress-induced formation of haploid plants through anther culture in cork oak (Quercus suber). Physiol Plant 99:335–341

    Article  CAS  Google Scholar 

  • Chaturvedi R, Razdan MK, Bhojwani SS (2003) Production of haploids of neem (Azadirachta indica A. Juss.) by anther culture. Plant Cell Rep 21:531–537

    PubMed  CAS  Google Scholar 

  • Chen JF, Cui L, Malik AA, Mbira KG (2010) In vitro haploid and dihaploid production via unfertilized ovule culture. Plant Cell Tissue Organ Cult 104:311–319

    Article  Google Scholar 

  • Chen Y, Kenaschuk E, Dribnenki P (1998) High frequency of plant regeneration from anther culture in flax, Linum usitatissimum L. Plant Breed 117:463–467

    Article  CAS  Google Scholar 

  • Clapham D (1971) In vitro development of callus from the pollen of Lolium and Hordeum. Z Pflanzenziichtg. 65:285–292

    Google Scholar 

  • Comai L (2014) Genome elimination: translating basic research into a future tool for plant breeding. PLoS Biol 12:e1001876

    Article  PubMed  PubMed Central  Google Scholar 

  • Custers JB, Cordewener JH, Nöllen Y, Dons HJ, Campagne MM (1994) Temperature controls both gametophytic and sporophytic development in microspore cultures of Brassica napus. Plant Cell Rep 13:267–271

    Article  CAS  PubMed  Google Scholar 

  • Datta SK (2005) Androgenic haploids: factors controlling development and its application in crop improvement Curr Sci 1870–1878

    Google Scholar 

  • Da Silva DJC (2001) Effect of incubation temperature regimes and culture medium on broccoli microspore culture embryogenesis. Euphytica 119:389–394

    Article  Google Scholar 

  • Dunwell JM, Cornish M, De Courcel AGL (1985) Influence of genotype, plant growth temperature and anther incubation temperature on microspore embryo production in Brassica napus ssp. oleifera. J Exp Bot 36:679–689

    Article  Google Scholar 

  • Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8:377–424

    Article  CAS  PubMed  Google Scholar 

  • Dwivedi SL, Britt AB, Tripathi L, Sharma S, Upadhyaya HD, Ortiz R (2015) Haploids: constraints and opportunities in plant breeding. Biotechnol Adv 33:812–829

    Article  PubMed  Google Scholar 

  • Ferrie AMR, Caswell KL (2011) Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell Tissue Organ Cult 104:301–309

    Article  Google Scholar 

  • Forster BP, Heberle-Bors E, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12:368–375

    Article  CAS  PubMed  Google Scholar 

  • Gaillard A, Vergne P, Beckert M (1991) Optimization of maize microspore isolation and culture conditions for reliable plant regeneration. Plant Cell Rep 10:55–58

    Article  CAS  PubMed  Google Scholar 

  • Germanà MA (2005) Protocol of somatic embryogenesis from Citrus spp. anther culture. In: Jain SM, Gupta PK (eds) Protocol for somatic embryogenesis in woody plants. Springer, Netherlands, pp 191–207

    Chapter  Google Scholar 

  • Germana MA (2006) Doubled haploid production in fruit crops. Plant Cell Tissue Organ Cult 86:131–146

    Article  Google Scholar 

  • Germanà MA (2011) Anther culture for haploid and doubled haploid production. Plant Cell Tissue Organ Cult 104:283–300

    Article  Google Scholar 

  • Gharyal PK, Maheshwari SC (1982) In vitro differentiation of plantlets from tissue cultures of Albizzia lebbeck L. Plant Cell Tissue Organ Cult 2(1):49–53

    Article  Google Scholar 

  • Gharyal PK, Rashid A, Maheshwari SC (1983) Androgenic response from cultured anthers of a leguminous tree Cassia siamea Lam. Protoplasma 118:91–93

    Article  Google Scholar 

  • Gresshoff PM, Doy CH (1972) Haploid Arabidopsis thaliana callus and plants from anther culture. Aust J Biol Sci 25:259–264

    Article  Google Scholar 

  • Guha S, Maheswari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497

    Article  Google Scholar 

  • Guha S, Maheswari SC (1966) Cell division and differentiation of embryos in the pollen grains of Datura in vitro. Natur 212:97–98

    Article  Google Scholar 

  • Hazarika RR, Chaturvedi R (2013) Establishment of dedifferentiated callus of haploid origin from unfertilized ovaries of tea (Camellia sinensis (L.) O. Kuntze) as a potential source of total phenolics and antioxidant activity. In Vitro Cell Dev Biol Plant 49:60–69

    Article  Google Scholar 

  • Hazarika R, Mishra V K, Chaturvedi R (2013) In Vitro Haploid Production—A Fast and Reliable Approach for Crop Improvement. In: Tuteja N, Gill S (eds) Crop improvement under adverse conditions. Springer, New York, pp 171–212

    Google Scholar 

  • Höfer M (2004) In vitro androgenesis in apple—improvement of the induction phase. Plant Cell Rep 22:365–370

    Article  CAS  PubMed  Google Scholar 

  • Hu T, Kasha KJ (1997) Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.). Can J Plant Sci 77:549–554

    Article  Google Scholar 

  • Islam SS, Tuteja N (2012) Enhancement of androgenesis by abiotic stress and other pretreatments in major crop species. Plant Sci 182:134–144

    Article  CAS  PubMed  Google Scholar 

  • Jacquard C, Asakaviciute R, Hamalian AM, Sangwan RS, Devaux P, Clement C (2006) Barley anther culture: effects of annual cycle and spike position on microspore embryogenesis and albinism. Plant Cell Rep 25:375–381

    Article  CAS  PubMed  Google Scholar 

  • Karasawa MMG, Chiancone B, Gianguzzi V, Abdelgalel AM, Botta R, Sartor C, Germanà MA (2016) Gametic embryogenesis through isolated microspore culture in Corylus avellana L. Plant Cell Tissue Organ Cult 124:635–647

    Article  Google Scholar 

  • Kasha KJ, Kao KN (1970) High frequency haploid production in barley (Hordeum vulgare L.). Natur 225:874–876

    Article  CAS  Google Scholar 

  • Kasha KJ (1974) Haploids in higher plants: advances and potential. In: Proceedings of the 1st international symposium, University of Guelph, Guelph

    Google Scholar 

  • Katiyar S, Mukhtar HA (1996) Tea in chemoprevention of cancer. Int J Oncol 8:221–238

    PubMed  CAS  Google Scholar 

  • Keller WA, Armstrong KC (1979) Stimulation of embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatments. Theor Appl Genet 55:65–67

    Article  CAS  PubMed  Google Scholar 

  • Khush GS, Virmani SS (1996) Haploids in plant breeding. In: Jain SM, Sopory SK, Veilleux R (eds) In Vitro haploid production in higher plants. Springer, Netherlands, pp 11–33

    Chapter  Google Scholar 

  • Larsen ET, Tuvesson IK, Andersen SB (1991) Nuclear genes affecting percentage of green plants in barley (Hordeum vulgare L.) anther culture. Theor Appl Genet 82:417–420

    Article  CAS  PubMed  Google Scholar 

  • Laurie DA, Bennett MD (1988) The production of haploid wheat plants from wheat x maize crosses. Theor Appl Genet 76:393–397

    Article  CAS  PubMed  Google Scholar 

  • Maluszynska J (2003) Cytogenetic tests for ploidy level analyses—chromosome counting. In: Maluszynski M, Kasha K.J, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Springer, Dordrecht, pp 391–395

    Chapter  Google Scholar 

  • Magoon ML, Khanna KR (1963) Haploids Caryologia 16:191–235

    Article  Google Scholar 

  • Maheshwari SC, Rashid A, Tyagi AK (1982) Haploids from pollen grains–retrospect and prospect. Am J Bot 69:865–879

    Article  Google Scholar 

  • Maluszynski M, Kasha KJ, Szarejko I (2003) Published doubled haploid protocols in plant species. Doubled haploid production in crop plants. Springer, Netherlands, pp 309–335

    Chapter  Google Scholar 

  • Mishra VK, Bajpai R, Chaturvedi R (2017) An efficient and reproducible method for development of androgenic haploid plants from in vitro anther cultures of Camellia assamica ssp. assamica (Masters). In Vitro Cell Dev Biol Plant 53:239–248

    Article  CAS  Google Scholar 

  • Mishra VK, Goswami R (2014) Haploid production in higher plant. Int J Chem Biol Sci 1:26–45

    Google Scholar 

  • Mondal BA, Laxmikumaran M, Ahuja PS (2004) Recent advances of tea (Camellia sinensis) biotechnology. Plant Cell Tissue Organ Cult 76:195–254

    Article  CAS  Google Scholar 

  • Murashiage T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15:473–497

    Article  Google Scholar 

  • Nair S, Gupta PK, Mascarenhas AF (1983) Haploid plants from in vitro anther culture of Annona squamosa Linn. Plant Cell Rep 2:198–200

    Article  CAS  PubMed  Google Scholar 

  • Nakajima I, Kobayashi S, Nakamura Y (2000) Embryogenic callus induction and plant regeneration from unfertilized ovule of ‘Kyoho’grape. J Jpn Soc Hortic Sci 69:186–188

    Article  CAS  Google Scholar 

  • Namita P, Mukesh R, Vijay KJ (2012) Camellia sinensis (green tea): a review. Global J Pharmacol 6:52–59

    Google Scholar 

  • Ochatt S, Pech C, Grewal R, Conreux C, Lulsdorf M, Jacas L (2009) Abiotic stress enhances androgenesis from isolated microspores of some legume species (Fabaceae). Plant Physiol 166:1314–1328

    Article  CAS  Google Scholar 

  • Olmedilla A (2010) Microspore embryogenesis In: Plant developmental biology-biotechnological perspectives. Springer, Berlin Heidelberg, pp 27–44

    Chapter  Google Scholar 

  • Palmer CD, Keller WA (2005) Overview of haploidy. In: Haploids in crop improvement II. Springer, Berlin Heidelberg, pp 3–9

    Google Scholar 

  • San Noeum LH (1976) Haploïdes d’ Hordeum vulgare L. par culture in vitro d’ovaires non fécondés. Ann Amelior Plant 26:751–754

    Google Scholar 

  • Scott P, Lyne RL (1994) The effect of different carbohydrate sources upon the initiation of embryogenesis from barley microspores. Plant Cell Tissue Organ Cult 36:129–133

    Article  CAS  Google Scholar 

  • Scott P, Lyne RL, Rees T (1995) Metabolism of maltose and sucrose by microspores isolated from barley (Hordeum vulgare L). Planta 197:435–441

    Article  CAS  Google Scholar 

  • Seguí-Simarro JM (2010) Androgenesis revisited. Bot Rev 76:377–404

    Article  Google Scholar 

  • Seran TH (2007) Anther culture in tea improvement. Med Aromat Plant Sci Biotechnol 1:234–239

    Google Scholar 

  • Shen Y, Pan G, Lübberstedt T (2015) Haploid strategies for functional validation of plant genes. Trends Biotechnol 33:611–620

    Article  CAS  PubMed  Google Scholar 

  • Sita GL (1996) Gynogenic haploids in vitro. In: In vitro haploid production in higher plants. Springer, Netherlands, pp 175–193

    Chapter  Google Scholar 

  • Sopory SK, Maheswari SC (1976) Development of Pollen Embryoids in Anther Cultures of Datura innoxia: I. General observations and effects of physical factors. J Exp Bot 27:49–57

    Article  Google Scholar 

  • Soriano M, Li H, Boutilier K (2013) Microspore embryogenesis: establishment of embryo identity and pattern in culture. Plant Reprod 206:181–196

    Article  Google Scholar 

  • Srivastava P, Chaturvedi R (2008) In vitro androgenesis in tree species: an update and prospect for further research. Biotechnol Adv 26:482–491

    Article  CAS  PubMed  Google Scholar 

  • Srivastava P, Chaturvedi R (2011) Increased production of azadirachtin from an improved method of androgenic cultures of a medicinal tree Azadirachta indica A. Juss Plant Signal Behav 6:974–981

    Article  CAS  PubMed  Google Scholar 

  • Thomas WTB, Newton AC, Wilson A, Booth A, Macaulay M, Keith R (2000) Development of recombinant chromosome substitution lines-a barley resource. SCRI annual report, pp 99–100

    Google Scholar 

  • Touraev A, Ilham A, Vicente O, Heberle-Bors E (1996) Stress-induced microspore embryogenesis in tobacco: an optimized system for molecular studies. Plant Cell Rep 15:561–565

    Article  CAS  PubMed  Google Scholar 

  • Touraev A, Vicente O, Heberle-Bors E (1997) Initiation of microspore embryogenesis by stress. Trends Plant Sci 2:297–302

    Article  Google Scholar 

  • Touraev A, Forster BP, Jain SM (2009) (eds) Advances in haploid production in higher plants. Berlin, Springer, pp 1–208

    Google Scholar 

  • Xu L, Najeeb U, Tang GX, Gu HH, Zhang GQ, He Y, Zhou WJ (2007) Haploid and doubled haploid technology. Adv Bot Res 45:181–216

    Article  CAS  Google Scholar 

  • Zenkteler M, Nitzsche W (1984) Wide hybridization experiments in cereals. Theor Appl Genet 68:311–315

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India

    R. Bajpai & R. Chaturvedi

Authors
  1. R. Bajpai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Chaturvedi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. Chaturvedi .

Editor information

Editors and Affiliations

  1. Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland

    Shri Mohan Jain

  2. Federal Way, Washington, USA

    Pramod Gupta

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer International Publishing AG, part of Springer Nature

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Bajpai, R., Chaturvedi, R. (2018). Haploid Embryogenesis in Tea. In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 85. Springer, Cham. https://doi.org/10.1007/978-3-319-79087-9_26

Download citation

Publish with us

Policies and ethics

Search

Navigation