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Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants pp 231–244Cite as

Somatic Embryogenesis of Date Palm (Phoenix dactylifera L.) from Shoot Tip Explants

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Part of the book series: Forestry Sciences ((FOSC,volume 85))

Abstract

Date palm (Phoenix dactylifera L. ) is a fruit tree adapted the desert climate. The dates are rich in sugar, minerals, vitamins, and numerous pharmaceutical compounds. Seeds propagating results in heterogeneous offsprings with inferior dates. Therefore propagation of offshoots is the preferred method among farmers. This method, however, is constrained by the limited number of offshoots. Currently, micropropagation is used effectively for large-scale commercial propagation of date palm. This chapter describes a micropropagation protocol based on indirect somatic embryogenesis using apical shoot tip explants. Fully-detailed step-wise procedures are described including explant isolation and disinfection, callus induction and maintenance, development of somatic embryos, rooting and plantlet formation, and finally acclimatization and field establishment.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Hassa, 31982, Saudi Arabia

    Jameel M. Al-Khayri

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  1. Jameel M. Al-Khayri
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Correspondence to Jameel M. Al-Khayri .

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Editors and Affiliations

  1. Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland

    Shri Mohan Jain

  2. Federal Way, Washington, USA

    Pramod Gupta

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Al-Khayri, J.M. (2018). Somatic Embryogenesis of Date Palm (Phoenix dactylifera L.) from Shoot Tip Explants. In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 85. Springer, Cham. https://doi.org/10.1007/978-3-319-79087-9_19

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