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Affinity Membranes for Capture of Cells and Biological Substances

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Filtering Media by Electrospinning

Abstract

Fundamental approaches of developing electrospun affinity membranes and their applications in cell capture and separation of biologically active substances are discussed. Basic principles of affinity membrane-based separation process and types of various surface modifications are described, to produce electrospun affinity nanofibers for filtration or fractionation of desired biological substances. The relevant advancements in electrospinning for novel surface modifications have been discussed with a special focus on coupled ligands and molecular imprinted polymers. Applications pertaining to cell capturing and filtration of biomolecules are reviewed in detail, with reference to recent reports on electrospun affinity nanofiber membranes for various antibody and protein purification.

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Authors and Affiliations

  1. AMBER, CRANN Institute, Trinity College Dublin, Dublin, Republic of Ireland

    Rameshkumar Saranya, Rajendiran Murugan, Manasa Hegde, James Doyle & Ramesh Babu

  2. School of Physics, Trinity College Dublin, Dublin, Republic of Ireland

    Rameshkumar Saranya, Manasa Hegde & Ramesh Babu

  3. OEM Diagnostics, Merck Ltd, County Cork, Republic of Ireland

    Rajendiran Murugan

  4. BEACON - Bioeconomy Research Centre, University College Dublin, Dublin, Republic of Ireland

    Ramesh Babu

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  1. Rameshkumar Saranya
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  2. Rajendiran Murugan
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  3. Manasa Hegde
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  4. James Doyle
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Correspondence to Ramesh Babu .

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Editors and Affiliations

  1. Department of Chemistry “Ciamician”, University of Bologna, Bologna, Italy

    Maria Letizia Focarete

  2. Department of Chemistry “Ciamician”, University of Bologna, Bologna, Italy

    Chiara Gualandi

  3. National University of Singapore, Singapore, Singapore

    Seeram Ramakrishna

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Saranya, R., Murugan, R., Hegde, M., Doyle, J., Babu, R. (2018). Affinity Membranes for Capture of Cells and Biological Substances. In: Focarete, M., Gualandi, C., Ramakrishna, S. (eds) Filtering Media by Electrospinning. Springer, Cham. https://doi.org/10.1007/978-3-319-78163-1_8

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