Abstract
Ross Harrison (1870–1959) was the first to attempt to culture tissues in vitro using culture dishes. In 1907 he had grown tadpole tissue in a coagulated interstitial liquid from frog using the hanging drop technique [104]. This technique is still used today to generate spheroids in vitro and to culture them.
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References
Harrison R (1907) Observations on the living developing nerve fiber. Anat Rec, Proc Soc Exp Med, NY 1:116–128
Carrel A (1912) Pure cultures of cells. J Exp Med 16(2):165–168
Carrel A (1912) On the permanent life of tissues outside of the organism. J Exp Med 15(5):516–528
Eagle H (1955) Nutrition needs of mammalian cells in tissue culture. Science 122(3168):501–514
Jaffe EA, Nachman RL, Becker CG, Minick CR (1973) Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 52(11):2745–2756. https://doi.org/10.1172/JCI107470
Gimbrone MA Jr, Cotran RS, Folkman J (1973) Endothelial regeneration: studies with human endothelial cells in culture. Ser Haematol 6(4):453–455
Campbell GR, Uehara Y, Mark G, Burnstock G (1971) Fine structure of smooth muscle cells grown in tissue culture. J Cell Biol 49(1):21–34
Chamley-Campbell J, Campbell GR, Ross R (1979) The smooth muscle cell in culture. Physiol Rev 59(1):1–61
Buzney SM, Frank RN, Robison WG Jr (1975) Retinal capillaries: proliferation of mural cells in vitro. Science 190(4218):985–986
Weibel ER, Palade GE (1964) New cytoplasmic components in arterial endothelia. J Cell Biol 23:101–112
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Bikfalvi, A. (2017). Culture of Vascular Cells In Vitro. In: A Brief History of Blood and Lymphatic Vessels. Springer, Cham. https://doi.org/10.1007/978-3-319-74376-9_5
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DOI: https://doi.org/10.1007/978-3-319-74376-9_5
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