Abstract
It is important to have methods and models that allow the study of angiogenesis. Obviously, the field of angiogenesis uses all the experimental methods of cellular, molecular, and developmental biology. Nevertheless, angiogenesis research needs some very specific methods and models that require precise technical knowledge. These can be classified into three categories: in vitro models, ex vivo models, and in vivo models.
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le Noble F, Moyon D, Pardanaud L, Yuan L, Djonov V, Matthijsen R, Breant C, Fleury V, Eichmann A (2004) Flow regulates arterial-venous differentiation in the chick embryo yolk sac. Development 131(2):361–375. https://doi.org/10.1242/dev.00929
Boye K, Pujol N, D Alves I, Chen YP, Daubon T, Lee YZ, Dedieu S, Constantin M, Bello L, Rossi M, Bjerkvig R, Sue SC, Bikfalvi A, Billottet C (2017) The role of CXCR3/LRP1 cross-talk in the invasion of primary brain tumors. Nat Commun 8(1):1571. https://doi.org/10.1038/s41467-017-01686-y
Hagedorn M, Javerzat S, Gilges D, Meyre A, de Lafarge B, Eichmann A, Bikfalvi A (2005) Accessing key steps of human tumor progression in vivo by using an avian embryo model. Proc Natl Acad Sci U S A 102(5):1643–1648. https://doi.org/10.1073/pnas.0408622102
Dumartin L, Quemener C, Laklai H, Herbert J, Bicknell R, Bousquet C, Pyronnet S, Castronovo V, Schilling MK, Bikfalvi A, Hagedorn M (2010) Netrin-1 mediates early events in pancreatic adenocarcinoma progression, acting on tumor and endothelial cells. Gastroenterology 138(4):1595–1606, 1606 e1591–1598. https://doi.org/10.1053/j.gastro.2009.12.061
Hagedorn M, Balke M, Schmidt A, Bloch W, Kurz H, Javerzat S, Rousseau B, Wilting J, Bikfalvi A (2004) VEGF coordinates interaction of pericytes and endothelial cells during vasculogenesis and experimental angiogenesis. Dev Dyn 230(1):23–33. https://doi.org/10.1002/dvdy.20020
Soulet F, Kilarski WW, Roux-Dalvai F, Herbert JM, Sacewicz I, Mouton-Barbosa E, Bicknell R, Lalor P, Monsarrat B, Bikfalvi A (2013) Mapping the extracellular and membrane proteome associated with the vasculature and the stroma in the embryo. Mol Cell Proteomics 12(8):2293–2312. https://doi.org/10.1074/mcp.M112.024075
Saidi A, Javerzat S, Bellahcene A, De Vos J, Bello L, Castronovo V, Deprez M, Loiseau H, Bikfalvi A, Hagedorn M (2008) Experimental anti-angiogenesis causes upregulation of genes associated with poor survival in glioblastoma. Int J Cancer/Journal international du cancer 122(10):2187–2198. https://doi.org/10.1002/ijc.23313
Javerzat S, Franco M, Herbert J, Platonova N, Peille AL, Pantesco V, De Vos J, Assou S, Bicknell R, Bikfalvi A, Hagedorn M (2009) Correlating global gene regulation to angiogenesis in the developing chick extra-embryonic vascular system. PLoS One 4(11):e7856. https://doi.org/10.1371/journal.pone.0007856
Cavill R, Sidhu JK, Kilarski W, Javerzat S, Hagedorn M, Ebbels TM, Bikfalvi A, Keun HC (2010) A combined metabonomic and transcriptomic approach to investigate metabolism during development in the chick chorioallantoic membrane. J Proteome Res 9(6):3126–3134. https://doi.org/10.1021/pr100033t
Quemener C, Baud J, Boye K, Dubrac A, Billottet C, Soulet F, Darlot F, Dumartin L, Sire M, Grepin R, Daubon T, Rayne F, Wodrich H, Couvelard A, Pineau R, Schilling M, Castronovo V, Sue SC, Clarke K, Lomri A, Khatib AM, Hagedorn M, Prats H, Bikfalvi A (2016) Dual roles for CXCL4 chemokines and CXCR3 in angiogenesis and invasion of pancreatic cancer. Cancer Res 76(22):6507–6519. https://doi.org/10.1158/0008-5472.CAN-15-2864
Russell WMS, Burch RL (1959) The principles of humane experimental technique. Methuen, London
Thomas JL, Baker K, Han J, Calvo C, Nurmi H, Eichmann AC, Alitalo K (2013) Interactions between VEGFR and Notch signaling pathways in endothelial and neural cells. Cell Mol Life Sci 70(10):1779–1792. https://doi.org/10.1007/s00018-013-1312-6
Folkman J, Watson K, Ingber D, Hanahan D (1989) Induction of angiogenesis during the transition from hyperplasia to neoplasia. Nature 339(6219):58–61. https://doi.org/10.1038/339058a0
Casanovas O, Hicklin DJ, Bergers G, Hanahan D (2005) Drug resistance by evasion of antiangiogenic targeting of VEGF signaling in late-stage pancreatic islet tumors. Cancer Cell 8(4):299–309. https://doi.org/10.1016/j.ccr.2005.09.005
Lu KV, Chang JP, Parachoniak CA, Pandika MM, Aghi MK, Meyronet D, Isachenko N, Fouse SD, Phillips JJ, Cheresh DA, Park M, Bergers G (2012) VEGF inhibits tumor cell invasion and mesenchymal transition through a MET/VEGFR2 complex. Cancer Cell 22(1):21–35. https://doi.org/10.1016/j.ccr.2012.05.037
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Bikfalvi, A. (2017). How to Study Angiogenesis?. In: A Brief History of Blood and Lymphatic Vessels. Springer, Cham. https://doi.org/10.1007/978-3-319-74376-9_11
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DOI: https://doi.org/10.1007/978-3-319-74376-9_11
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