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Cardiac Regeneration pp 145–161Cite as

Application of the Suspension Culture System for Scale-Up Manufacture of hPSCs and hPSC-Derived Cardiomyocytes

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Part of the book series: Cardiac and Vascular Biology ((Abbreviated title: Card. vasc. biol.,volume 4))

Abstract

Establishment of a scalable, robust, and GMP-compatible manufacturing process for human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) has been a bottleneck for the progress of cell therapy for heart diseases. The adherent cell culture platforms have been well developed for hPSC maintenance and cardiac differentiation. However, the two-dimensional culture system is limited by its scalability, hindering its application for scale-up cell production. Recent advances in development of suspension culture systems, which provide the advantage of scalability, have driven hPSC-CMs beyond bench research into preclinical development. With the suspension platform, the processes from hPSC expansion to cardiac differentiation have been streamlined for the hPSC-CM production. A fully suspension-based process avoids extensive labor associated with the conventional adherent culture and lowers production costs by reducing reagents, space, and operators. These advantages render this manufacturing process more manageable, cost-effective, labor-effective, and practical for large-scale cell production. In this chapter, we will review current status of the development of the suspension culture system for hPSC-CM production.

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Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.

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Authors and Affiliations

  1. Center for Biomedicine and Genetics, Beckman Research Institute of City of Hope, 1500 E. Duarte Road, Duarte, CA, 91010, USA

    Vincent C. Chen & Joseph Gold

  2. Arrogene, 8560 W. Sunset Blvd. Suite 424, Los Angeles, CA, 90069, USA

    Larry A. Couture

  3. Orbsen Therapeutics, Orbsen Building, NUIG, University Road Galway, Ireland, Ireland

    Larry A. Couture

Authors
  1. Vincent C. Chen
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  2. Larry A. Couture
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  3. Joseph Gold
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Correspondence to Vincent C. Chen .

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Editors and Affiliations

  1. Keio University, School of Medicine, Department of Cardiology, Tokyo, Japan

    Masaki Ieda

  2. Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Georg-August University, Göttingen, Germany

    Wolfram-Hubertus Zimmermann

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Chen, V.C., Couture, L.A., Gold, J. (2017). Application of the Suspension Culture System for Scale-Up Manufacture of hPSCs and hPSC-Derived Cardiomyocytes. In: Ieda, M., Zimmermann, WH. (eds) Cardiac Regeneration. Cardiac and Vascular Biology, vol 4. Springer, Cham. https://doi.org/10.1007/978-3-319-56106-6_7

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