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Generation and Application of Human Pluripotent Stem Cell-Derived Cardiomyocytes

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Cardiac Regeneration

Part of the book series: Cardiac and Vascular Biology ((Abbreviated title: Card. vasc. biol.,volume 4))

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Abstract

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are a powerful technology with established applications in regenerative medicine, disease modeling, drug testing and discovery, and developmental biology. The in vitro generation of human cardiomyocytes has progressed rapidly over the last 15 years, and cardiac differentiation of human pluripotent stem cells (hPSC) is now a simple and routine process for many laboratories, generating contracting fetal-like cardiomyocytes that recapitulate a variety of known cardiovascular disease phenotypes. Here we discuss the history and existing knowledge in the field on the generation of cardiomyocytes from human pluripotent stem cells. We cover the potential clinical and investigational applications of hPSC-CMs, as well as the role of the pluripotent state in differentiation, and current methodologies for differentiation, purification, directed cardiomyocyte subtype specification and maturation, and large-scale production. We conclude with a discussion on the future of the field.

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Acknowledgments

We would like to acknowledge funding support from the US National Institutes of Health R00 HL121177, the Dixon Translational Research Grant Young Investigator Award, and Innovation Development Progress (IDP) Research Innovation Challenge Grant (P.W.B.). We apologize to those investigators whose work was omitted here due to space limitations.

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Conflict of Interest

P.W.B. is on the scientific advisory board of Pluriomics B.V. and a shareholder in Stem Cell Theranostics.

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This article does not contain any studies with human participants performed by any of the authors.

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Authors and Affiliations

  1. Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA

    Adam J. T. Schuldt, Marisol Romero-Tejeda & Paul W. Burridge

  2. Center for Pharmacogenomics, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA

    Adam J. T. Schuldt, Marisol Romero-Tejeda & Paul W. Burridge

  3. Division of Cardiology, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA

    Adam J. T. Schuldt

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  1. Adam J. T. Schuldt
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  2. Marisol Romero-Tejeda
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  3. Paul W. Burridge
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Correspondence to Paul W. Burridge .

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Editors and Affiliations

  1. Keio University, School of Medicine, Department of Cardiology, Tokyo, Japan

    Masaki Ieda

  2. Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Georg-August University, Göttingen, Germany

    Wolfram-Hubertus Zimmermann

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Schuldt, A.J.T., Romero-Tejeda, M., Burridge, P.W. (2017). Generation and Application of Human Pluripotent Stem Cell-Derived Cardiomyocytes. In: Ieda, M., Zimmermann, WH. (eds) Cardiac Regeneration. Cardiac and Vascular Biology, vol 4. Springer, Cham. https://doi.org/10.1007/978-3-319-56106-6_4

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