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Alternatives for Dermal Toxicity Testing pp 331–345Cite as

Human Peripheral Blood Monocyte Derived Dendritic Cells Assay for the Detection and Characterization of Sensitizers

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Abstract

The human peripheral blood monocyte derived dendritic cells (PBMDCs) assay is an optimized test protocol for the detection and characterization of the sensitizing properties of chemicals. Using primary cells of human origin as a test system, it delivers additional and/or confirmatory information relevant to the human in vivo situation when integrated in a test strategy. The reproducibility and predictivity of the PBMDC assay have been evaluated with multiple chemical test sets and during a ring study organized with five laboratories. Additionally, the PBMDC participated in a systematic and comparative evaluation of various in vitro test methods for skin sensitization safety assessment. The assay was shown to be robust and transferable and it delivered results that compared very well with corresponding human sensitization data of chemicals including pro-haptens. It should be regarded as a primary source of information concerning the DC activation properties of the tested molecule or as an addition and/or a confirmation to the results already obtained with a cell line based test system.

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Acknowledgements

We thank Silke Gerlach and Jochem Spieker for excellent technical assistance and valuable intellectual contributions in the course of assay development.

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Authors and Affiliations

  1. Beiersdorf AG, Hamburg, Germany

    Andreas Schepky, Hendrik Reuter & Jochen Kühnl

  2. Independant Consultant, Marly, Switzerland

    Pierre Aeby

Authors
  1. Andreas Schepky
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  2. Hendrik Reuter
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  3. Jochen Kühnl
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  4. Pierre Aeby
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Corresponding author

Correspondence to Andreas Schepky .

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Editors and Affiliations

  1. SeCAM, Magliaso, Switzerland

    Chantra Eskes

  2. Innovitox Consulting & Services, Houten, The Netherlands

    Erwin van Vliet

  3. Department of Dermatology, University of California School of Medicine, San Francisco, California, USA

    Howard I. Maibach

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Schepky, A., Reuter, H., Kühnl, J., Aeby, P. (2017). Human Peripheral Blood Monocyte Derived Dendritic Cells Assay for the Detection and Characterization of Sensitizers. In: Eskes, C., van Vliet, E., Maibach, H. (eds) Alternatives for Dermal Toxicity Testing. Springer, Cham. https://doi.org/10.1007/978-3-319-50353-0_23

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  • DOI: https://doi.org/10.1007/978-3-319-50353-0_23

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  • Online ISBN: 978-3-319-50353-0

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