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Skin Irritation Hazard of Chemicals Assessed by the EpiSkin™ In Vitro Test Method

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Abstract

The EpiSkin™ skin irritation method is an internationally accepted test method used to detect the skin irritation properties of chemicals. The EpiSkin™ skin irritation test method has been shown to be robust and transferable. The reproducibility and predictivity of the EpiSkin™ assay have been evaluated in validation studies organized with three laboratories, and with a large set of 122 chemicals through a systematic in vivo Draize comparative evaluation for safety assessment, that delivered results that matched very well with corresponding in vivo data showing 75% for specificity (n = 65), 94% for sensitivity (n = 57) and 84% for accuracy (n = 122). It could be regarded as a primary source of information concerning the skin irritation properties of the tested chemical and be used in combination with a battery of assays, such as the EpiSkin™ skin corrosion method, in any initiative aimed at developing integrated testing strategies for full evaluation of local skin effects.

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Authors and Affiliations

  1. L’Oréal Research and Innovation, 1, Avenue Eugène Schueller, 93600, Aulnay-sous-Bois Cedex, France

    Nathalie Alépée, Marie Hélène Grandidier & José Cotovio

Authors
  1. Nathalie Alépée
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  2. Marie Hélène Grandidier
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  3. José Cotovio
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Correspondence to Nathalie Alépée .

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Editors and Affiliations

  1. SeCAM, Magliaso, Switzerland

    Chantra Eskes

  2. Innovitox Consulting & Services, Houten, The Netherlands

    Erwin van Vliet

  3. Department of Dermatology, University of California School of Medicine, San Francisco, California, USA

    Howard I. Maibach

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Alépée, N., Grandidier, M.H., Cotovio, J. (2017). Skin Irritation Hazard of Chemicals Assessed by the EpiSkin™ In Vitro Test Method. In: Eskes, C., van Vliet, E., Maibach, H. (eds) Alternatives for Dermal Toxicity Testing. Springer, Cham. https://doi.org/10.1007/978-3-319-50353-0_2

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  • DOI: https://doi.org/10.1007/978-3-319-50353-0_2

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