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A Laboratory Guide to Glycoconjugate Analysis pp 23–39Cite as

Detection of the Presence of Carbohydrates in Glycoproteins

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Part of the book series: BioMethods ((BIOMETHODS))

Abstract

It is now recognized that many proteins are in fact glycosylated, (1) and that the attached carbohydrate chains or glycans can have significant effects on the physical properties and biological activity of the protein (2, 3). The detection of carbohydrates in glycoconjugates, and in particular in glycoproteins, is therefore a subject which is increasingly being addressed by researchers.

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References

  1. Montreuil J (1982). In: G. Florkin (ed.) Comprehensive Biochemistry, Vol. 19, part 2, p. 1, Elsevier, Amsterdam.

    Google Scholar 

  2. Varki A (1993) Glycobiology 3: 97.

    Article  PubMed  CAS  Google Scholar 

  3. Kobata A (1992) Structures and functions of the sugar chains of glycoproteins. Eur. J. Biochem. 209: 483.

    Article  PubMed  CAS  Google Scholar 

  4. Dwek RA, Edge CJ, Harvey DJ, Wormald MR and Parekh RB (1993) Glycoform analysis of glycoproteins. Ann. Rev. Biochem. 62: 6.

    Article  Google Scholar 

  5. Kobata A (1992) Structures and functions of the sugar chains of glycoproteins. Eur. J. Biochem. 209: 483.

    Article  PubMed  CAS  Google Scholar 

  6. Zacharius, RM (1969) Anal. Biochem. 30: 148.

    Article  PubMed  CAS  Google Scholar 

  7. Bayer EA, Ben-Hur H and Wilchek M (1990) Methods Enzymol. 184: 415.

    Article  PubMed  CAS  Google Scholar 

  8. Durrant I, Benge LC, Sturrock C, Devenish AT, Howe R, Roe S, Moore M, Scozzafava G, Proudfoot LM and Richardson TC, (1990) Biotechniques 8: 564.

    PubMed  CAS  Google Scholar 

  9. Haltiwagner RS, Kelly WG, Blomberg MA, Dong D, Roquermone E and Hart GW (1992) Biochem. Soc. Trans. 20, 264.

    Google Scholar 

  10. Laemmli UK (1970) Nature 277: 680.

    Article  Google Scholar 

  11. Hames BD (1981) In: B.D. Hannes and D. Rickwood (eds) An Introduction to Polyacylamide Gel Electrophoresis: A Practical Approach, IRL, Oxford.

    Google Scholar 

  12. Towbin H, Towbin R, Dunbar JS, and Bone K (1979) Proc. Natl. Acad. Sci. USA 76: 4350.

    Article  PubMed  CAS  Google Scholar 

  13. Okamura H, Sigal CT, Alland L, Resh MD (1995) Methods Enzymol. 254: 535.

    Article  PubMed  CAS  Google Scholar 

  14. Gravel P, Golaz O, Walzer C, Hochstrasse DF, Turler H and Balant LP (1994) Anal. Biochem. 221: 66.

    Article  PubMed  CAS  Google Scholar 

  15. Brummell DA, Sharma VP, Anand NN, Bilous D, Dubuc G, Michniewicz J, Mackenzie CR, Sadowska J, Sigurkjold BW, Sinnat B, Young M, Bundle DR and Narang SA(1993) Biochemistry 32: 1180.

    Google Scholar 

  16. Whiteheart SW, Passaniti A, Reichner JS, Holt GD, Haltiwanger RS, and Hart GW (1989) Methods Enzymol. 179: 82.

    Article  PubMed  CAS  Google Scholar 

  17. Brossmer R and Gross HJ (1994) Methods Enzymol. 247: 177.

    Article  PubMed  CAS  Google Scholar 

  18. Ferguson MAJ and Williams A F (1988) Ann. Rev. Biochem. 57, 285.

    Article  PubMed  CAS  Google Scholar 

  19. Bordier CJ (1981) Biol. Chem. 256: 1604.

    CAS  Google Scholar 

  20. Kelly WG and Hart GW (1989) Cell 57: 243.

    Article  PubMed  CAS  Google Scholar 

  21. Kearse KP and Hart GW (1991) Proc. Natl. Acad. Sci. USA 88: 1701.

    Article  CAS  Google Scholar 

  22. Holt GD, Haltiwanger RS, Torres CR and Hart GW (1987) J. Biol. Chem. 262: 14847.

    PubMed  CAS  Google Scholar 

  23. Alexander et al (1989) Methods Enzymol. 179: 505.

    Google Scholar 

  24. Glasgow LR and LueckeRH (1977) J. Biol. Chem. 252: 8615.

    Google Scholar 

  25. Patel T, Bruce J, Merry A, Bigge C, Wormald M, Jaques A, Parekh R, (1993)

    Google Scholar 

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Authors
  1. Tony H. Merry
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  2. Mary Steventon
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Editor information

Editors and Affiliations

  1. Division of Transfusion Medicine, University of Cambridge East Anglian Blood Centre, Long Road, CB2 2PT, Cambridge, UK

    Peter Jackson

  2. Cancer Research Campaign Department of Medical Oncology, University of Manchester Christie CRC Research Centre, Wilmslow Road, M20 4BX, Manchester, UK

    John T. Gallagher

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© 1997 Birkhäuser Verlag Basel

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Merry, T.H., Steventon, M. (1997). Detection of the Presence of Carbohydrates in Glycoproteins. In: Jackson, P., Gallagher, J.T. (eds) A Laboratory Guide to Glycoconjugate Analysis. BioMethods. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7388-8_2

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  • DOI: https://doi.org/10.1007/978-3-0348-7388-8_2

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7390-1

  • Online ISBN: 978-3-0348-7388-8

  • eBook Packages: Springer Book Archive

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