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International Conference on Bio and Nanomaterials

BIONAM 2019 2019: Advances in Bionanomaterials II pp 223–232Cite as

Single Compartment Approach for Assembling Photosynthetic Protocells

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Part of the book series: Lecture Notes in Bioengineering ((LNBE))

Abstract

In this work, we show the extraction, purification and reconstitution of the bacterial protein complex bc1 in giant lipid vesicles used as versatile micro-sized enzymatic reactors. Firstly, it is demonstrated that the bc1 shows an enzymatic activity also as micellar suspension when the proper substrates are added. Moreover, it has been possible to reconstitute, with the droplet transfer method, the bc1 complex in artificial giant lipid vesicles. The reconstitution has been proved by confocal microscopy analysis demonstrating a change of internal pH, thanks to a pH-sensitive fluorescent dye, upon the addition of a trigger substrate.

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of Bari Aldo Moro, Bari, Italy

    Emiliano Altamura, Paola Albanese & Fabio Mavelli

  2. CNR-ISPA, Institute of Sciences of Food Production, Lecce Unit, Lecce, Italy

    Francesco Milano

  3. CNR-IPCF, Institute for Physical and Chemical Processes, Bari, Italy

    Massimo Trotta

  4. Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy

    Pasquale Stano

  5. Institute of Nanotechnology, CNR NANOTEC, Bari, Italy

    Fabio Mavelli

Authors
  1. Emiliano Altamura
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  2. Paola Albanese
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  3. Francesco Milano
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  4. Massimo Trotta
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  5. Pasquale Stano
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  6. Fabio Mavelli
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Corresponding author

Correspondence to Fabio Mavelli .

Editor information

Editors and Affiliations

  1. Department of Pharmacy, University of Salerno, Fisciano, Italy

    Stefano Piotto

  2. Department of Industrial Engineering, University of Salerno, Fisciano, Italy

    Simona Concilio

  3. Department of Pharmacy, University of Salerno, Fisciano, Italy

    Lucia Sessa

  4. Department of Earth, Environmental and Physical Sciences, University of Siena, Siena, Italy

    Federico Rossi

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Altamura, E., Albanese, P., Milano, F., Trotta, M., Stano, P., Mavelli, F. (2020). Single Compartment Approach for Assembling Photosynthetic Protocells. In: Piotto, S., Concilio, S., Sessa, L., Rossi, F. (eds) Advances in Bionanomaterials II. BIONAM 2019 2019. Lecture Notes in Bioengineering. Springer, Cham. https://doi.org/10.1007/978-3-030-47705-9_19

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  • DOI: https://doi.org/10.1007/978-3-030-47705-9_19

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-47704-2

  • Online ISBN: 978-3-030-47705-9

  • eBook Packages: EngineeringEngineering (R0)

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