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Coconut Biotechnology: Towards the Sustainability of the ‘Tree of Life’ pp 199–225Cite as

Germplasm Reestablishment and Seedling Production: Embryo Culture

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Abstract

This chapter discusses coconut embryo culture (EC) including the morphology and physiology of the zygotic coconut embryo, culture types and growth conditions, applications such as the use of the EC technique for the collection of germplasm from isolated/distant areas, conservation (cryopreservation) and the dissemination of elite germplasm, and as a starting point for clonal propagation (via somatic embryogenesis). The technique is most commonly recognized for its application to coconut types that generally don’t germinate in vivo. As EC is the foundation tissue culture method for cloning, propagation, conservation, and transformation of coconut, the technique has been widely documented. However, the method is generally underused, and protocols have room for improvement. Key enhancements needed are method optimizations to ensure high survival rates for a wide range of genotypes, in a variety of laboratories. Losses can be high during acclimatization and transfer to field conditions.

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Acknowledgments

The authors greatly appreciate the support from Kokonas Indastri Koporesen (KIK) and the Australian Centre for International Agricultural Research (ACIAR).

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Authors and Affiliations

  1. School of Agriculture and Food Science, University of Queensland, St Lucia, QLD, Australia

    Julianne Biddle, Quang Nguyen, Zhihua H. Mu, Mike Foale & Steve Adkins

  2. Applied Biotechnology in Crop Development Research Unit, School of Biotechnology, International University, Vietnam National University-HCM, Ho Chi Minh City, Vietnam

    Quang Nguyen

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  1. Julianne Biddle
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  2. Quang Nguyen
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Correspondence to Julianne Biddle .

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  1. School of Agriculture and Food Sciences, The University of Queensland, St Lucia, QLD, Australia

    Steve Adkins

  2. School of Agriculture and Food Sciences, The University of Queensland, St Lucia, QLD, Australia

    Mike Foale

  3. Biological Systems Department, Centre de coopération internationale en recherche agronomique pour le développement (CIRAD), Montpellier, QLD, Australia

    Roland Bourdeix

  4. School of Agriculture and Food Sciences, The University of Queensland, St Lucia, QLD, Australia

    Quang Nguyen

  5. School of Agriculture and Food Science, University of Queensland, St. Lucia, QLD, Australia

    Julianne Biddle

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Biddle, J., Nguyen, Q., Mu, Z.H., Foale, M., Adkins, S. (2020). Germplasm Reestablishment and Seedling Production: Embryo Culture. In: Adkins, S., Foale, M., Bourdeix, R., Nguyen, Q., Biddle, J. (eds) Coconut Biotechnology: Towards the Sustainability of the ‘Tree of Life’. Springer, Cham. https://doi.org/10.1007/978-3-030-44988-9_10

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