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Molecular Imaging in Oncology pp 533–561Cite as

Molecular Imaging in Oncology: Advanced Microscopy Techniques

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Part of the book series: Recent Results in Cancer Research ((RECENTCANCER,volume 216))

Abstract

Preclinical studies usually require high levels of morphological, functional, and biochemical information at subcellular resolution. This type of information cannot be obtained from clinical imaging techniques, such as MRI, PET/CT, or US. Luckily, many microscopy techniques exist that can offer this information, also for malignant tissues and therapeutic approaches. In this overview, we discuss the various advanced optical microscopy techniques and their applications in oncological research. After a short introduction in Sect. 16.1, we continue in Sect. 16.2 with a discussion on fluorescent labelling strategies, followed in Sect. 16.3 by an in-depth description of confocal, light-sheet, two-photon, and super-resolution microscopy. We end in Sect. 16.4 with a focus on the applications, specifically in oncology.

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Authors and Affiliations

  1. School for Oncology and Developmental Biology GROW and School for Cardiovascular Diseases CARIM, Maastricht University, Maastricht, The Netherlands

    Dimitrios Kapsokalyvas & Marc A. M. J. van Zandvoort

  2. Institut für Molekulare Kreislaufforschung, Universitätsklinikum Aachen, Aachen, Germany

    Dimitrios Kapsokalyvas & Marc A. M. J. van Zandvoort

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  1. Dimitrios Kapsokalyvas
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  2. Marc A. M. J. van Zandvoort
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Correspondence to Marc A. M. J. van Zandvoort .

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Editors and Affiliations

  1. Department of Nuclear Medicine, University of Münster, Münster, Germany

    Otmar Schober

  2. Institute for Experimental Molecular Imaging, University Hospital, RWTH Aachen University, Aachen, Germany

    Fabian Kiessling

  3. Department of Radiation Oncology, Ruprecht Karl University, Heidelberg, Germany

    Jürgen Debus

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Kapsokalyvas, D., van Zandvoort, M.A.M.J. (2020). Molecular Imaging in Oncology: Advanced Microscopy Techniques. In: Schober, O., Kiessling, F., Debus, J. (eds) Molecular Imaging in Oncology. Recent Results in Cancer Research, vol 216. Springer, Cham. https://doi.org/10.1007/978-3-030-42618-7_16

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