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Tissue Culture-Mediated Biotechnological Advancements in Genus Brassica

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Book cover Brassica Improvement

Abstract

Brassica is documented as a distinguished genus, comprising a wide array of species, belonging to the family Brassicaceae that are majorly consumed as vegetables and processed for oil and condiments. Conventional breeding approaches are laborious; in addition, higher environmental influence interferes with the yield and quality of crop; hence, biotechnological intervention becomes a major requirement. Till date, a lot of studies have been carried out on biotechnological advancements of genus Brassica. Among those, tissue culture-mediated approaches, such as development of protocols for in vitro mass propagation, somaclonal variation, and secondary metabolites production, are highlighted in this chapter. Additionally, genetic transformation and protoplast culture, the other two major tissue culture-based methods, which were substantially used for genetic improvement of Brassica, have also been discussed.

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Abbreviations

2,4-D:

2,4-dichlorophenoxy acetic acid

B5:

Gamborg’s medium

BA:

N6-benzyladenine

BAP:

N6-benzylaminopurine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

Kinetin:

6-furfurylaminopurine

MS:

Murashige and Skoog medium

NAA:

α-Naphthalene acetic acid

PGR:

Plant growth regulator

SE:

Somatic embryo

TDZ:

N-phenyl-N′-(1,2,3-thidiazol-5-yl) urea or Thidiazuron

Zeatin:

4-hydroxy-3-methyl-terms-2-butenyl aminopurine

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Acknowledgements

The authors acknowledge the e-library assistance from the Central Library, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, West Bengal, India. Further, they are thankful to the anonymous reviewers and the editor of this chapter for their critical comments and suggestions on the manuscript.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, India

    Monisha Mitra

  2. Crop Research Unit (Genetics and Plant Breeding), Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, India

    Saikat Gantait

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  1. Monisha Mitra
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  2. Saikat Gantait
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Correspondence to Saikat Gantait .

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Editors and Affiliations

  1. Mountain Research Centre for Field Crops, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India

    Shabir Hussain Wani

  2. ICAR-Directorate of Rapeseed-Mustard Research, Sewar, Bharatpur, Rajasthan, India

    Ajay Kumar Thakur

  3. Division of Genomic Resources, ICARNBPGR, and faculty Bioinformatics, ICAR- IARI and ICAR-IASRI, Pusa Campus, New Delhi, India

    Yasin Jeshima Khan

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Mitra, M., Gantait, S. (2020). Tissue Culture-Mediated Biotechnological Advancements in Genus Brassica. In: Wani, S., Thakur, A., Jeshima Khan, Y. (eds) Brassica Improvement. Springer, Cham. https://doi.org/10.1007/978-3-030-34694-2_5

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