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Recent Advances in Cytoplasmic Male Sterility (CMS) in Crop Brassicas

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Brassica Improvement

Abstract

Brassicas are widely used for different purposes as vegetable oil, condiments, vegetables, biofuels, and animal feed. The cultivated rapeseed-mustard species are adapted in diverse agro-climatic situation ranged from warmer semitropical to cooler temperate regions of the world represented in China, Canada, India, Germany, the UK, France, Australia, Poland, and the USA. Cytoplasmic male sterility (CMS) is one of the extensively studied and useful examples of nucleo-cytoplasmic interactions. CMS is attributed by failure of plant to produce functional/viable pollen grain. CMS is a consequence of conflict/miscoordination between the organelles (mitochondria) and nuclear genes that arises due to instinctively mutation mt DNA and production of alloplasmic substitutions with widely differentiated wild spp. In this chapter, we will discuss the various cytoplasmic male sterility systems with fertility restorer (Rf) genes and advancement in the understanding of fertility restoration mechanism of fertility restorer genes.

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References

  • Atri C, Kaur B, Sharma S et al (2016) Substituting nuclear genome of Brassica juncea (L.) Czern & Coss. In cytoplasmic background of Brassica fruticulosa results in cytoplasmic male sterility. Euphytica 209(1):31–40

    Article  CAS  Google Scholar 

  • Ba Q, Zhang G, Niu N, Ma S, Wang J (2015) Cytoplasmic effects on DNA methylation between male sterile lines and the maintainer in wheat (Triticum aestivum L.). Gene 549:192–197

    Article  CAS  Google Scholar 

  • Banga SS (1993) Heterosis and utilization. In: Labana KS, Banga SS, Banga SK (eds) Breeding oilseed brassicas. Monographs on theoretical and applied genetics, vol 19. Springer, Berlin, pp 21–43

    Chapter  Google Scholar 

  • Banga SS, Banga SK (1997) Enarthrocarpus lyratus cytoplasm causes male sterility in oilseed rape. In: The genetics and exploitation of heterosis in crops. Abs. CIMMYT international symposium, Mexico, 7–22 Aug 1997, pp 120–121

    Google Scholar 

  • Banga S, Kumar PR, Bhajan R, Singh D, Banga SS (2015) Genetics and breeding. In: Kumar A, Banga SS, Meena PD, Kumar PR (eds) Brassica oilseeds: breeding and management. CABI, Wallingford, pp 11–41

    Chapter  Google Scholar 

  • Bannerot H, Boulidard L, Cauderon Y, Temp J. (1974) Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica oleracea. In: Eucarpia Meeting Cruciferae, Dundee, Scotland, pp 52–54

    Google Scholar 

  • Bentolila S, Alfonso AA, Hanson MR (2002) A pentatricopeptide repeat-containing gene restores fertility to cytoplasmic male-sterile plants. Proc Natl Acad Sci U S A 99(16):10887–10892

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bird A (2007) Perceptions of epigenetics. Nature 447:396–398

    Article  CAS  PubMed  Google Scholar 

  • Bisht DS, Chamola R, Nath M, Bhat SR (2015) Molecular mapping of fertility restorer gene of an alloplasmic CMS system in Brassica juncea containing Moricandia arvensis cytoplasm. Mol Breed 35:14

    Article  CAS  Google Scholar 

  • Bohra A, Singh IP, Yadav AK, Pathak A, Soren KR, Chaturvedi SK, Nadarajan N (2015) Utility of informative SSR markers in the molecular characterization of cytoplasmic genetic male sterility-based hybrid and its parents in pigeonpea. Natl Acad Sci Lett 38:13–19

    Article  CAS  Google Scholar 

  • Brown GG, Formanová N, Jin H et al (2003) The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats. Plant J 35:262–272

    Article  CAS  PubMed  Google Scholar 

  • Budar F, Touzet P, De Paepe R (2003) The nucleo-mitochondrial conflict in cytoplasmic male sterilities revisited. Genetica 117:3–16

    Article  CAS  PubMed  Google Scholar 

  • Chase CD (2007) Cytoplasmic male sterility: a window to the world of plant mitochondrial-nuclear interactions. Trends Genet 23:81–90

    Article  CAS  PubMed  Google Scholar 

  • Chen L, Liu YG (2014) Male sterility and fertility restoration in crops. Annu Rev Plant Biol 65:579–606

    Article  CAS  PubMed  Google Scholar 

  • Dahan J, Mireau H (2013) The Rf and Rf-like PPR in higher plants, a fast-evolving subclass of PPR genes. RNA Biol 10(9):1469–1476

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Deol S, Shivanna JR, Prakash K, Banga SS, Robbelen G (2008) Enarthrocarpus lyratus-based cytoplasmic male sterility and fertility restorer system in Brassica rapa. Plant Breed 122:438–440

    Article  Google Scholar 

  • Dieterich JH, Braun HP, Schmitz UK (2003) Alloplasmic male sterility in Brassica napus (CMS ‘Tournefortii-Stiewe’) is associated with a special gene arrangement around a novel atp9 gene. Mol Genet Genomics 269(6):723–731

    Article  CAS  PubMed  Google Scholar 

  • Ding X, Li J, Zhang H, He T, Han S, Li Y, Yang S, Gai J (2016) Identification of miRNAs and their targets by high-throughput sequencing and degradome analysis in cytoplasmic male-sterile line NJCMS1A and its maintainer NJCMS1B of soybean. BMC Genomics 17:24

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Fan Z, Stefansson BR, Sernyk JL (1986) Maintainers and restorers for three male-sterility-inducing cytoplasms in rape (Brassica napus L.). Can J Plant Sci 66:229–234

    Article  Google Scholar 

  • Feng Y, Zheng Q, Song H, Wang Y, Wang H, Jiang L, Zheng Y, Yue B (2015) Multiple loci not only Rf3 involved in the restoration ability of pollen fertility, anther exsertion and pollen shedding to S type cytoplasmic male sterile in maize. Theor Appl Genet 128:2341–2350

    Article  CAS  PubMed  Google Scholar 

  • Flavell R (1974) A model for the mechanism of cytoplasmic male sterility in plants, with special reference to maize. Plant Sci Lett 3(4):259–263

    Article  Google Scholar 

  • Food and Agriculture Organization (FAO) (2017) FAOSTAT statistical database of the United Nation Food and Agriculture Organization (FAO) Statistical Division, Rome. Available at: http://www.fao.org/faostat/en/#data/QC. Accessed Jan 2019

  • Fu TD (1981) Production and research of rapeseed in the People’s Republic of China. Eucarpia Cruciferae Newslett 6:6–7

    Google Scholar 

  • Fu TD, Yang GS, Yang N (1987) Some investigation on Polima cytoplasmic male sterility in B. napus. Eucarpia Cruciferae Newslett 12:46–47

    Google Scholar 

  • Fujii S, Toriyama K (2008) Genome barriers between nuclei and mitochondria exemplified by cytoplasmic male sterility. Plant Cell Physiol 49:1484–1494

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gaborieau L, Brown GG (2016) Comparative genomic analysis of the compound Brassica napus Rf locus. BMC Genomics 17(1):834

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Grelon M, Bonhomme FBS, Pelletier G (1994) Ogura cytoplasmic male-sterility [CMS]-associated orf138 is translated into a mitochondrial membrane polypeptide in male-sterile Brassica cybrids. Mol Gen Genet 243:540–547

    Article  CAS  PubMed  Google Scholar 

  • Hanson MR, Bentolila S (2004) Interactions of mitochondrial and nuclear genes that affect male gametophyte development. Plant Cell 16:154–169

    Article  Google Scholar 

  • Heng S, Wei C, Jing B, Wan Z, Wen J, Yi B, Ma C, Tu J, Fu T, Shen J (2014) Comparative analysis of mitochondrial genomes between the hau cytoplasmic male sterility (CMS) line and its iso-nuclear maintainer line in Brassica juncea to reveal the origin of the CMS-associated gene orf288. BMC Genomics 15:322

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Heng S, Liu S, Xia C, Tang HY, Xie F, Fu T, Wan Z (2018a) Morphological and genetic characterization of a new cytoplasmic male sterility system (oxa CMS) in stem mustard (Brassica juncea). Theor Appl Genet 131(1):59–66

    Article  PubMed  Google Scholar 

  • Heng S, Liu S, Xia C et al (2018b) Morphological and genetic characterization of a new cytoplasmic male sterility system (oxa CMS) in stem mustard (Brassica juncea). Theor Appl Genet 131(1):59–66

    Article  PubMed  Google Scholar 

  • Heyn FW (1976) Transfer of restorer genes from Raphanus to cytoplasmic male sterile Brassica napus. Cruciferae Newslett 1:15–16

    Google Scholar 

  • Horn R, Gupta KJ, Colombo N (2014) Mitochondrion role in molecular basis of cytoplasmic male sterility. Mitochondrion 19:198–205

    Article  CAS  PubMed  Google Scholar 

  • Hu X, Sullivan-Gilbert M, Kubik T, Danielson J, Hnatiuk N, Marchione W, Greene T, Thompson S (2008) Mapping of the Ogura fertility restorer gene Rfo and development of Rfo allele-specific markers in canola (Brassica napus L.). Mol Breed 22:663–674

    Article  CAS  Google Scholar 

  • Hu J, Huang W, Huang Q, Qin X, Dan Z, Yao G, Zhu R, Zhu Y (2013) The mechanism of ORFH79 suppression with the artificial restorer fertility gene Mt-GRP162. New Phytol 199:52–58

    Article  CAS  PubMed  Google Scholar 

  • Ichii M, Hong DL, Ohara Y, Zhao CM, Taketa S (2003) Characterization of CMS and maintainer lines in indica rice (Oryza sativa L.) based on RAPD marker analysis. Euphytica 129:249–252

    Article  CAS  Google Scholar 

  • Janeja HS, Banga SK, Bhasker PB, Banga SS (2003) Alloplasmic male sterile Brassica napus with Enarthrocarpus lyratus cytoplasm: introgression and molecular mapping of an E. Lyratus chromosome segment carrying a fertility restoring gene. Genome 46:792–797

    Article  CAS  PubMed  Google Scholar 

  • Jarl CI, Bornman CH (1988) Correction of chlorophyll-defective, male sterile winter oilseed rape (Brassica napus) through organelle exchange: phenotypic evaluation of progeny. Hereditas 108:97–102

    Article  Google Scholar 

  • Jiang H, Feng Y, Bao L et al (2012) Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding. Mol Breed 30:1679–1688

    Article  CAS  Google Scholar 

  • Kang L, Li P, Wang A, Ge X, Li Z (2017) A novel cytoplasmic male sterility in Brassica napus (inap CMS) with Carpelloid Stamens via protoplast fusion with Chinese Woad. Front Plant Sci 8:529

    PubMed  PubMed Central  Google Scholar 

  • Kirti PB, Narasimhulu SB, Mohapatra T, Prakash S, Chopra VL (1993) Correction of chlorophyll deficiency in alloplasmic male sterile Brassica juncea through recombination between chloroplast genome. Genet Res 62:11–14

    Article  CAS  Google Scholar 

  • Kirti PB, Banga SS, Prakash S, Chopra VL (1995a) Transfer of Ogu cytoplasmic male sterility to Brassica juncea and improvement of male sterile through somatic cell fusion. Theor Appl Genet 91:517–521

    Article  CAS  PubMed  Google Scholar 

  • Kirti PB, Mohapatra T, Prakash S, Chopra VL (1995b) Development of a stable cytoplasmic male sterile line of Brassica juncea from the somatic hybrid Trachystoma balli + Brassica juncea. Plant Breed 113:21–24

    Google Scholar 

  • Koizuka N, Imai R, Fujimoto H, Hayakawa T, Kimura Y, Kohno-Murase J, Sakai T, Kawasaki S, Imamura J (2003) Genetic characterization of a pentatricopeptide repeat protein gene, orf687, that restores fertility in the cytoplasmic male-sterile Kosena radish. Plant J 34:407–415

    Article  CAS  PubMed  Google Scholar 

  • Kubo T, Kitazaki K, Matsunaga M, Kagami H, Mikami T (2011) Male sterility-inducing mitochondrial genomes: how do they differ? Crit Rev Plant Sci 30:378–400

    Article  CAS  Google Scholar 

  • Kumar P, Vasupalli N, Srinivasan R, Bhat SR (2012) An evolutionarily conserved mitochondrial orf108 is associated with cytoplasmic male sterility in different alloplasmic lines of Brassica juncea and induces male sterility in transgenic Arabidopsis thaliana. J Exp Bot 63:2921–2932

    Article  CAS  PubMed  Google Scholar 

  • L’Homme Y, Stahl RJ, Xiu-Qing Li Hameed A, Brown GG (1997) Brassica napus cytoplasmic male sterility is associated with expression of a mtDNA region containing a chimeric gene similar to the pol CMS-associated orf224 gene. Curr Genet 31:325–335

    Article  PubMed  Google Scholar 

  • Labana KS, Banga SS (1984) Floral biology in Indian mustard (Brassica juncea (L.) Coss). Genet Agrar 38:131–138

    Google Scholar 

  • Landgren M, Zetterstrand M, Sundberg E, Glimelius K (1996) Alloplasmic male-sterile Brassica lines containing B. tournefortii mitochondria express an ORF 3′ of the atp6gene and a 32 kDa protein Plant. Mol Biol 32(5):879–890

    Article  CAS  Google Scholar 

  • Langer S, Longin CFH, Wurschum T (2014) Phenotypic evaluation of floral traits with relevance for hybrid breeding in wheat (Triticum aestivum L.). Plant Breed 133:433–441

    Article  Google Scholar 

  • Liu H, Cui P, Zhan K et al (2011) Comparative analysis of mitochondrial genomes between a wheat K-type cytoplasmic male sterility line and its maintainer line. BMC Genomics 12:163

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu Z, Liu P, Long F, Hong D, He Q, Yang G (2012) Fine mapping and candidate gene analysis of the nuclear restorer gene Rfp for pol CMS in rapeseed (Brassica napus L.). Theor Appl Genet 125:773–779

    Article  CAS  PubMed  Google Scholar 

  • Liu Z, Dong F, Wang X, Wang T, Su R, Hong D, Yang G (2017) A pentatricopeptide repeat protein restores nap cytoplasmic male sterility in Brassica napus. J Exp Bot 68(15):4115–4123

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Longin CF, Reif JC, Wurschum T (2014) Long-term perspective of hybrid versus line breeding in wheat based on quantitative genetic theory. Theor Appl Genet 127:1635–1641

    Article  PubMed  Google Scholar 

  • Luo D, Xu H, Liu Z, Guo J, Li H, Chen L, Fang C, Zhang Q, Bai M, Yao N, Wu H, Wu H, Ji C, Zheng H, Chen Y, Ye S, Li X, Zhao X, Li R, Liu YG (2013) A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice. Nat Genet 45(5):573–577

    Article  CAS  PubMed  Google Scholar 

  • Menczel L, Morgan A, Brown S, Maliga P (1987) Fusion-mediated combination of Ogura-type cytoplasmic male sterility with Brassica napus plastids using X-irradiated CMS protoplasts. Plant Cell Rep 6:98–101

    CAS  PubMed  Google Scholar 

  • Murayama S, Yamagishi H, Terachi T (1999) Identification of RAPD and SCAR markers linked to a restorer gene for Ogura cytoplasmic Male sterility in radish (Raphanus sativus L.) by bulked segregant analysis. Breed Sci 49:115–121

    Article  CAS  Google Scholar 

  • U (1935) Genome analysis in Brassica with special reference to the experimental formation of B. Napus and peculiar mode of fertilization. Jpn J Bot 7:389–452

    Google Scholar 

  • Ngangkham U, Parida SK, De S, Kumar KAR, Singh AK, Singh NK, Mohapatra T (2010) Genic markers for wild abortive (WA) cytoplasm based male sterility and its fertility restoration in rice. Mol Breed 26:275–292

    Article  CAS  Google Scholar 

  • Ogura H (1968) Studies on the new male sterility in Japanese radish, with special references to the utilization of this sterility towards the practical raising of hybrid seeds. Mem Fac Agric Kagoshima Univ 6:39–78

    Google Scholar 

  • Okazaki M, Kazama T, Murata H, Motomura K, Toriyama K (2013) Whole mitochondrial genome sequencing and transcriptional analysis to uncover an RT102-type cytoplasmic male sterility-associated candidate gene derived from Oryza rufipogon. Plant Cell Physiol 54:1560–1568

    Article  CAS  PubMed  Google Scholar 

  • Pathania A, Bhat SR, Dinesh Kumar V et al (2003) Cytoplasmic male sterility in alloplasmic Brassica juncea carrying Diplotaxis catholica cytoplasm: molecular characterization and genetics of fertility restoration. Theor Appl Genet 107(3):455–461

    Article  CAS  PubMed  Google Scholar 

  • Pei X, Jing Z, Tang Z, Zhu Y (2017) Comparative transcriptome analysis provides insight into differentially expressed genes related to cytoplasmic male sterility in broccoli (Brassica oleracea var. italica). Sci Hortic 217:234–242

    Article  CAS  Google Scholar 

  • Pelletier G, Primard C, Vedel F, Chétrit P, Rémy R, Rousselle P, Renard M (1983) Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol Gen Genet 191:244–250

    Article  CAS  Google Scholar 

  • Prakash S, Chopra VL (1988) Synthesis of alloplasmic Brassica campestris as a new source of cytoplasmic male sterility. Plant Breed 101:253–255

    Article  Google Scholar 

  • Prakash S, Kirti PB, Bhat SR, Gaikwad K, Kumar VD, Chopra VL (1998a) A Moricandia arvensis-based cytoplasmic male sterility and fertility restoration system in Brassica juncea. Theor Appl Genet 97:488–492

    Article  CAS  Google Scholar 

  • Prakash S, Kirti P, Bhat S et al (1998b) A Moricandia arvensis–based cytoplasmic male sterility and fertility restoration system in Brassica juncea. Theor Appl Genet 97(3):488–492

    Article  CAS  Google Scholar 

  • Prakash S, Ahuja I, Upreti HC, Dinesh Kumar V, Bhat SR, Kirti PB, Chopra VL (2001) Expression of male sterility in allplasmic Brassica juncea with Erucastrum canariense cytoplasm and the development of a fertility restorer system. Plant Breed 120:479–482

    Article  Google Scholar 

  • Rao GU, Batra VS, Prakash S, Shivanna KR (1994) Development of a new cytoplasmic male sterility system in Brassica juncea through wide hybridization. Plant Breed 112:171–174

    Article  Google Scholar 

  • Rawat DS, Anand IJ (1979) Male sterility in Indian mustard. J Genet Plant Breed 39:412–415

    Google Scholar 

  • Sabar M, Gagliardi D, Balk J, Leaver CJ (2003) ORFB is a subunit of F1F(O)-ATP synthase: insight into the basis of cytoplasmic male sterility in sunflower. EMBO Rep 4:381–386

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sarria R, Lyznik A, Vallejos CE, Mackenzie SA (1998) A cytoplasmic male sterility–associated mitochondrial peptide in common bean is post-translationally regulated. Plant Cell 10:1217–1228

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sheeba NK, Viraktamath BC, Sivaramakrishnan S, Gangashetti MG, Khera P, Sundaram RM (2009) Validation of molecular markers linked to fertility restorer gene(s) for WA-CMS lines of rice. Euphytica 167:217–227

    Article  CAS  Google Scholar 

  • Shi HQ, Gong RF, Zhuan LL, Long XP (1991) Studies on the utilization of heterosis in mustard (Brassica juncea). Acta Agron Sin 17:32–41

    Google Scholar 

  • Shinada T, Kikuchi Y, Fujimoto R, Kishitani S (2006) An alloplasmic male-sterile line of Brassica oleracea harboring the mitochondria from Diplotaxis muralis expresses a novel chimeric open reading frame, orf72. Plant Cell Physiol 47(4):549–553

    Article  CAS  PubMed  Google Scholar 

  • Tan Y, Xu X, Wang C, Cheng G, Li S, Liu X (2015) Molecular characterization and application of a novel cytoplasmic male sterility-associated mitochondrial sequence in rice. BMC Genet 16:45

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tang CY, Yang MK, Wu FY, Zhao H, Pang YJ, Yang RW, Lu GH, Yang YH (2015) Identification of miRNAs and their targets in transgenic Brassica napus and its acceptor (Westar) by high-throughput sequencing and degradome analysis. RSC Adv 5:85383–85394

    Article  CAS  Google Scholar 

  • Tian E, Roslinsky V, Cheng B (2014) Molecular marker-assisted breeding for improved Ogura cms restorer line (RfoRfo) and mapping of the restorer gene (Rfo) in Brassica juncea. Molecular Breed 34(3):1361–1371

    Article  CAS  Google Scholar 

  • Touzet P, Meyer EH (2014) Cytoplasmic male sterility and mitochondrial metabolism in plants. Mitochondrion 19:166–171

    Article  CAS  PubMed  Google Scholar 

  • Walters TW, Mutschler MA, Earle ED (1992) Protoplast fusion-derived Ogura male sterile cauliflower with cold tolerance. Plant Cell Rep 10(12):624–628

    Article  CAS  PubMed  Google Scholar 

  • Warmke HE, Lee SL (1978) Pollen abortion in T cytoplasmic male-sterile corn (Zea mays): a suggested mechanism. Science 200:561–563

    Article  CAS  PubMed  Google Scholar 

  • Xie F, He Z, Esguerra MQ, Qiu F, Ramanathan V (2013) Determination of heterotic groups for tropical Indica hybrid rice germplasm. Theor Appl Genet 127:407–417

    Article  Google Scholar 

  • Yang JH, Zhang MF, Yu JQ, Zhang S, Wang T, Chen ZJ (2005) Identification of alloplasmic cytoplasmic male-sterile line of leaf mustard synthesized by intra-specific hybridization. Plant Sci 168:865–871

    Article  CAS  Google Scholar 

  • Yang J, Liu X, Xu B, Zhao N, Yang X, Zhang M (2013) Identification of miRNAs and their targets using high-throughput sequencing and degradome analysis in cytoplasmic male-sterile and its maintainer fertile lines of Brassica juncea. BMC Genome 14:9

    Article  CAS  Google Scholar 

  • Yuan LP (1985) A concise course in hybrid rice. Hunan Science and Technology Publishing House, Hunan, p 168

    Google Scholar 

  • Zhang H, Wu J, Dai Z, Qin M, Hao L, Ren Y, Li Q, Zhang L (2017) Allelism analysis of BrRfp locus in different restorer lines and map-based cloning of a fertility restorer gene, BrRfp1, for pol CMS in Chinese cabbage (Brassica rapa L.). Theor Appl Genet 130(3):539–547

    Article  CAS  PubMed  Google Scholar 

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Kumar, H., Akhatar, J., Wani, S.H. (2020). Recent Advances in Cytoplasmic Male Sterility (CMS) in Crop Brassicas. In: Wani, S., Thakur, A., Jeshima Khan, Y. (eds) Brassica Improvement. Springer, Cham. https://doi.org/10.1007/978-3-030-34694-2_2

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