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Controlling the Optical Properties of Biological Materials

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The Optical Clearing Method

Part of the book series: SpringerBriefs in Physics ((SpringerBriefs in Physics))

Abstract

To overcome the high light-scattering problem that occurs in biological tissues, we present in this chapter the different clearing methods known today. Most of these methods have benefits and downsides, depending on the application for which they are used. The optical immersion method is introduced as a better, reliable, and reversible way to turn tissues clear. The major benefits and advantages of this method such as its reversibility, the lack of side effects, and application in large wavelength range will be presented. A description of the molecular diffusion of optical clearing agents is given to explain the reduction in the refractive index mismatch that natural tissues have.

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Authors and Affiliations

  1. Physics Department and Center for Innovation in Engineering and Industrial Technology, Polytechnic Institute of Porto – School of Engineering, Porto, Portugal

    Luís Manuel Couto Oliveira

  2. Department of Optics and Biophotonics, Saratov State University, Saratov, Russia

    Valery Victorovich Tuchin

  3. Institute of Precision Mechanics and Control of the RAS, Saratov, Russia

    Valery Victorovich Tuchin

  4. Bach Institute of Biochemistry, Research Center of Biotechnology of the RAS, Moscow, Russia

    Valery Victorovich Tuchin

  5. Tomsk State University, Tomsk & ITMO University, St. Petersburg, Russia

    Valery Victorovich Tuchin

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  1. Luís Manuel Couto Oliveira
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  2. Valery Victorovich Tuchin
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Oliveira, L.M.C., Tuchin, V.V. (2019). Controlling the Optical Properties of Biological Materials. In: The Optical Clearing Method. SpringerBriefs in Physics. Springer, Cham. https://doi.org/10.1007/978-3-030-33055-2_2

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