Abstract
Pathway discovery is a prospective task that is part of the metabolic pathway design workflow. Using the tools that were described in previous chapters to model metabolic networks and chemical diversity, we can now start exploring the metabolic space for routes leading to the production of promising targets. Chemical targets of interest can be identified by performing techno-economic and life cycle analyses. A bioretrosynthesis analysis assesses the existence and feasibility of biosynthetic pathways connecting the target to the chassis. Generalized reaction rules in the bioretrosynthesis analysis can be applied in order to predict de novo or hypothetical routes based on enzyme promiscuity.
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Verified at 7 https://www.aldrichmarketselect.com/
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LCA is one of the requirements in the International standard ISO 14001:2015
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Further Reading
A framework for chemical target selection:
Campodonico, M.A., Sukumara, S., Feist, A.M., Herrgård, M.J.: Computational methods to assess the production potential of bio-based chemicals. In: Methods in molecular biology (Clifton, NJ), vol. 1671, pp. 97–116 (2018)
A more generic discussion about economic considerations for bioprocess commercialization:
Wynn, J.P., Hanchar, R., Kleff, S., Senyk, D., Tiedje, T.: Biobased technology commercialization: the importance of lab to pilot scale-up. In: Metabolic Engineering for Bioprocess Commercialization, pp. 101–119. Springer International Publishing, Cham (2016)
A discussion about biocatalytic retrosynthesis:
Green, A.P., Turner, N.J.: Biocatalytic retrosynthesis: redesigning synthetic routes to high-value chemicals. Perspect. Sci. 9, 42–48 (2016). https://doi.org/10.1016/j.pisc.2016.04.106
A review about pathway design tools:
Wang, L., Dash, S., Ng, C.Y., Maranas, C.D.: A review of computational tools for design and reconstruction of metabolic pathways. Synth. Syst. Biol. 2(4), 243–252 (2017)
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Carbonell, P. (2019). Pathway Discovery. In: Metabolic Pathway Design. Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-030-29865-4_6
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DOI: https://doi.org/10.1007/978-3-030-29865-4_6
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