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Nicotiana benthamiana, A Popular Model for Genome Evolution and Plant–Pathogen Interactions

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The Tobacco Plant Genome

Part of the book series: Compendium of Plant Genomes ((CPG))

Abstract

Nicotiana benthamiana originates from northern Australia and belongs to the Suaveolentes section. It is used extensively as a model organism for many types of research, including plant–pathogen interactions, RNA interference, and functional genomics. Recent publications that used N. benthamiana as a model for plant–pathogen interactions focused mainly on bacteria, viruses, oomycete, and fungi. Two different N. benthamiana whole genome assemblies were published in 2012. These assemblies have been improved and structurally annotated in later versions but are still incomplete. The lineage most widely used in research originates from a population that has retained a loss-of-function mutation in Rdr1 (RNA-dependent RNA polymerase 1) that makes it highly susceptible to viruses. In this chapter, we review some of the techniques used in N. benthamiana to study plant–pathogen interactions, including virus-induced gene silencing, transient protein expression by agroinfiltration, stable genetic manipulation, and transcriptomics analysis, and discuss some of the results. Descriptions and links to some of the most relevant online resources for N. benthamiana are also provided.

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Authors and Affiliations

  1. Instituto de Fisiología Vegetal, INFIVE, Universidad Nacional de La Plata, CONICET, La Plata, Buenos Aires, Argentina

    Marina A. Pombo & Hernan G. Rosli

  2. Faculty of Biology, University of Marburg, Karl-Von-Frisch-Str. 8, 35043, Marburg, Germany

    Noe Fernandez-Pozo

  3. School of Plant and Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA

    Aureliano Bombarely

  4. Dipartimento Di Bioscienze, Universita Degli Studi Di Milano, Milan, Italy

    Aureliano Bombarely

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  1. Marina A. Pombo
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  2. Hernan G. Rosli
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  3. Noe Fernandez-Pozo
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  4. Aureliano Bombarely
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Correspondence to Aureliano Bombarely .

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  1. PMI R&D, Philip Morris Products S.A., Neuchâtel, Switzerland

    Nikolai V. Ivanov

  2. PMI R&D, Philip Morris Products S.A., Neuchâtel, Switzerland

    Nicolas Sierro

  3. PMI R&D, Philip Morris Products S.A., Neuchâtel, Switzerland

    Manuel C. Peitsch

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Pombo, M.A., Rosli, H.G., Fernandez-Pozo, N., Bombarely, A. (2020). Nicotiana benthamiana, A Popular Model for Genome Evolution and Plant–Pathogen Interactions. In: Ivanov, N.V., Sierro, N., Peitsch, M.C. (eds) The Tobacco Plant Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-030-29493-9_14

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